Team:ITB INDONESIA/protocol

RhamCOLIpid

Engineered E. coli producing rhamnolipid for green enhanced oil recovery

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Protocols

Here are some of the protocols that we used during our lab sessions :

• Antibiotic for plasmid selection
• DNA plasmid Isolation using alkaline lysis
• CCMB80 buffer solution
• Competent cells
• Colony PCR
• Transformation
• 3A assembly
• Autoinduction medium preparation
• Rhamnolipid emulsification activity assay

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