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• Team:Paris Bettencourt/jb
  <span class="c2">HO-Poly-KanMX4-HO amplification (reverse) + tail</span> <span class="c2">gBlock2 amplification (forward) + tail</span>
  15 KB (1,977 words) - 15:31, 14 August 2015
• Team:Birkbeck/Basic Parts
  <p>This is host-specific subunit of the short tail fibre protein of bacteriophage lambda. The C-terminus of the protein (ORF-3 <h3><b>stf (short tail fibre) gene (BBa_K1846004)</b></h3>
  8 KB (1,199 words) - 21:29, 16 November 2015
• Team:XJTLU-CHINA/Description
  ...en color of inland area on the earth. Both chromoprotein followed with aav tail to obtain the color change distinctly from green to yellow. We introduced a ...the chromoprotein to make it more vulnerable to the attack from endogenous tail-specific proteases. Two tails were chosen for our testing, LVA tag and AAV
  72 KB (7,426 words) - 16:17, 4 October 2015
• Team:Birkbeck/Composite Part
  <h3><b>tfa (tail fibre assembly) gene circuit (BBa_K1846001)</b></h3> <p>This part provides the gene sequence for tfa (tail fibre assembly) protein, together with a TetR repressible promoter (<a href
  11 KB (1,716 words) - 21:16, 16 November 2015
• Team:Chalmers-Gothenburg/OtherApplications
  ...d be translated into the protein. Second, the gRNA must be free from polyA tail and 5’ cap to avoid relocation into the cytoplasm and degradation</p> ...The other CGC1 part, the synthetic poly A tail, is used as the new poly-A tail after ribozyme cleavage ensuring the cas9 mRNA translocation and translatio
  8 KB (1,343 words) - 03:31, 19 September 2015
• Team:Exeter/Practices
  ...w cows are much more accepting of a vet approaching and working with their tail as oppose to their necks.</p> ...tically Phil said this would not be possible. Collection of blood from the tail into a tube the size of an Eppendorf would not only be extremely difficult
  46 KB (7,231 words) - 03:08, 19 September 2015
• Team:Technion HS Israel/Constants Database
  <tr><td>398</td><td>L</td><td>tail length</td><td>0.8</td><td>cm</td><td><a href="#atlas">[1]</a></td><td><a h <tr><td>399</td><td>l</td><td>max tail width</td><td>0.07</td><td>cm</td><td><a href="#atlas">[1]</a></td><td><a h
  265 KB (43,510 words) - 20:28, 18 September 2015
• Team:Dundee/Forensic Toolkit/FluID
  ...eraction, the larger beta barrel is shown in blue and the small c-terminal tail show in red.</p> ...f OBP2A in exchange for our tighter binding synthetic tail. This synthetic tail could then be attached to a florescent nanoparticle and used in the detecti
  41 KB (6,123 words) - 12:47, 4 October 2015
• Team:Paris Bettencourt/Notebook/Phytase
  <br><span style="color:#9A1717"> -&nbsp;Homology tail on gene PHO85</span> <br><span style="color:#9A1717"> -&nbsp;Homology tail on gene PHO80</span>
  26 KB (4,138 words) - 14:04, 30 October 2015
• Team:Birkbeck/Composite Parts
  <h3>TFA (tail fibre assembly) gene circuit (BBa_K1846001)</h3> ...ts in the assembly of the stf (short tail fibre) protein into a functional tail fibre. This part provides the gene sequence for tfa, together with a TetR r
  2 KB (308 words) - 17:50, 28 August 2015
• Team:NAIT Edmonton/Basic Part
  ...le pork belly ball tip short ribs biltong meatball chuck. Pork chop ribeye tail short ribs, beef hamburger meatball kielbasa rump corned beef porchetta lan ...k ribeye landjaeger beef kevin tail meatball pastrami prosciutto pancetta. Tail kevin spare ribs ground round ham ham hock brisket shoulder. Corned beef tr
  9 KB (1,220 words) - 21:33, 1 September 2015
• Team:Exeter/PhilL
  ...w cows are much more accepting of a vet approaching and working with their tail as oppose to their necks. </p> ...tically Phil said this would not be possible. Collection of blood from the tail into a tube the size of an Eppendorf would not only be extremely difficult
  2 KB (404 words) - 13:16, 9 September 2015
• Team:Birkbeck/Notebook
