Team:UiOslo Norway/Experiments

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Protocols

Molecular biology

• PCR with Phusion-HF DNA Polymerase (NEB)

• PCR with Taq DNA Polymerase (NEB)

• Restriction Digest

• Shrimp Alkaline Phosphatase Treatment (Fermentas)

• Ligation with T4 DNA Polymerase (NEB)

• TOPO Zero blunt cloning (Invitrogen)

• Wizard® SV Gel and PCR Clean-Up System (Promega)

• QIAprep Spin Miniprep Kit (Qiagen)

• NucleoSpin® Tissue (Machery-Nagle)

• In vitro Mutagenesis

• Agarose Gel

• List of primers

Protein expression

• Protein Expression

• Solubility Assay

• SDS-Page

Handling bacteria

• E. coli strains

• Transformation of chemically competent E. coli cells

• Antibiotic Concentrations

• LB media

• NMS medium

• NMS-medium for growth of Methylococcus capsulatus (Bath)