Difference between revisions of "Team:BABS UNSW Australia/Description"
| Line 5: | Line 5: | ||
<body> | <body> | ||
| − | |||
| − | |||
<br>A LacZ assay was carried out with p170 (<a href="http://parts.igem.org/Part:BBa_K1677300">BBa_K1677300</a>), CP44 (<a href="http://parts.igem.org/Part:BBa_K1677300">BBa_K1677300</a>) and ASR (<a href="http://parts.igem.org/Part:BBa_K1033225">BBa_K1033225</a>) fused to a promoter activity reporter (BBa_I732095) in E. Coli and grown in LB with antibiotics for 8 hours. The data normalized to OD600 of the cultures reveals a decrease in lacZ production correlated with a decrease in pH for the CP44 promoter. A slight increase was observed in both p170 and ASR with the decline in pH, however expression of lacZ is much lower even at neutral pH than with CP44. This characteristic of lowered expression in acidic conditions has previously not been observed with this promoter </br> | <br>A LacZ assay was carried out with p170 (<a href="http://parts.igem.org/Part:BBa_K1677300">BBa_K1677300</a>), CP44 (<a href="http://parts.igem.org/Part:BBa_K1677300">BBa_K1677300</a>) and ASR (<a href="http://parts.igem.org/Part:BBa_K1033225">BBa_K1033225</a>) fused to a promoter activity reporter (BBa_I732095) in E. Coli and grown in LB with antibiotics for 8 hours. The data normalized to OD600 of the cultures reveals a decrease in lacZ production correlated with a decrease in pH for the CP44 promoter. A slight increase was observed in both p170 and ASR with the decline in pH, however expression of lacZ is much lower even at neutral pH than with CP44. This characteristic of lowered expression in acidic conditions has previously not been observed with this promoter </br> | ||
| Line 28: | Line 26: | ||
</body> | </body> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</html> | </html> | ||
{{BABS_UNSW_Australia_Footer}} | {{BABS_UNSW_Australia_Footer}} | ||
Revision as of 18:25, 18 September 2015