  ...reating some BioBricks. First up, the most basic one: ORF314, (a piece of) tail fibre protein of lambda phage. After cloning the gene into iGEM standard ba ...by the end of the week we would have a complete bacteriophage lambda stf (tail fibre protein) gene with a His-tag for easy purification. But alas! In typi
  33 KB (4,872 words) - 21:27, 16 November 2015
• Team:KU Leuven/Notebook/Newsfeed
  ...oBricks LuxI-His, RBS-LuxI-His and CheZ-GFP, RBS-CheZ-GFP by high fidelity tail-PCR, digestion and ligation in pSB1C3<br/> - Optimizing the tail PCR to make Biobricks <br/>
  58 KB (8,236 words) - 10:16, 20 October 2015
• Team:EPF Lausanne/Project/Description
  <li> The polyA tail is a sequence of 50 adenines. This particular sequence aims to replace the <br>[4] [4] Preiss T, Muckenthaler M, Hentze MW. Poly(A)-tail-promoted translation in yeast: Implications for translational control. 1998
  55 KB (8,059 words) - 23:46, 7 October 2015
• Team:Uppsala/Biosurfactants
  ...rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail which can vary in length (Han <i>et al</i>. 2014). The mono-rhamnolipid wil ...efficiency. The RhlA creates the fatty acid tail and the RhlB connects the tail to a rhamnose molecule. (Han <i>et al</i>. 2014) The genes that express the
  12 KB (1,820 words) - 21:58, 19 November 2015
• Team:UCLA/Project/Programming Spider Silk
  ...non-specificity in primer binding. While other techniques such as head-to-tail assembly or concatemerization have been developed to facilitate spider silk ...ic sequences, then assemble the monomers into the final construct. Head-to-tail cloning assembles gene constructs by ligating two plasmid halves together.
  20 KB (3,083 words) - 21:45, 18 September 2015
• Team:Chalmers-Gothenburg/Theory
  ...wer, K., Kuperwasser, N., Merrikh, H., & Rosbash, M. (2004). A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript. <I> ...short DNA duplexes with at least a 10 nucleotide long 5’single-stranded tail for tight binding and efficient unwinding. [1]</p>
  54 KB (8,522 words) - 02:18, 19 September 2015
• Team:Birkbeck/Description
  ...pecificity is <a href="https://2015.igem.org/Team:Birkbeck/Composite_Part">tail fibre assembly (TFA) protein</a>.</p>
  4 KB (630 words) - 21:56, 18 September 2015
• Team:Birkbeck/Basic Part
  ...li. Furthermore, the sequence shows great (>50%) homology with the gene 37 tail fibre protein of bacteriophage T4.</p>
  2 KB (295 words) - 12:04, 11 August 2015
• Team:Birkbeck/Modeling
  <td>Short tail fibre (stf) and reporter genes are cloned into a gt11 bacteriophage lambda <td>Regulatory circuits are created to control the production of the tail fibre assembly (tfa) protein and the switch between lytic/lysogenic cycles
  5 KB (763 words) - 02:29, 19 September 2015
• Team:China Tongji/Achivement
  ..., vulval muscles of the hermaphrodite and the diagonal muscles of the male tail." (from Wormatlas) Since we want to control the C.elegans' movement. We dec ..., vulval muscles of the hermaphrodite and the diagonal muscles of the male tail. “(from Wormatlas) Since we want to control the C.elegans' movement. We d
  67 KB (9,329 words) - 18:26, 15 October 2015
• Team:British Columbia/slide/jqueryanythingslider
  ...ail the list with 'visible' number of items, top has the last section, and tail has the first
  43 KB (5,464 words) - 04:30, 14 July 2015
• Team:Birkbeck/Conclusion
  <p>The short tail fibre (<i>stf</i>) gene was successfully reconstructed with <i>ORF-401</i> <p>The tail fibre assembly (tfa) protein acts as a molecular chaperone to stf. The clon
  3 KB (491 words) - 02:23, 19 September 2015
• Team:Paris Bettencourt/Notebook/VitaminA
  ...ock we received, with only 3 G/C). Also our 3' primer actually contained a tail that had 30 bp in common with the gBlock vA-2, for the Gibson Assembly. Wit <br>So we ordered a new 3' primer, with a tail that put back the missing 16 bp.
