User contributions
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- 21:23, 6 September 2015 (diff | hist) . . (+873) . . Team:UCLA/Notebook/Spider Silk Genetics/2 September 2015
- 19:47, 6 September 2015 (diff | hist) . . (+212) . . N Team:UCLA/Notebook/Spider Silk Genetics/2 September 2015 (Created page with "{{Template:UCLA}} =9/2/2015= ==Sequencing Results== *M1-12(1C3) **1: GOOD **2: Bad, deletion in suffix **3: GOOD *M1-12(T7) **1: incorrect sequence **2: GOOD **3: Bad, no rever...")
- 19:40, 6 September 2015 (diff | hist) . . (+185) . . Team:UCLA/Notebook/Spider Silk Genetics
- 19:38, 6 September 2015 (diff | hist) . . (+1,252) . . N Team:UCLA/Notebook/Spider Silk Genetics/1 September 2015 (Created page with "{{Template:UCLA}} =9/1/2015= ==Miniprep== *Used qiagen kit to prep M1-12(1C3)/(T7) and M1/2[1:2]-12(1C3)/(T7) *Sent 3 samples of each for sequencing (Fasih prepped) ==ICA for ...") (current)
- 19:35, 6 September 2015 (diff | hist) . . (0) . . N File:9 1 2015 UCLA ICA.jpg (current)
- 06:50, 6 September 2015 (diff | hist) . . (+156) . . Team:UCLA/Notebook/Spider Silk Genetics
- 06:11, 6 September 2015 (diff | hist) . . (+844) . . N Team:UCLA/Notebook/Spider Silk Genetics/31 August 2015 (Created page with "{{Template:UCLA}} =8/31/2015= ==Transformation Results== *All plates have colonies! ==Starter Cultures Analysis== *Starter cultures grown at 30 C are overgrown. *Cultures at 2...") (current)
- 06:09, 6 September 2015 (diff | hist) . . (0) . . N File:8 31 2015 UCLA ICA.jpg (current)
- 05:49, 6 September 2015 (diff | hist) . . (+230) . . Team:UCLA/Notebook/Spider Silk Genetics
- 05:37, 6 September 2015 (diff | hist) . . (+960) . . Team:UCLA/Notebook/Spider Silk Genetics/30 August 2015 (current)
- 04:44, 6 September 2015 (diff | hist) . . (+188) . . Team:UCLA/Notebook/Spider Silk Genetics
- 04:43, 6 September 2015 (diff | hist) . . (+1,335) . . N Team:UCLA/Notebook/Spider Silk Genetics/28 August 2015 (Created page with "{{Template:UCLA}} =8/28/2015= ==Gel Purification for M1 monomers== *Used zymo kit, eluted in 12 uL of solution. *Yields were typical, around 25 ng/uL ==Sequencing for M1, M2 m...") (current)
- 04:39, 6 September 2015 (diff | hist) . . (0) . . N File:8 28 2015 UCLA ICA.jpg (current)
- 04:33, 6 September 2015 (diff | hist) . . (0) . . Team:UCLA/Notebook/Spider Silk Genetics/25 August 2015 (current)
- 04:20, 6 September 2015 (diff | hist) . . (+183) . . Team:UCLA/Notebook/Spider Silk Genetics
- 04:19, 6 September 2015 (diff | hist) . . (+889) . . Team:UCLA/Notebook/Spider Silk Genetics/27 August 2015 (current)
- 04:18, 6 September 2015 (diff | hist) . . (0) . . N File:8 27 2015 UCLA ICA BsaIdigest.jpg (current)
- 04:07, 6 September 2015 (diff | hist) . . (0) . . N File:8 27 2015 UCLA ICA XPdigest.jpg (current)
- 04:02, 6 September 2015 (diff | hist) . . (+984) . . N Team:UCLA/Notebook/Spider Silk Genetics/27 August 2015 (Created page with "{{Template:UCLA}} =8/27/2015= ==Sequencing Results== *M1-12(1C3) **1: wrong sequence entirely **2: wrong sequence entirely **3: wrong sequence entirely **Interestingly, all thr...")
- 04:02, 6 September 2015 (diff | hist) . . (0) . . Team:UCLA/Notebook/Spider Silk Genetics
- 02:37, 6 September 2015 (diff | hist) . . (+668) . . N Team:UCLA/Notebook/Spider Silk Genetics/30 August 2015 (Created page with "{{Template:UCLA}} =8/27/2015= ==Sequencing Results== *M1-12(1C3) **1: wrong sequence entirely **2: wrong sequence entirely **3: wrong sequence entirely **Interestingly, all thr...")
- 02:20, 6 September 2015 (diff | hist) . . (+188) . . Team:UCLA/Notebook/Spider Silk Genetics
- 18:50, 31 August 2015 (diff | hist) . . (-1) . . Team:UCLA/Notebook/Spider Silk Genetics/26 August 2015 (current)
- 16:21, 31 August 2015 (diff | hist) . . (+437) . . N Team:UCLA/Notebook/Spider Silk Genetics/26 August 2015 (Created page with "{{Template:UCLA}}} =8/26/2015= ==Transformation Results== *Only 2 colonies present on the M1/2[1:2]-12(1C3) plate. *Possibly due to DNA loss during PCR purification. ==Minipre...")
