Difference between revisions of "Team:William and Mary"
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| − | <p>As synthetic biology begins to construct increasing complex gene regulatory networks, the need for accurate quantitative characterization of regulatory components becomes more pressing. Despite the extensive characterization of the average strength of the promoters available on the BioBrick registry, very few have information pertaining to the variability in their expression. Our project aims to characterize this variability, commonly referred to stochasticity or noise, in gene expression, for the most commonly used promoters in synthetic biology and provide additional tools for the regulation of these promoters. | + | <p>As synthetic biology begins to construct increasing complex gene regulatory networks, the need for accurate quantitative characterization of regulatory components becomes more pressing. Despite the extensive characterization of the average strength of the promoters available on the BioBrick registry, very few have information pertaining to the variability in their expression. Our project aims to characterize this variability, commonly referred to as stochasticity, or noise, in gene expression, for the most commonly used promoters in synthetic biology and provide additional tools for the regulation of these promoters. |
<p><a href="https://2015.igem.org/Team:William_and_Mary/Description">Read more on our Project Description page.</a> | <p><a href="https://2015.igem.org/Team:William_and_Mary/Description">Read more on our Project Description page.</a> | ||
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Revision as of 18:58, 17 September 2015
NOISE Our Parts In deciding which parts to submit to the iGEM Registry we focused on three main aspects. First: ensuring our project is as reproducible and extensible as possible. To that end we have submitted all of new composite fluorescent protein parts that we constructed during the project. Second: Making genome integration as straightforward as possible for iGEM teams. In order to accomplish this goal we designed, tested, and validated a new integrator cassette that allows for simple genome integration using either 3A or Gibson Assembly. Third: Increasing the number of tools available for promoter-mediated regulation in synthetic biology. We created and validated an E. coli codon optimized dCas9 variant and a suite of gRNAs to target the most commonly used promoters in iGEM. Measurement & Modeling talk a lil about stuff we measured/math model here. 4 components of math model (each will have an icon: Data, analysis, another model, case study) integrated/plasmid parameters and stuff. explain from where applying our model to someone else's stuff Human Practices Our Human Practices effort was a multi-faceted outreach approach to science literacy, focusing specifically on spreading a basic understanding of synthetic biology to the general public. We collaborated with numerous organizations (logos below) to host nine educational Synthetic Biology workshops for the public (from first graders to adults!) and to implement our original, 24-activity Synthetic Biology curriculum into schools worldwide, to further sustain our efforts for years to come. Collaboration W&M iGEM met and exceeded iGEM's collaboration requirements by collaborating with other researchers in four main ways: creating a pen pal program to connect teams with similar projects, participating in the interlab measurement study, interviewing the general public to provide data to future teams about how to communicate synthetic biology, and collaborating on individual research projects with iGEM teams from University of Georgia, University of Maryland, and Cambridge. Team Noise, one promoter at a time.
References Detail1 Detail2 Detail3 Detail4 text text text text etc. text here text hereCharacterization of promoter-derived transcriptional noise in E. coli
Data
analysis
Another Model
Case Study
Project Content.
title
title
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Project Content
title here
