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Revision as of 22:46, 18 September 2015

NOISE - W&M iGEM

NOISE

Characterization of promoter-derived transcriptional noise in E. coli

Measurement & Modeling

We measured noise in fluorescence data for dual-integrated sets of CFP and YFP under three promoters: BBa_R0010, BBa_R0011, and BBa_R0051. We also developed an analytic model of the impact of plasmid copy number fluctuations on transcriptional noise, which revealed that intrinsic noise cannot be accurately measured from reporters on the pSB1X3 plasmid series.

Click to go to Measurement

Data & Analysis

integrated/plasmid

Click to go to the Model.

Plasmid Fluctuation Model

parameters and stuff.

Human Practices

Our Human Practices effort was a multi-faceted outreach approach to science literacy, focusing specifically on spreading a basic understanding of synthetic biology to the general public. We collaborated with numerous organizations (logos below) to host nine educational Synthetic Biology workshops for the public (from first graders to adults!) and to implement our original, 24-activity Synthetic Biology curriculum into schools worldwide, to further sustain our efforts for years to come.

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Adult Communication

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Youth Outreach

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Sustainability

Collaboration

W&M iGEM met and exceeded iGEM's collaboration requirements by collaborating with other researchers in four main ways: creating a pen pal program to connect teams with similar projects, participating in the interlab measurement study, interviewing the general public to provide data to future teams about how to communicate synthetic biology, and collaborating on individual research projects with iGEM teams from University of Georgia, University of Maryland, and Cambridge.

Team

Noise, one promoter at a time.

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