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  • Giant Jamboree/Projects
    ...s ></A><BR><BR>< Gastric ulcer is a disease that caused by a gram negative bacteria called H. Pylori. As far as we know, ½ of human population is effected wit ...cal engineering bacteria, connected with the aptamers, to make engineering bacteria able to detect the macromolecules, thus providing biological solutions in t
    271 KB (42,018 words) - 20:59, 14 September 2015
  • Team:Oxford/Blog
    ...AlgiBeads</a>. These represent an additional method of containment for our bacteria and, along with the <a class="definition" title="catheter" data-content="A and kill switches, allow us to ensure the safety of our bacteria as a therapeutic agent.
    107 KB (13,740 words) - 02:51, 19 September 2015
  • Team:Oxford/Test/Blog
    ...with the catheter and kill switches, allow us to ensure the safety of our bacteria as a therapeutic agent. ...the 1950s, around the time antibiotic use reached large scales. While some bacteria have been resistant to a given antibiotic prior to it being used as a medic
    102 KB (12,840 words) - 14:21, 12 September 2015
  • Team:ATOMS-Turkiye/Project/Ulcer
    ...of all other proteins. By overexpressing this protein, we aim to have our bacteria resistant enough to live in gastric juice for a while.</p> ...oli so that It can penetrate the stomach mucus layer, where our pathogenic bacteria, H.pylori, resides.</p>
    128 KB (20,011 words) - 03:42, 5 October 2015
  • Team:ATOMS-Turkiye/Project/Ulcer1
    ...of all other proteins. By overexpressing this protein, we aim to have our bacteria resistant enough to live in gastric juice for a while.</p> ...oli so that It can penetrate the stomach mucus layer, where our pathogenic bacteria, H.pylori, resides.</p>
    130 KB (20,222 words) - 09:29, 5 October 2015
  • Team:ATOMS-Turkiye/Project/Ulcer2
    ...of all other proteins. By overexpressing this protein, we aim to have our bacteria resistant enough to live in gastric juice for a while.</p> ...oli so that It can penetrate the stomach mucus layer, where our pathogenic bacteria, H.pylori, resides.</p>
    130 KB (20,395 words) - 03:27, 5 October 2015
  • Team:WLC-Milwaukee/Project
    <b>Problem:</b> Bacteria are becoming increasingly resistant to antibiotics at an alarming rate. ...iawiki/2015/d/d3/WLC-DropArrow.png" width="33" height="21">Disease Causing Bacteria</b></font></h1></label>
    54 KB (8,117 words) - 03:20, 19 September 2015
  • Team:USTC/Results
    won't severely affect the growth of bacteria, at least it won't significantly inhibit bacteria growth and become a kill switch.</p>
    79 KB (7,338 words) - 16:20, 14 October 2015
  • Team:Wellesley TheTech/Team/Notebooks
    ...Children, and the viruses stayed inside the bacteria and did not leave the bacteria. ...e) mitosis and the asexual replication of bacteria. It copies the way that bacteria duplicates (increases to double its size and then divides into two at its m
    99 KB (17,327 words) - 03:41, 19 September 2015
  • Team:Oxford/Design
    ...our project. The most effective approach would probably be to deliver our bacteria directly through the catheter into the bladder. However, we found that the ...The ability to disperse biofilms formed by multidrug-resistant bacteria adds a major new weapon to the limited arsenal of therapies available today
    31 KB (3,744 words) - 11:05, 20 November 2015
  • Team:Vilnius-Lithuania/Labjournal
    <td>Bacteria inoculation (4 minipreps).</td> <td>Bacteria with P14</td>
    48 KB (6,078 words) - 03:56, 19 September 2015
  • Team:TU Delft/Practices
    <p class="lead">The biofilm industry is generally regulated with respect to bacteria present in the biofilm. Thus, diverse regulations may apply. A special cas ...experiments do not involve any human or other living test subjects (except bacteria). In this sense, it is easier and cheaper for industry to work with biofilm
    139 KB (20,165 words) - 22:15, 17 September 2015
  • Team:Czech Republic/Notebook
    ...ccessful Gibson assembly. ([[Team:Czech_Republic/Protocols#Transformation_(bacteria)|Transformation]], [[Team:Czech_Republic/Protocols#Colony_PCR|Colony PCR]], ...#Gibson|Gibson assembly]], [[Team:Czech_Republic/Protocols#Transformation_(bacteria)|Transformation]], [[Team:Czech_Republic/Protocols#Colony_PCR|Colony PCR]],
    55 KB (7,346 words) - 03:19, 19 September 2015
  • Team:Oxford/Practices
    ...ackle <a class="definition" title="antimicrobial resistance" data-content="Bacteria adapt to their surroundings, and can become resistant to powerful antibioti ...15.igem.org/Team:Oxford/Project">project</a>, we are developing the use of bacteria as living therapeutics to provide an alternative to administering antibioti
