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Labjournal Cellfree September

Fri, 04.09.

Cell-free Expression of HA-GFP-His6-His6, His10-GFP-Spy and GFP Bielefeld with KK and Promega

(Luisa)

• 50 µl reactions were incubated at 37°C in the platereader (480/520 nm) for 4 hours in a 384 well plate. afterwards the foil covering the plate was removed a spectrum was obtained
• comparison to GFP 1mg/ml dilutions
• plate layout:

	1	2	3	4	5
A	HA-GFP-His6-His6 (KK)	His10-GFP-Spy (KK)	GFP Bielefeld (KK)	no DNA (KK)	air
B	HA-GFP-His6-His6 (KK)	His10-GFP-Spy (KK)	GFP Bielefeld (KK)	no DNA (KK)	air
C	HA-GFP-His6-His6 (KK)	His10-GFP-Spy (KK)	GFP Bielefeld (KK)	no DNA (KK)	air
D	HA-GFP-His6-His6 (PP)	His10-GFP-Spy (PP)	GFP Bielefeld (PP)	no DNA (PP)	air
E	HA-GFP-His6-His6 (PP)	His10-GFP-Spy (PP)	GFP Bielefeld (PP)	no DNA (PP)	air
F	HA-GFP-His6-His6 (PP)	His10-GFP-Spy (PP)	GFP Bielefeld (PP)	no DNA (PP)	air
G	GFP 1:10	GFP 1:20	GFP 1:40	GFP 1:80	air