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<h5> Recombinase invertible constitutive promoter 2 </5>
 
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A reverse compliment of a constitutive promoter flanked by Lox71 and Lox66 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768001 but Lox71 is upstream of the promoter with a downstream lox66.  
 
A reverse compliment of a constitutive promoter flanked by Lox71 and Lox66 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768001 but Lox71 is upstream of the promoter with a downstream lox66.  
 
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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Revision as of 18:09, 18 September 2015

Part Documentation

Part Table

<groupparts>iGEM15 Pretoria_UP</groupparts>

BBa_K1768000

GFP with RBS Reverse Compliment

Description

The reverse compliment of Green fluorescent protein (GFP) Part:BBa_E0040 and and upstream Ribosome Binding Site (RBS) Part:BBa_B0034

Characterization

Other


BBa_K1768001

Recombinase invertible constitutive promoter 1

Description

A reverse compliment of a constitutive promoter flanked by Lox66 and Lox71 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768003 but Lox66 is upstream of the promoter with a downstream lox71.

Characterization

Other


BBa_K1768002

Quorum sensing-inducible mxiE transcription factor

Description

The LuxR receptor binds to the lux pR promoter to induce expression of the mxiE transcription factor (BBa_K1325001). mxiE forms a protein-protein interaction with the chaperone protein ipgC (BBa_K1325000) in order to transactivate the pipaH promoter (BBa_K1325004) via an "AND" operation.

Characterization

Other


BBa_K1768003

Recombinase invertible constitutive promoter 2

Description

A reverse compliment of a constitutive promoter flanked by Lox71 and Lox66 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768001 but Lox71 is upstream of the promoter with a downstream lox66.


BBa_K1768002

Cre with N-terminal His tag

Description

The Cre recombinase protein open reading frame with an N-terminal His protein as an epitope for antibody recognition.