    59 KB (7,066 words) - 02:59, 19 September 2015
  • Team:TAS Taipei/wetlab
    ...(Figure 6); however, under blue light conditions, it can be seen that only bacteria incubated at 37⁰C actually glowed (Figure 7). This is evidence that the t ...</i> Carrying Temperature-Sensitive Promoter and GFP Generator Device:</b> Bacteria grew in all tested temperatures (shown here: 30⁰C, 32⁰C, 35⁰C, and 37
    44 KB (6,376 words) - 02:45, 19 September 2015
  • Team:UChile-OpenBio/Modelling
    ...cellular communication between “Lactadora” bacteria and “PLAdora” bacteria, and to analyze the situation (see Figure 1).<p/> ...ing lactate without regulation and the green producing PLA; C Two bacteria growing together, the orange producing lactate and the green producing PLA, without
    121 KB (18,837 words) - 13:54, 18 September 2015
  • Team:UChile-OpenBio/Modeling
    ...cellular communication between “Lactadora” bacteria and “PLAdora” bacteria, and to analyze the situation (see Figure 1).</p> ...ing lactate without regulation and the green producing PLA; C Two bacteria growing together, the orange producing lactate and the green producing PLA, without
    211 KB (20,494 words) - 18:35, 14 November 2015
  • Team:Oxford/Description
    ..."Years from now we will treat most infections with bacteria and not antibiotics." ...major public health concern in developed countries. This is largely due to growing antibiotic resistance.
    41 KB (4,920 words) - 11:29, 20 November 2015
  • Team:Oxford/testtest
    ..."Years from now we will treat most infections with bacteria and not antibiotics." ...major public health concern in developed countries. This is largely due to growing antibiotic resistance.
    41 KB (4,891 words) - 10:02, 17 November 2015
  • Team:SPSingapore/home
    ...rsity of Illinois-Chicago. Her laboratory studies two-component systems in bacteria that control gene expression at a single cell and nanometer level.</p></td> ...in studying silent genetic systems in enterics to decipher the behavior of bacteria under various environmental challenges. She obtained her doctorate from the
    145 KB (23,871 words) - 16:19, 8 September 2015
  • Team:Utah State/Description
    ...d research of this sort to creating phage resistant strains of lactic acid bacteria.</p> ...eria are able to fight against the infection, a kill switch will cause the bacteria to lyse before the phage is able to fully form, preventing further damage t
    27 KB (4,010 words) - 03:53, 19 September 2015
  • Team:elan vital korea/Background
    Bacteria acquiring resistance to antibiotics pose serious health problem globally. F Threats of Antibiotics-Resistant Bacteria
    25 KB (3,338 words) - 14:50, 18 September 2015
  • Team:Elan Vital Korea/Background
    Bacteria acquiring resistance to antibiotics pose serious health problem globally. F Threats of Antibiotics-Resistant Bacteria
    25 KB (3,338 words) - 23:24, 18 September 2015
  • Team:LZU-China/chz project
    ...nverts biomass energy directly into electricity. This can be achieved when bacteria switch from the natural electron acceptor, such as oxygen or nitrate, to an <p>Substrate is metabolized by bacteria, which transfer the gained electrons to the anode. This can occur either di
    110 KB (13,643 words) - 19:50, 17 September 2015
  • Team:LZU-China/project
    ...nverts biomass energy directly into electricity. This can be achieved when bacteria switch from the natural electron acceptor, such as oxygen or nitrate, to an <p>Substrate is metabolized by bacteria, which transfer the gained electrons to the anode. This can occur either di
    121 KB (14,975 words) - 03:27, 19 September 2015
  • Team:Elan Vital Korea/Description
    <p class="content-txt m20">Bacteria that acquire resistance to antibiotics pose a serious health problem global <h1 class="sub-title align-c m80">Threats of Antibiotics-Resistant Bacteria</h1>
    14 KB (2,086 words) - 14:51, 18 September 2015
  • Team:LZU-China/chz project2
    ...nverts biomass energy directly into electricity. This can be achieved when bacteria switch from the natural electron acceptor, such as oxygen or nitrate, to an ...he working principle of a microbial fuel cell. Substrate is metabolized by bacteria, which transfer the gained electrons to the anode. This can occur either di
    88 KB (10,264 words) - 22:18, 9 September 2015
  • Team:Oxford/Description-backup
    ..."Years from now we will treat most infections with bacteria and not antibiotics." ...major public health concern in developed countries. This is largely due to growing antibiotic resistance.
