Team:ITB INDONESIA/Attributions
RhamCOLIpid
Engineered E. coli producing rhamnolipid for green enhanced oil recoveryAttributions
We would like to thank all those who have contributed to the making of this project.
Attributions
All the wet lab work, human practice, modelling, logo and wiki were done by the students in the team, with guidance and support from people mentioned below.
Project References
- Rhamnolipid biosynthesis, analysis, etc: Abdel-Mawgoud et al. 2011. Rhamnolipids: Detection, Analysis, Biosynthesis, Genetic Regulation, and Bioengineering of Production. in G. Soberon‐Chavez (ed.), Biosurfactants, Microbiology Monographs 20. Springer-Verlag Berlin, Heidelberg.
- Initial rhamnolipid production's modeling: Henkel et al. 2014. Kinetic modeling of rhamnolipid production by Pseudomonas aeruginosa PAO1 including cell density-dependent regulation. Appl Microbiol Biotechnol. DOI 10.1007/s00253-014-5750-3.
- Rhamnolipid production in E. coli: Cabrera-Valladares et al. 2006. Monorhamnolipids and 3-(3-hydroxyalkanoyloxy) alkanoic acids (HAAs) production using Escherichia coli as a heterologous host. Appl Microbiol Biotechnol 73:187–194.
- Rhamnolipid activity test: Peter & Singh. 2014. Characterization of emulsification activity of partially purified Rhamnolipids from Pseudomonas fluorescens. IJISR 3(1):88-100
Lab support
- Head of Genetics and Molecular Biotechnology Laboratory from School of Life Sciences and Technology, head of Biochemistry Laboratory from Chemistry Department, head of Biotech Pharmacy Laboratory from School of Pharmacy, Institut Teknologi Bandung, provided us with equipment and lab spaces for wet lab work.
- Cut Nanda Sari, M.Sc from Chemical Flooding Laboratory, Research and Development Centre for Oil and Gas Technology, Ir. Letty Brioletty, M.Sc., Head of EOR Research Division, and also Putri, S.T., researcher, helped us to measure the interfacial surface tension and the density of our RhamColipid.
- Tati Kristianti, M.Sc from Genetics and Molecular Biotechnology Laboratory, Institut Teknologi Bandung, supported our wet lab work.
- Yessi Permana, Dr. Eng. from Chemistry Department, Inorganic Chemistry Research Division, Institut Teknologi Bandung, gave us the permission to measure FT-IR at the lab and also Elevia Febriani, S.Si., helped us in the FT-IR measurement of our samples.
- Dr. Made Puspasari Widhiastuty, from Chemistry Department, Biochemistry Research Division as the Head of Biochemistry Lab, gave us the permission to measure the fluorescence activity of our samples, and also Bu Diah, the analyst from the Biochemistry Lab, for the advices.
- Ari Dwijayanti, M.Sc helped us to remind the important dates.
Human practices support
- Ana Sifuentes, as the Representative of iGEM Headquarter, shared and gave the audiences of our synhetic biology seminar and meetup about the iGEM.
- Indra Wibowo, Ph.D from School of Life Sciences and Technology, Institut Teknologi Bandung, helped us to develop our human practices project from biosafety view.
- Muhamad Sahlan, Ph.D from Chemical Engineering Department, Universitas Indonesia, joined our synthetic biology seminar and meet up as a speaker.
- Dr. Tri Cahyanto, S.Pd., M.Si., Ana Widiana, M.Si., and Anggita, M.Si from Biology Department, Universitas Islam Negeri Sunan Gunung Djati Bandung, supported us to hold a seminar and workshop entitled “Biodiversity as a Basic Knowledge for Synthetic Biology”.
- Topik Hidayat, Ph.D and his student, Hilal, from Biology Department of Universitas Pendidikan Indonesia, helped us to introduce synthetic biology in their department.
- Ir. Leksono Mucharam, Ph.D from Petroleum Engineering, Institut Teknologi Bandung, as an expert in EOR, informed us the works of surfactant and its prerequisite as a good biosurfactant for Enhance Oil Recovery (EOR).
- Ir. Letty Briolettey, M.Sc., Head of EOR Research Division, R & D Center for Oil and Gas Technology (LEMIGAS), gave us insights about EOR, surfactant and its application in the industry, gave us many insights about the EOR application.
- Ir. Novyal Erwin, Ex Site Manager from Total E & P Indonesie, overview the utilization of biosurfactant in oil and gas industry.
- Tirta Widi Gilang from SynBio Club, helped us to share our project to the members of SynBio Club Institut Teknologi Bandung.
- Muhammad Ridha Ridwan from Interior Design, Institut Teknologi Bandung, helped us to visualize our project in form of video.
- UB_Indonesia iGEM 2015 team, joined our meet up collaboration entitled “Seminar and Meet Up iGEM 2015 Indonesia Team”. Lasts, trusted our team to help their wet lab work.
- UI_Indonesia iGEM 2015 team, joined our meet up collaboration entitled “Seminar and Meet Up iGEM 2015 Indonesia Team”.
- Genecraft, supported our seminar entitled “Seminar and Meet Up iGEM 2015 Indonesia Team”.
- Riandy Rahman Nugraha and Andre Susanto, as the contibutors, helped us to overcome the problem faced by the Human Practice blog: http://synbio.itb.ac.id.
- All of the respondents who are participating in our study and survey.
- The Oxford iGEM team, helped us for the distribution of our questionnaires.
- BioBrick Seeker tool developed by the 2014 Aalto-Helsinki team and updated by the 2015 team
Fundraising Help
- Dr. Adi Pancoro from School of Life Sciences and Technology, Institut Teknologi Bandung, helped us with fundraising.
- Dr. Agus Dana Permana from School of Life Sciences and Technology, Institut Teknologi Bandung, helped us with finding contacts in oil and gas companies.
Our sponsors
- The Office of Student Affairs Institut Teknologi Bandung. (http://kemahasiswaan.itb.ac.id/web/)
- The Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung. (http://www.fmipa.itb.ac.id/en/)
- School of Life Sciences and Technology, Institut Teknologi Bandung. (http://www.sith.itb.ac.id/en/)
- PT Pupuk Indonesia (Persero), formerly known as PT Pusri (Persero), is the first fertilizer manufacturer state-owned enterprise (BUMN) in Indonesia. Pupuk Indonesia Holding Company vision is committed to become an integrated , sustainable, and world-class agrochemical company. Pupuk Indonesia supported us financially. (http://pupuk-indonesia.com/en/)
- Integrated DNA Technologies (IDT) supported us for 20kb fragment DNA (http://idtdna.com)