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  • Team:Goettingen/Experiments
    ...k() { }); return false;"><h1>PCR product purification using QIAquick&reg; PCR Purification Kit (QIAGEN)</h1></a> ...;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Add 5 volumes Buffer PB to 1 volume of the PCR reaction and mix.</p>
    117 KB (15,375 words) - 00:00, 19 September 2015
  • Template:Team:TU Eindhoven/Protocols HTML
    ...ki/2015/9/96/TU_Eindhoven_Protocols_PCR_Amplification.pdf" target="_blank">PCR Amplification</a> <span class="tekstProtocols">Amplification of DNA can be done using PCR in a very easy way. With the use of a thermal cycler, template DNA and desi
    17 KB (2,479 words) - 21:44, 20 November 2015
  • Template:Heidelberg/notebook/pc/week33
    ...<div class="notebookentry"><h3 class="notebookheading">&#x25BC;2015-08-10 PCR optimized</h3><div class="notebooktext"><table border="1" cellpadding="0" c <p>PCR purification with two of this samples gave to less DNA.</p>
    21 KB (3,282 words) - 09:16, 15 September 2015
  • Team:Brasil-USP/Notebook/protocols
    <li><a href="#directedmutagenesispcr">Directed mutagenesis PCR for restriction site elimination using a plasmid template </a></li> <li><a href="#pcramplification1">PCR amplification (applied to lcp)</a></li>
    24 KB (2,967 words) - 23:52, 20 November 2015
  • Team:Santa Clara/Protocols
    <li>1μl dye to 5μl DNA is sufficient for PCR products and restriction digests.</li> <h4>PCR Components</h4>
    26 KB (2,428 words) - 03:18, 19 September 2015
  • Team:CHINA CD UESTC/Results
    ...e combined <i>mamW+RFP+laccase</i> and <i>RFP</i>+<i>laccase</i> by fusion PCR (Fig. 1B).<br> ...amW</i> and <i>RFP</i> and <i>laccase</i> were amplified by PCR using high fidelity DNA polymerase. <strong>(B)</strong>
    36 KB (3,710 words) - 02:48, 19 September 2015
  • Team:British Columbia/Notebook/Protocols/PhusionPCR
    ...style="width:500px; margin-top:-25px;"><h3>PCR Protocol using Phusion High-Fidelity DNA Polymerase</h3></div> <p><strong>PCR&nbsp;</strong><br />
    8 KB (1,052 words) - 05:45, 22 August 2015
  • Template:Team:Groningen/CONTENT/EXPERIMENTS/Checked Growth
    ...h yuaB forward 2 primer and new suffix primer (figure 1) using NEB Q5 High-Fidelity 2X Master Mix. </div> <div class="caption">Primers for bslA PCR.</div>
    12 KB (1,831 words) - 23:40, 16 September 2015
  • Team:Paris Saclay/Experiments
    <li>Pursue the protocol by the NucleoSpin® Gel and PCR Clean-up protocol</li> <h4>3. PCR amplification</h4>
    17 KB (2,170 words) - 23:29, 18 September 2015
  • Team:NU Kazakhstan/InterLab Study
    ...CGCTACTAGTA) Primers. After that, PCR products were purified with QIAquick PCR Purification Kit. <br> <h3>PCR mixture </h3>
    5 KB (804 words) - 04:39, 16 September 2015
  • Team:Peking/Design/Isothermal
    ...nealing and extension, simulating <i>in vivo</i> DNA replication progress. PCR has radically altered molecular biology; via this technique, a trace amount ...ously. All these methods need a thermal cycler; Nested PCR and Multiplexed PCR need more than two pairs of primers, which may lead to the generation of pr
    39 KB (4,417 words) - 14:54, 17 November 2015
  • Team:Oxford/Notebook/Week1
    <p class="text"><a href="#2206c">PCR Set-up</a></p> ...text"><a href="#2206d">Setting up Agarose Gel for Electrophoresis of 22/06 PCR Products</a></p>
    14 KB (1,920 words) - 15:44, 6 July 2015
  • Team:BIT-China/project Fine-regulation circuits.html
    ...of PoxB, Pta, sdhA and some other enzymes could be knocked out to ensure a high succinic acid yield <sup>[15]</sup>. <!-- Error-Prone PCR -->
    43 KB (6,183 words) - 20:20, 18 September 2015
  • Template:Heidelberg/notebook/pc/week28
    ...eaction with Thermo 2x Phusion Flash PCR Mastermix and with NEB Q5 2x High-Fidelity Mastermix.</p> <p>The insert fragments were amplified using the Thermo 2x Phusion Flash PCR Mastermix.</p>
    7 KB (1,199 words) - 09:15, 15 September 2015
  • Team:UCLA/Notebook
    <li>PCR'd off template</li> <p>PCR Reaction:</p>
    12 KB (1,619 words) - 23:38, 20 May 2015
  • Team:KU Leuven/Notebook/Newsfeed
    ...ks LuxI-His, RBS-LuxI-His and CheZ-GFP, RBS-CheZ-GFP by high fidelity tail-PCR, digestion and ligation in pSB1C3<br/> ...>, testing colonies by PCR and preparing for sequencing. The high fidelity PCR and cloning of other potential BioBricks was repeated. <br/>
    58 KB (8,236 words) - 10:16, 20 October 2015
  • Team:Freiburg/Protocols/PCR
    <h1 class="sectionedit1"><a name="pcr" id="pcr">PCR</a></h1> <!-- EDIT1 SECTION "PCR" [1-19] -->
    5 KB (690 words) - 07:05, 20 November 2015
  • Team:Hong Kong HKU/Experiments
    <h6> 12. Add 50 uL PCR water to the center of the QIAprep spin column to elute DNA, let stand for <h2>PCR using Q5 High-Fidelity DNA Polymerase</h2>
    25 KB (3,384 words) - 14:18, 17 September 2015
  • Team:Example/Experiments/ex
    <h6> 12. Add 50 uL PCR water to the center of the QIAprep spin column to elute DNA, let stand for <h2>PCR using Q5 High-Fidelity DNA Polymerase</h2>
    31 KB (4,008 words) - 12:01, 14 September 2015
  • Team:UCLA/Notebook/Interlab Study/14 August 2015
    = PCR Amplification and Gel Extraction of InterLab Devices = == PCR Reaction & Thermocycler Setup ==
    14 KB (1,900 words) - 01:48, 16 August 2015

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