    52 KB (5,967 words) - 10:05, 17 November 2015
  • Team:Oxford/Test/Design1
    ...during the beginning stages of our project. The approach of delivering our bacteria directly through the catheter into the bladder would probably be the most e ...could potentially be used to a large range of different pipes to tackle a growing world problem with biofilms we decide to focus on a medical application for
    15 KB (2,035 words) - 09:43, 7 September 2015
  • Team:TAS Taipei/Design
    ...most socially acceptable method of drug delivery: a topical solution where bacteria (our chassis) is prevented from entering into the body, and the only thing ...r (yellow interior). The pores of the membrane are small enough to prevent bacteria, but allow our final GzmB inhibitor, to pass through.</figcaption>
    25 KB (3,462 words) - 02:43, 19 September 2015
  • Team:Paris Saclay/Description
    ...</b>. <i>E. coli</i> is often chosen because of it is easy to handle, fast growing and the high level of knowledge is available for this organism.<br> ...uld have allowed us to evaluate easily the number of living <i>E. coli</i> bacteria in our samples. However, using such strain had also several drawbacks.
    49 KB (7,759 words) - 03:58, 19 September 2015
  • Team:Toulouse/Results
    ...rt of the results, we also showed that this 4% concentration is lethal for bacteria. We therefore decided to aim for a minimal concentration of 0.00001 % butyr We investigated if the bacteria could grow inside a small bag of TPX®. Thus, the strain <i>E. coli</i> BW
    55 KB (8,373 words) - 03:00, 19 September 2015
  • Team:Oxford/Test/Design
    ...during the beginning stages of our project. The approach of delivering our bacteria directly through the catheter into the bladder would probably be the most e ...could potentially be used to a large range of different pipes to tackle a growing world problem with biofilms we decide to focus on a medical application for
    14 KB (1,894 words) - 13:13, 15 September 2015
  • Team:Glasgow/Practices/Bedtimestories
    “You grow bacteria inside its tummy,” he replied. ...ven see them unless you have a special microscope. There are good kinds of bacteria and nasty kinds, though. Do you remember how I always ask you to wash your
    23 KB (3,106 words) - 03:32, 21 November 2015
  • Team:Oxford/Delivery
    ...resses and attack from a host’s immune system (in the case of pathogenic bacteria).">biofilm</a> also forms on the outside of the catheter so in our design w ...could potentially be used to a large range of different pipes to tackle a growing world problem with biofilms we decide to focus on a medical application for
    17 KB (2,246 words) - 11:01, 17 September 2015
  • Team:Oxford/Test/Practices
    ...2015.igem.org/Team:Oxford/Design">system</a>, we are developing the use of bacteria as living therapeutics to provide an alternative to administering antibioti ...ABR and synthetic biology; and our tools are based around using engineered bacteria as therapeutic agents. The targeted nature of our solution is achieved by b