  58 KB (9,187 words) - 02:29, 19 September 2015
• Team:Paris Bettencourt/Notebook/Differentiationyeast
  <p>Conclusion: Use 1B : 160 (Leu2 tail F) + BB1 Use H1: His3F + 163 (Leu2 tail R) Try PCR cleanup them and follow with Gibson assembly.</p>
  38 KB (6,291 words) - 22:15, 18 September 2015
• Team:Toulouse/Experiments
  ...ucleotide must be the 20 last nucleotides of the previous fragment and the tail of the 3’oligonucleotide must be the 20 first nucleotides of the next fra
  48 KB (7,109 words) - 22:16, 18 September 2015
• Team:ATOMS-Turkiye/Project/Ulcer
  ...d by combining the N-terminal tail of PomB with the periplasmic C-terminal tail of MotB. . In order to understand how effective this protein worked, many e
  128 KB (20,011 words) - 03:42, 5 October 2015
• Team:ATOMS-Turkiye/Project/Ulcer1
  ...d by combining the N-terminal tail of PomB with the periplasmic C-terminal tail of MotB. . In order to understand how effective this protein worked, many e
  130 KB (20,222 words) - 09:29, 5 October 2015
• Team:ATOMS-Turkiye/Project/Ulcer2
  ...d by combining the N-terminal tail of PomB with the periplasmic C-terminal tail of MotB. . In order to understand how effective this protein worked, many e
  130 KB (20,395 words) - 03:27, 5 October 2015
• Team:Exeter/Design
  ...major changes. We learned that blood tests in cattle take place behind the tail, as opposed to the current skin test which takes place in the neck. Accordi ...that bovine blood tests are usually drawn from a prominent vein behind the tail of the cow, and that this kind of test has many benefits over the cumbersom
  23 KB (3,988 words) - 03:05, 19 September 2015
• Team:SJTU-BioX-Shanghai/Composite Part
  ...cpcG2-HR are approximately 370bp and 570bp respectively. The number at the tail of the sample represents our number for the colonies of transformant. ...cpcG2-HR are approximately 370bp and 570bp respectively. The number at the tail of the sample represents our number for the colonies of transformant.
  8 KB (1,146 words) - 03:41, 19 September 2015
• NJU-China-project/result.html
  ...<h2>2.3 GFP levels in the brains of GFP transgenic mice decrease after tail vein injection of a plasmid that expresses GFP siRNA</h2> ...) into RVG exosomes and injected them into GFP-transgenic mice through the tail vein. Then, the GFP levels in various tissues were determined by measuring
  33 KB (4,607 words) - 18:52, 18 September 2015
• Team:DTU-Denmark/Project/Intein
  ...protein - or in our case a short cyclic&nbsp;peptide cyclized from head-to-tail.</p> ...yclic peptides. CyBase contains&nbsp;818 entries&nbsp;of ribosomal head-to-tail cyclic&nbsp;peptides from 105 different species [1]. The&nbsp;peptides vary
  32 KB (3,737 words) - 03:59, 19 September 2015
• Team:DTU-Denmark/Project/Inteins
  ...protein - or in our case a short cyclic&nbsp;peptide cyclized from head-to-tail.</p> ...yclic peptides. CyBase contains&nbsp;818 entries&nbsp;of ribosomal head-to-tail cyclic&nbsp;peptides from 105 different species [1]. The&nbsp;peptides vary
  32 KB (3,726 words) - 15:12, 9 November 2015
• Revision history of "Team:Reading/Team"
  {{Tail}}
  4 KB (500 words) - 01:29, 13 July 2015
• Team:METU Turkey modeling
  <p>TetR+LVA: (C0040) tetracycline repressor modified with LVA tail for rapid degradation of the protein and faster fall time for the emission.
  9 KB (1,418 words) - 20:40, 11 September 2015
• Team:TU Delft/Description
  ...biofilm. To do so, we designed different biobricks that contain a peptide tail attached to the sequence of the biofilm protein CsgA that provides a specif
  78 KB (11,666 words) - 14:04, 19 November 2015
• Stylesheet.css
  .tail {
  96 B (11 words) - 12:39, 8 June 2015
• Team:Dundee/caicai
  <p>Towards the tail end of our project we gained a spot on BBC Radio Scotland and on Scottish T
  13 KB (1,791 words) - 01:00, 18 September 2015
• Team:Birkbeck/Experiments
  <p>For the assembly of the bacteriophage Lambda tail fibre protein (stf gene), our initial step was the design of ORF314. This s
  20 KB (3,069 words) - 16:41, 16 October 2015
• Team:WashU StLouis/js/backbone-min.js
  ...voke:0,max:3,min:3,toArray:1,size:1,first:3,head:3,take:3,initial:3,rest:3,tail:3,drop:3,last:3,without:0,difference:0,indexOf:3,shuffle:1,lastIndexOf:3,is
  22 KB (4,295 words) - 04:43, 7 July 2015
• Team:Dundee/Practices
  <p>Towards the tail end of our project we gained a spot on BBC Radio Scotland and on Scottish T
  16 KB (2,281 words) - 23:24, 18 September 2015
• Team:China Tongji/Project
  ..., vulval muscles of the hermaphrodite and the diagonal muscles of the male tail(from Wormatlas).