- 22:15, 30 August 2015 (diff | hist) . . (+1) . . Team:UCLA/Notebook/Spider Silk Genetics
- 22:14, 30 August 2015 (diff | hist) . . (-1) . . Team:UCLA/Notebook/Spider Silk Genetics
- 22:14, 30 August 2015 (diff | hist) . . (+161) . . Team:UCLA/Notebook/Spider Silk Genetics
- 22:01, 30 August 2015 (diff | hist) . . (+1,610) . . N Team:UCLA/Notebook/Spider Silk Genetics/25 August 2015 (Created page with "{{Template:UCLA}} =8/25/2015= ==Transformation Results== *M1-12, M1/2[1:1]-12(1C3)/(T7), M1/2[2:1]-12(1C3)/(T7) all have several dozens of colonies. ==PCR amplification for M1...")
- 18:12, 30 August 2015 (diff | hist) . . (+268) . . Team:UCLA/Notebook/Spider Silk Genetics
- 18:10, 30 August 2015 (diff | hist) . . (+57) . . Team:UCLA/Notebook/Spider Silk Genetics/24 August 2015 (→Transformation) (current)
- 18:09, 30 August 2015 (diff | hist) . . (+965) . . N Team:UCLA/Notebook/Spider Silk Genetics/24 August 2015 (Created page with "{{Template:UCLA}} =8/24/2015= *Gel extraction for M1/2[1:1]-12, M1/2[2:1]-12 ==Digestion for M1/2[1:1]-12, M1/2[2:1]-12== *2 reactions for each. *Digest one reaction with E, P...")
- 23:17, 29 August 2015 (diff | hist) . . (+89) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols/Iterative Capped Assembly (→ICA Product Amplification)
- 23:17, 29 August 2015 (diff | hist) . . (+212) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols/Iterative Capped Assembly
- 23:13, 29 August 2015 (diff | hist) . . (+295) . . Team:UCLA/Notebook/Spider Silk Genetics/ICA Oligo Sequences
- 22:25, 29 August 2015 (diff | hist) . . (+129) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols/Iterative Capped Assembly (→Iterative Capped Assembly)
- 22:22, 29 August 2015 (diff | hist) . . (+806) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols/Iterative Capped Assembly (→Iterative Capped Assembly)
- 22:05, 29 August 2015 (diff | hist) . . (+104) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols (→Spider Silk Genetics Protocols)
- 22:05, 29 August 2015 (diff | hist) . . (+137) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols (→Spider Silk Genetics Protocols)
- 16:38, 26 August 2015 (diff | hist) . . (+1,217) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols/Iterative Capped Assembly
- 16:25, 25 August 2015 (diff | hist) . . (+694) . . N Team:UCLA/Notebook/Spider Silk Genetics/Protocols/Iterative Capped Assembly (Created page with "{{Template:UCLA}} This document details steps to perform ICA for Spider Silk Gene Assembly. =ICA Preparation= *[https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/Pro...")
- 16:23, 25 August 2015 (diff | hist) . . (-1) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols
- 16:22, 25 August 2015 (diff | hist) . . (+105) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols/ICA Preparation (→Initiator, Terminator, and Capping Oligo Preparation) (current)
- 16:18, 25 August 2015 (diff | hist) . . (+65) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols/ICA Preparation (→ICA Elution Buffer)
- 16:17, 25 August 2015 (diff | hist) . . (+106) . . Team:UCLA/Notebook/Spider Silk Genetics/Protocols (→Spider Silk Genetics Protocols)
- 16:16, 25 August 2015 (diff | hist) . . (+891) . . N Team:UCLA/Notebook/Spider Silk Genetics/Protocols/ICA Preparation (Created page with "{{Template:UCLA}} This page details solutions and reagents that should be prepared prior to beginning Iterative Capped Assembly. =Solutions= ==2x Binding and Wash (BW) Buffer==...")
- 07:13, 24 August 2015 (diff | hist) . . (+1,842) . . Team:UCLA/Notebook/Spider Silk Genetics
- 06:40, 24 August 2015 (diff | hist) . . (+2,140) . . N Team:UCLA/Notebook/Spider Silk Genetics/21 August 2015 (Created page with "{{Template:UCLA}} =8/21/2015= ==Gel Purification for M2 monomers== *Used Zymo Kit. Concentrations around 25 ng/uL. ==ICA for M1/2[2:1]-12== *Used same ICA protocol as previous...") (current)
- 06:34, 24 August 2015 (diff | hist) . . (0) . . N File:8 21 2015 UCLA ICA.jpg (current)
- 05:55, 24 August 2015 (diff | hist) . . (+1,792) . . N Team:UCLA/Notebook/Spider Silk Genetics/20 August 2015 (Created page with "{{Template:UCLA}} =8/20/2015= ==Size Verification for M1-12(1C3)== *Digested 1 ug of each of the sequence verified plasmid from [https://2015.igem.org/Team:UCLA/Notebook/Spider...") (current)
- 05:54, 24 August 2015 (diff | hist) . . (0) . . N File:8 20 2015 UCLA BsaI.jpg (current)
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