    28 KB (3,599 words) - 11:01, 17 September 2015
  • Team:Glasgow/Design
    ...the bacteria. It is transparent enough to allow the UV-A rays to reach the bacteria, and for their bioluminescence to shine out. ...roth to drop out as easily as possible. Any crevices would allow broth and bacteria to remain and not be flushed out, which could cause problems.</p>
    17 KB (2,118 words) - 03:31, 21 November 2015
  • Team:UI Indonesia/pnp
    ...the consument target, most of the respondents agreed if we use engineered bacteria as the active material for our contraceptives. They agreed with our inovati ...re, our products are also used by women. We decide to do this because the bacteria’s biological properties is suitable with the vagina condition. Some peopl
    78 KB (12,133 words) - 20:22, 18 September 2015
  • Team:Danzi Kesh 8/Notebook
    ...ass="list-item">Preparation of growth platforms (substrates) for&nbsp; the bacteria -&nbsp; The first platform with two ampicillin and the second one with chlo ...-item">Performing transformation using the Heat Shock method to the E.coli bacteria competents - Type DH5α.</li>
    49 KB (6,726 words) - 09:36, 18 September 2015
  • Team:Glasgow/Practices
    ...believed their children were already exposed to a lot of much more harmful bacteria in every day life, so more education for younger kids was definitely needed ...ntainer themselves – which led us to design the removable handle for the bacteria’s release. To read more about this, see our <a href="https://2015.igem.or
    27 KB (3,634 words) - 17:24, 14 November 2015
  • Team:WLC-Milwaukee/Practices
    ...and learned the important conditions necessary for doing so. We then took bacteria and isolated their DNA by performing a “mini prep”. </p> ...to understand RFP, which we used in the lab yesterday with our glowing red bacteria! The small scale science that we are performing in the lab this week really
    41 KB (6,551 words) - 03:21, 19 September 2015
  • Team:Oxford/Notebook
    <h4>Growth and Culture of Bacteria </h4> ...The inversion in step 6 needs to be done gently so that genomic DNA of the bacteria are not extracted along with the desired plasmid DNA.</li>
    530 KB (37,526 words) - 03:47, 19 September 2015
  • Team:Oxford/Protocols
    ...D<sub>600nm</sub> reaches 0.4-0.6. It is good to stop at OD = 0.35, as the bacteria are now replicating exponentially i.e. will only take 20 more minutes until <h3>1.6 Growth and Culture of Bacteria</h3>
    80 KB (8,137 words) - 14:47, 14 November 2015
  • Team:Oxford/Test/Notebook
    <h4>Growth and Culture of Bacteria </h4> ...The inversion in step 6 needs to be done gently so that genomic DNA of the bacteria are not extracted along with the desired plasmid DNA.</li>
    500 KB (35,542 words) - 16:53, 18 September 2015
  • Team:DTU-Denmark/Project/MAGE
    ...myE::beta-neoR </em>and <em>∆amyE::GP35-neoR </em>was shown to be faster growing than the wild type strain.</p> ...e faster growing than the wild type. The mutS::beta was shown to be slower growing than the wild type, this mutant is the one that is best at recombineering.
