Difference between revisions of "Team:Freiburg/Project/System"

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                                                 <p><strong>Figure 3: Surface protpur.</strong>To prevent unspecific DNA of components of the cell-free expression mix on the glass slide, we established a surface that specifically binds our target proteins, the antigens.</p>
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                                                 <p><strong>Figure 3: Surface protpur.</strong>To prevent unspecific binding of components of the cell-free expression mix on the glass slide, we established a surface that specifically binds our target proteins, the antigens.</p>
  
 
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After cell-free expression not only our desired antigens are present within the chamber, but also all other components of the cell-free mix including ribosomes, polymerases and amino acids (figure 3).  
 
After cell-free expression not only our desired antigens are present within the chamber, but also all other components of the cell-free mix including ribosomes, polymerases and amino acids (figure 3).  
All these components would bind unspecifically to an activated glass slide, thereby obstructing the DNA of the antigens. We designed our DNA constructs in a way that each antigen can easily be fused to specific tags that enable targeted protpur on a specific surface. Testing different tag systems, we found the Ni-NTA-His-tag system to be working best for our purposes. A basic protocol for this <a href="https://2015.igem.org/Team:Freiburg/Results/Surface"target="_blank">specific surface</a> was optimized by ourselves to reduce unspecific DNA.
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All these components would bind unspecifically to an activated glass slide, thereby obstructing the binding of the antigens. We designed our DNA constructs in a way that each antigen can easily be fused to specific tags that enable targeted protpur on a specific surface. Testing different tag systems, we found the Ni-NTA-His-tag system to be working best for our purposes. A basic protocol for this <a href="https://2015.igem.org/Team:Freiburg/Results/Surface"target="_blank">specific surface</a> was optimized by ourselves to reduce unspecific binding.
 
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<h2>Step 4: The Measurement of DNA Events</h2>
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<h2>Step 4: The Measurement of Binding Events</h2>
 
    
 
    
 
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                                                 <p><strong>Figure 4: Optical detection method.</strong> The detection system mainly consists of a camera and an LED and is called <a href="https://2015.igem.org/Team:Freiburg/Project/iRIf"target="_blank">iRIf</a> (imaging Reflectometric Interference). Antigen-Antibody interactions can be detected label-free and in real-time. An optical output of such DNA events is generated by a minimal change in the thickness of the layer on the slide right at the corresponding antigen spot.</p>
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                                                 <p><strong>Figure 4: Optical detection method.</strong> The detection system mainly consists of a camera and an LED and is called <a href="https://2015.igem.org/Team:Freiburg/Project/iRIf"target="_blank">iRIf</a> (imaging Reflectometric Interference). Antigen-Antibody interactions can be detected label-free and in real-time. An optical output of such binding events is generated by a minimal change in the thickness of the layer on the slide right at the corresponding antigen spot.</p>
 
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   After preparation of the DiaCHIP, a patient’s serum sample can be flushed over the protein array using the same microfluidic system. The DNA of antibodies to the corresponding surface causes a minimal change in the thickness of the layer on the slide right at the corresponding antigen spot. This DNA can be detected label-free and in real-time using a novel technique called <a href="https://2015.igem.org/Team:Freiburg/Project/iRIf"target="_blank">iRIf</a> (imaging Reflectometric Interference) without the need for further labeling. Its core components are a camera, an LED and two lenses.  
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   After preparation of the DiaCHIP, a patient’s serum sample can be flushed over the protein array using the same microfluidic system. The binding of antibodies to the corresponding surface causes a minimal change in the thickness of the layer on the slide right at the corresponding antigen spot. This binding can be detected label-free and in real-time using a novel technique called <a href="https://2015.igem.org/Team:Freiburg/Project/iRIf"target="_blank">iRIf</a> (imaging Reflectometric Interference) without the need for further labeling. Its core components are a camera, an LED and two lenses.  
 
See how we reconstructed the system in a <a href="https://2015.igem.org/Team:Freiburg/Results/Own_Device"target="_blank">low-budget device</a>.
 
See how we reconstructed the system in a <a href="https://2015.igem.org/Team:Freiburg/Results/Own_Device"target="_blank">low-budget device</a>.
 
