Team:Dundee/Safety
Abstract
Lanosterol synthase (LSS) an oxidosqualene cyclase (OSC) enzyme that specifically binds to squalene epoxide (2,3- oxidosqualene), which is present in fingerprints. We successfully managed to clone lanosterol synthase into pSB1C3 and the over expression vector pQE80-L and successfully characterised using an anti-His antibody. We then continued on in the hope to purify this enzyme from E. coli using Nickel affinity chromatography.
Introduction
Future Work
Figure 7 -Crystal structure of lanosterol synthase with the active sites at positions 232 and 455 (red). The yellow arrows indicate the active sites in laonsterol synthase that will have to be mutated to inactivate the enzyme to avoid unwanted activity.