    80 KB (10,239 words) - 15:12, 9 November 2015
  • Team:Yale/notebook
    <h2>Week 5</h2><a href="#" data-reveal-id="week6">Growing and Amplifying</a> ...which the technique is proven to work, to Cyanobacteria and Sinorhizobium bacteria.</p>
    48 KB (7,180 words) - 03:26, 19 September 2015
  • Team:WLC-Milwaukee/HumanPractices
    ...and learned the important conditions necessary for doing so. We then took bacteria and isolated their DNA by performing a “mini prep”. ...to understand RFP, which we used in the lab yesterday with our glowing red bacteria! The small scale science that we are performing in the lab this week really
    24 KB (3,835 words) - 06:11, 15 September 2015
  • Team:SPSingapore/Anaerobic Promoter
    ...ng pnirB-invasin can invade mammalian cells under anaerobic conditions and bacteria cells transformed with plasmids encoding pnirB-invasin-listeriolysin can in ...sion assay, where results are represented in colony-forming units (CFU) of bacteria successful in invading mammalian cells (Figure 7). Our results show that BL
    30 KB (4,339 words) - 04:21, 17 November 2015
  • Team:Vilnius-Lithuania/Application
    ...ed to theoretically incorporate our system in their genetically engineered bacteria.</p> ...in the environmet and live bees, it’s easy to imagine that this kind of bacteria can spread in the dark and get into other species.</p>
    5 KB (743 words) - 10:21, 18 September 2015
  • Team:Central Calgary/project
    ...ars itself out via a natural, frequently-occurring cycle, meaning that the bacteria would naturally be cleared out as well. Due to this evidence, a kill switch ...lactic acid are needed to inhibit angiogenesis. The assay would be done by growing cultures with varying levels of lactic acid and lysing them on an in vitro
    18 KB (2,642 words) - 20:39, 24 December 2015
  • Team:UCL/Entrepreneurship
    ...rding to a customer’s needs (personalisation) we’re able to leverage a growing body of research to a friendly bacteria, which have been shown to interact with your gut-brain axis, to make you ha
    55 KB (8,255 words) - 15:53, 22 October 2015
  • Team:Carnegie Mellon/Notebook
    The colonies on the plate with the T7 YFP without the sites was growing extremely slowly so it was left in the 37C incubator for another night. For ...cultures from previous cultures, with the exception of the YFP transformed bacteria, which was taken from a plate created 5/27/2015.</li>
    72 KB (10,158 words) - 20:59, 20 November 2015
  • Revision history of "Editing Team:Oxford/Protocols"
    ...D<sub>600nm</sub> reaches 0.4-0.6. It is good to stop at OD = 0.35, as the bacteria are now replicating exponentially i.e. will only take 20 more minutes until <h3>1.6 Growth and Culture of Bacteria</h3>
    75 KB (7,507 words) - 09:40, 15 September 2015
  • Team:Oxford/Test/Protocols
    ...D<sub>600nm</sub> reaches 0.4-0.6. It is good to stop at OD = 0.35, as the bacteria are now replicating exponentially i.e. will only take 20 more minutes until <h3>1.6 Growth and Culture of Bacteria</h3>
    77 KB (7,823 words) - 20:37, 16 September 2015
  • Team:UCSC/Logs
    ...;">Multiple sequence alignment of conserved proteins conserved in Archaea, Bacteria, and Eukaryotes </span></li> ...an style="font-weight: 400;">Prepared 500 mL of LB agar in preparation for growing transformed </span><em><span style="font-weight: 400;">E. coli </span></em>
    123 KB (16,952 words) - 23:53, 17 September 2015
  • Team:Hangzhou-H14Z
    ...the constant volume of 50mL. Use 0.22μm strainer to filter out the other bacteria, then put into refrigerator of -20°C. </p> ...0;&#160;&#160;&#160;&#160;&#160;(3) Pick bacteria into small colonies from growing strains on the cultivation board, which has been configured with a medium,
    101 KB (12,474 words) - 01:53, 19 September 2015
  • Team:Kent/Practices
    ...percentage of the current electronics are not renewable and contribute to growing volumes of e-waste. One of the components is copper and other non-renewable <b>4) The charging stations that we proposed would contain bacteria to produce the energy. What are your views on the matter?</b> <br><br>
    48 KB (8,114 words) - 00:45, 19 September 2015
  • Team:Reading/Practices
