Team:Freiburg/Project/pRIG15 7

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pRIG15_7

The Varicella Zoster Virus also called Human Herpes Virus Type 3 causes chickenpox after first infection and remains as a latent infection. The sequence we used during our project codes for the extracellular part of the glycoprotein E ¹⁾, which is the most abundant glycoprotein on the surface of infected cells. ²⁾

To insert the sequence for Varicella Zoster glycoprotein E into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. This fragment was amplified via PCR (Link zum Labjournal-Eintrag) and then assembled with the digested pSB1C3 backbone using Gibson assembly. To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing.

¹⁾ Thomsson et al. 2011. Recombinant glycoprotein E produced in mammalian cells in large-scale as an antigen for varicella-zoster-virus serology. Journal of virological methods

²⁾ Mo et al. 2003. Varicella-zoster virus infection facilitates VZV glycoprotein E trafficking to the membrane surface of melanoma cells. Journal of medical virology