Team:BABS UNSW Australia/project/phlow
Overview
The two proteins essential for bacterial invasion of mammalian cytoplasm, invasin and listeriolysin, were already in the registry, and were previously shown to work in a very simple genetic circuit - promoter-rbs-protein-rbs-protein. Our aim with the ‘pHlow’ system was to more finely tune the expression of these proteins, as well as improve the biosafety of modified organisms.
Acid shock response promoter
An essential component of the pHlow plasmid is listeriolysin only being expressed once the cell in within the lysosome. Bacteria often are subjected to environmental stressors in the form of pH change, requiring differential gene expression, and hence have pH specific promoters. We have had these endogenous promoters synthesized and have characterised them for use in the pHlow plasmid.
Pseudoknots
wait are pseudoknots even going here or in a section of their own or both???