  107 KB (14,954 words) - 19:51, 18 September 2015
• Team:Santa Clara/Safety
  {{Santa_Clara/Tail}}
  3 KB (437 words) - 03:21, 19 September 2015
• Team:Paris Bettencourt/Notebook/VitaminB2
  ...alculator indicated that for the binding of the two primers (without their tail), an annealing temperature of 50°C is required. So we tried with 8 differe
  149 KB (21,246 words) - 02:36, 19 September 2015
• Team:EPF Lausanne/Notebook/Yeast
  ...Addgene. Overlaps for Gibson assembly were added by PCR. A synthetic polyA tail followed by a part of the Hammerhead ribozyme sequence was included in the
  33 KB (4,232 words) - 03:57, 19 September 2015
• Team:ETH Zurich/Parts
  <td>cI repressor from <i>E. coli</i> phage lambda modified with an LVA tail for rapid degradation of the protein</td>
  15 KB (2,521 words) - 03:35, 19 September 2015
• Team:Utah State/Description
  ...rred to as the capsid, often polyhedral in shape, and can be attached to a tail with fibers involved in attachment to specific bacterial surfaces [3,4]. Ph
  27 KB (4,010 words) - 03:53, 19 September 2015
• Team:Santa Clara/Parts
  {{Santa_Clara/Tail}}
  2 KB (195 words) - 19:35, 18 September 2015
• Team:Santa Clara/Notebook
  {{Santa_Clara/Tail}}
  35 KB (5,274 words) - 03:16, 19 September 2015
• Team:Dundee/Media
  <p>Towards the tail end of our project we gained a spot on BBC Radio Scotland and on Scottish T
  19 KB (2,748 words) - 13:42, 18 September 2015
• Team:Santa Clara/The Problem
  {{Santa_Clara/Tail}}
  2 KB (339 words) - 05:53, 15 September 2015
• Team:Birkbeck/BioBrickProtocols
  <p>For the assembly of the bacteriophage Lambda tail fibre protein (stf gene), our initial step was the design of ORF314. This s
  5 KB (767 words) - 20:38, 18 September 2015
• Team:Santa Clara
  {{Santa_Clara/Tail}}
  4 KB (565 words) - 03:28, 19 September 2015
• Team:FAU Erlangen/Tour22
  ...ere are three different models to explain regulation via N-terminal histon tail acetylation. </p>
  8 KB (1,086 words) - 03:25, 19 September 2015
• Team:Santa Clara/Policy
  {{Santa_Clara/Tail}}
  599 B (77 words) - 19:28, 18 September 2015
• Team:Santa Clara/Protocols
  {{Santa_Clara/Tail}}
  26 KB (2,428 words) - 03:18, 19 September 2015
• Team:Santa Clara/Results
  {{Santa_Clara/Tail}}
  5 KB (852 words) - 03:59, 19 September 2015
• Team:Santa Clara/Team
  {{Santa_Clara/Tail}}
  6 KB (699 words) - 03:30, 19 September 2015
• Team:Vilnius-Lithuania/Results
  ...slation levels of cI protein (weak RBS) combined with its instability (LVA tail) and its nature of a dominant repressor. These effects combined might lead
  17 KB (2,442 words) - 19:12, 1 October 2015
• Team:NTU-LIHPAO-Taiwan/Project/Overview
  ...t peptide that can restrain our appetite. Because the peptide’s head and tail are both amino acid, tyrosine (Y), it is named peptide YY (PYY). PYY has tw
  25 KB (3,185 words) - 04:50, 8 September 2015
• Team:Aix-Marseille/Results
  ...="font-family:Courier New">To improve purification process, a 10-histidine tail (Bba_K525998) has been added in C-term extremity of the cytochrome c. Durin
  40 KB (5,373 words) - 15:09, 6 October 2015
• Team:SZMS 15 Shenzhen/project
  ...romoter R0010, causing the end to the expression C0051. Because of the LVA tail, the remaining C0051 will be degraded, With the lack of C0051, the promoter
  8 KB (1,159 words) - 00:54, 19 September 2015
• Team:Freiburg/Labjournals/SurChem/March
  ..."col1">All 5 spots could be detected but the bBSA-spots had a strong comet tail. This might be due to proteins adhering to the surface during the washing s
  14 KB (2,205 words) - 08:08, 10 September 2015
