Team:ITB INDONESIA/idea/production-module
RhamCOLIpid
Engineered E. coli producing rhamnolipid for green enhanced oil recovery
Production Module
Part submission tim ITB_Indonesia for production module, Rhamnolipid Source: http://parts.igem.org/Part:BBa_K1685011
There are two enzymes responsible for rhamnolipid production in Pseudomonas aeruginosa. The first enzyme, RhlA, catalyses dimerization of two hydroxyacyl (usually hydroxydecanoyl) chains that will constitute rhamnolipid’s lipid moiety, HAA. RhlB will catalyse the transfer of rhamnose and HAA forming mono-rhamnolipid.
We utilise The Biosurfactor biobrick (BBa_K653000) designed by Panama 2011 that encodes rhlA and rhlB. We put the biobrick under T7lac promoter with strong RBS, and double terminator.