Team:Pretoria UP/Parts

Part Documentation

Part Table

<groupparts>iGEM15 Pretoria_UP</groupparts>

BBa_K1768000

GFP with RBS Reverse Compliment

Description

The reverse compliment of Green fluorescent protein (GFP) Part:BBa_E0040 and and upstream Ribosome Binding Site (RBS) Part:BBa_B0034

BBa_K1768001

Recombinase invertible constitutive promoter 1

Description

A reverse compliment of a constitutive promoter flanked by Lox66 and Lox71 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768003 but Lox66 is upstream of the promoter with a downstream lox71.

BBa_K1768002

Quorum sensing-inducible mxiE transcription factor

Description

The LuxR receptor binds to the lux pR promoter to induce expression of the mxiE transcription factor (BBa_K1325001). mxiE forms a protein-protein interaction with the chaperone protein ipgC (BBa_K1325000) in order to transactivate the pipaH promoter (BBa_K1325004) via an "AND" operation.

BBa_K1768003

Recombinase invertible constitutive promoter 2

Description

A reverse compliment of a constitutive promoter flanked by Lox71 and Lox66 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768001 but Lox71 is upstream of the promoter with a downstream lox66.

BBa_K1768002

Cre with N-terminal His tag

Description

The Cre recombinase protein open reading frame with an N-terminal His protein as an epitope for antibody recognition.