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Revision as of 17:40, 18 September 2015

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in use by DK

The DiaCHIP : Overview

The DiaCHIP is a diagnostic device that offers the possibility for broadband screening for hundreds of diseases simultaneously. It is made up of an antigen array in a microfluidic chamber and can detect diseases via binding of corresponding antibodies. The antigens are fused to a tag and are produced with a cell-free expression mix, using a DNA array as template. The expressed antigens are arranged in an array structure using a specific surface chemistry to stick them to a glass slide. Binding of antibodies can be detected with an optical method, thus providing the possibility of a label-free and real-time analysis.

The Backgrounds of the DiaCHIP

Step 1: Basic Setup of the DiaCHIP

Figure 1: The DiaCHIP is based on antigenic peptides derived from viruses and bacteria.DNA is immobilized on a silicone slide. These sequences are coding for antigens specific for several pathogens. The antigens are expressed by cell-free expression and immobilized on the glass slide.

The aim of our DiaCHIP is to screen simultaneously for hundreds of different infectious diseases. We based our system on the detection of antibodies specifically interacting with antigens derived from viruses and bacteria (figure 1). If you get in contact with one of these pathogens your immune system produces antibodies. These bind specifically to their corresponding antigens. The antigens are produced by using a DNA array as template. The binding of antibody to their corresponding antibodies can be detected with a labelfree detection method. Our approach is based on two components: a silicone slide where DNA coding for distinct antigenic peptides is immobilized and a glass slide with a specific surface to capture the expressed antigens. Both are about the size of a microscopy slide and form a microfluidic chamber. By adding blood of a patient, antibodies that might be present in the sample due to a disease bind to the corresponding antigens.

Step 2: Cell-Free Expressed Proteins

Figure 2: The expression of the antigens is achieved by our cell-free expression mix. This mix is based on a bacterial lysate and contains all components required for transcription and translation of the DNA sequences.

To enable the production of a protein array consisting of multiple antigens on demand, their expression is mediated by cell-free expression from a template DNA array. This expression system based on bacterial lysate makes the need for genetically engineered organisms to produce every single antigen redundant. The protein array is generated by flushing our cell-free expression mix through the microfluidic setup. Expressing the antigens from the DNA template, the protein array is adaptable to individual requirements exhibiting the same pattern for both arrays. Our system is made up of two slides enabling the antigens to be immobilized on the opposite side of the DNA template inside the microfluidic chamber (figure 2).

Step 3: A Specific Surface is Catching the Expressed Protein

Figure 3: Surface protpur.To prevent unspecific binding of components of the cell-free expression mix on the glass slide, we established a surface that specifically binds our target proteins, the antigens.

After cell-free expression not only our desired antigens are present within the chamber, but also all other components of the cell-free mix including ribosomes, polymerases and amino acids (figure 3). All these components would bind unspecifically to an activated glass slide, thereby obstructing the binding of the antigens. We designed our DNA constructs in a way that each antigen can easily be fused to specific tags that enable targeted protpur on a specific surface. Testing different tag systems, we found the Ni-NTA-His-tag system to be working best for our purposes. A basic protocol for this specific surface was optimized by ourselves to reduce unspecific binding.

Step 4: The Measurement of Binding Events

Figure 4: Optical detection method. The detection system mainly consists of a camera and an LED and is called iRIf (imaging Reflectometric Interference). Antigen-Antibody interactions can be detected label-free and in real-time. An optical output of such binding events is generated by a minimal change in the thickness of the layer on the slide right at the corresponding antigen spot.

After preparation of the DiaCHIP, a patient’s serum sample can be flushed over the protein array using the same microfluidic system. The binding of antibodies to the corresponding surface causes a minimal change in the thickness of the layer on the slide right at the corresponding antigen spot. This binding can be detected label-free and in real-time using a novel technique called iRIf (imaging Reflectometric Interference) without the need for further labeling. Its core components are a camera, an LED and two lenses. See how we reconstructed the system in a low-budget device.

Step 5: Changing Perspectives - Off to our Results

Figure 5: Illustration of the perspective during a measurement.

When illustrating the basic principle of the DiaCHIP, we mainly looked at it from the side. Now it is time to explore our results and see what we actually achieved. Therefore, it is important to have in mind that you are observing the chip from the camera's position, so basically from the top (figure 5). This persepective remains the same in all the iRIf measurements we are showing in the results section.

After weeks of optimizing the different components of the DiaCHIP, we are proud to present our results. We reached the highlight of our project with the successful detection of antibodies in our own blood!