• Team:Glasgow/Project/Overview/Repressors
  ...p://parts.igem.org/Part:BBa_C0040">C0040</a> has been modified with an LVA tail for rapid degradation while PhlF has not, meaning the fold repression is no
  28 KB (3,654 words) - 03:29, 21 November 2015
• Team:Santa Clara/Project
  {{Santa_Clara/Tail}}
  2 KB (268 words) - 02:03, 14 September 2015
• Team:Santa Clara/NatureSolution
  {{Santa_Clara/Tail}}
  4 KB (576 words) - 19:02, 18 September 2015
• Team:Santa Clara/OurDefenseSystem
  {{Santa_Clara/Tail}}
  3 KB (494 words) - 19:05, 18 September 2015
• Team:Santa Clara/FutureProspects
  {{Santa_Clara/Tail}}
  4 KB (667 words) - 03:52, 19 September 2015
• Team:Santa Clara/TheProblem
  {{Santa_Clara/Tail}}
  3 KB (386 words) - 19:01, 18 September 2015
• Team:Santa Clara/Collaboration
  {{Santa_Clara/Tail}}
  1 KB (174 words) - 03:14, 19 September 2015
• Team:Heidelberg/project/cf
  ...ng it difficult to observe. The cleaved, mRNA lacking the protective polyA-tail would be rapidly degraded, leading to the observed drop in expression. To d
  37 KB (5,500 words) - 15:26, 20 November 2015
• Team:XJTLU-CHINA/Mideling
  ...ral decay rate, the degradation of LuxI was accelerated by introducing LVA tail in marine cells. </p>
  17 KB (1,794 words) - 07:50, 17 September 2015
• Team:XJTLU-CHINA/youshalnotcast
  ...ral decay rate, the degradation of LuxI was accelerated by introducing LVA tail in marine cells. </p>
  23 KB (2,344 words) - 09:00, 17 September 2015
• Team:Bordeaux/Yeast results
  ...tify"> We have designed two types of primers that differ in their floating tail. Each of the primer present floating tails with restriction sequences.
  7 KB (1,075 words) - 02:47, 19 September 2015
• Team:SJTU-BioX-Shanghai/Transport Module
  ...dark-HR and PcpcG2-HR are 370bp and 570bp respectively. The numbers at the tail of the sample names are the numbers of our transformants.
  7 KB (949 words) - 03:38, 19 September 2015
• Team:XJTLU-CHINA/Notebook
  ..., 8, 10, 13 with terminator and connected blue chromoprotein with LVA, AAV tail and terminator as well as the J23100 promoter. As always, we introduced the
  78 KB (7,104 words) - 16:15, 4 October 2015
• Team:XJTLU-CHINA/Modeling
  ...ral decay rate, the degradation of LuxI was accelerated by introducing LVA tail in marine cells. </p>
  36 KB (3,193 words) - 16:16, 4 October 2015
• Team:NJAU China/Experiments
  <p>Materials: PCR product adding A tail, pMD®18-T Vector,<br />System (10μl):</p>
  6 KB (950 words) - 02:21, 19 September 2015
• NJU-China-parts.html
  ...) into RVG exosomes and injected them into GFP-transgenic mice through the tail
  35 KB (5,029 words) - 19:30, 18 September 2015
• Team:UI Indonesia/Parts
  ...e mobility of sperm cell. Subtilosin A break down membrane plasma and coil tail of sperm cell. This protein also known that it doesn’t affect population
  12 KB (1,631 words) - 03:54, 19 September 2015
• Team:Santa Clara/Attributions
  {{Santa_Clara/Tail}}
  6 KB (959 words) - 03:36, 19 September 2015
• Team:Santa Clara/Achievements
  {{Santa_Clara/Tail}}
  6 KB (847 words) - 03:15, 19 September 2015
• Team:NJAU China/Results
  ...ffice Excel to figure out the consequence of paired two-sample t-test (one tail).<br />
  17 KB (2,676 words) - 03:53, 19 September 2015
• Team:Santa Clara/Practices
  {{Santa_Clara/Tail}}
  19 KB (2,972 words) - 03:57, 19 September 2015
• Team:UCLA/Project
  ...non-specificity in primer binding. While other techniques such as head-to-tail assembly or concatemerization have been developed to facilitate spider silk
  11 KB (1,692 words) - 00:03, 2 October 2015