    ...i/fimbriae, so if it say picked up the hyperpilation modification from our bacteria, this could make the pathogen more virulent.</p> ...rms of having a constant light and nutrient supply. It also means that the bacteria have a longer effective life when in the cell as they will not be consuming
    19 KB (3,060 words) - 21:10, 16 September 2015
  • Team:Hangzhou-H14Z/Attributions
    ...the constant volume of 50mL. Use 0.22μm strainer to filter out the other bacteria, then put into refrigerator of -20°C. </p> ...0;&#160;&#160;&#160;&#160;&#160;(3) Pick bacteria into small colonies from growing strains on the cultivation board, which has been configured with a medium,
    101 KB (12,459 words) - 18:45, 18 September 2015
  • Team:Hangzhou-H14Z/Parts
    ...the constant volume of 50mL. Use 0.22μm strainer to filter out the other bacteria, then put into refrigerator of -20°C. </p> ...0;&#160;&#160;&#160;&#160;&#160;(3) Pick bacteria into small colonies from growing strains on the cultivation board, which has been configured with a medium,
    102 KB (12,478 words) - 01:48, 19 September 2015
  • Team:Hangzhou-H14Z/Collaborations
    ...the constant volume of 50mL. Use 0.22μm strainer to filter out the other bacteria, then put into refrigerator of -20°C. </p> ...0;&#160;&#160;&#160;&#160;&#160;(3) Pick bacteria into small colonies from growing strains on the cultivation board, which has been configured with a medium,
    101 KB (12,459 words) - 18:44, 18 September 2015
  • Team:Hangzhou-H14Z/Project
    ...the constant volume of 50mL. Use 0.22μm strainer to filter out the other bacteria, then put into refrigerator of -20°C. </p> ...0;&#160;&#160;&#160;&#160;&#160;(3) Pick bacteria into small colonies from growing strains on the cultivation board, which has been configured with a medium,
    101 KB (12,459 words) - 18:58, 18 September 2015
  • Team:Hangzhou-H14Z/Practices
    ...the constant volume of 50mL. Use 0.22μm strainer to filter out the other bacteria, then put into refrigerator of -20°C. </p> ...0;&#160;&#160;&#160;&#160;&#160;(3) Pick bacteria into small colonies from growing strains on the cultivation board, which has been configured with a medium,
    101 KB (12,459 words) - 18:46, 18 September 2015
  • Team:Hangzhou-H14Z/Intro
    ...the constant volume of 50mL. Use 0.22μm strainer to filter out the other bacteria, then put into refrigerator of -20°C. </p> ...0;&#160;&#160;&#160;&#160;&#160;(3) Pick bacteria into small colonies from growing strains on the cultivation board, which has been configured with a medium,
    101 KB (12,459 words) - 18:57, 18 September 2015
  • Team:Hangzhou-H14Z/Team
    ...the constant volume of 50mL. Use 0.22μm strainer to filter out the other bacteria, then put into refrigerator of -20°C. </p> ...0;&#160;&#160;&#160;&#160;&#160;(3) Pick bacteria into small colonies from growing strains on the cultivation board, which has been configured with a medium,
    101 KB (12,459 words) - 18:59, 18 September 2015
  • Team:Hangzhou-H14Z/Modeling
    ...the constant volume of 50mL. Use 0.22μm strainer to filter out the other bacteria, then put into refrigerator of -20°C. </p> ...0;&#160;&#160;&#160;&#160;&#160;(3) Pick bacteria into small colonies from growing strains on the cultivation board, which has been configured with a medium,
    101 KB (12,459 words) - 19:06, 18 September 2015
  • Team:Lethbridge HS/Notebook
    ...4 hours, all mites were found to be dead. Pupae showed some signs of black bacteria growth or browning on body. Will need to repeat or find another method of c ...<p>Objective:Determine if the concentration of oxalic acid that kills bacteria will kill bees.<br><br>
    159 KB (13,078 words) - 02:08, 19 September 2015
  • Team:OUC-China/Note Book/Lab Journal
    ...resis, then they were transformed into Top 10 cells. There are no colonies growing on the plate. ...nd transformation were down by Cun Wei and Zhuo Pan. There are no colonies growing on the plate.<BR>Cun Wei repeated agarose gel electrophoresis and ligation
    78 KB (9,885 words) - 18:42, 18 September 2015
  • Team:HKUST-Rice/TXTL
    ...reading of what nutrients are readily available from the perspective of a growing plant.</p> ...n-fixing, denitrifying, phosphate-solubilizing, and potassium-solubilizing bacteria have been identified (sources). By hooking up our individual nutrient sens
    10 KB (1,420 words) - 23:13, 14 September 2015
  • Team:Elan Vital Korea/Team
    ...e explained by science - even the smallest and most invisible things, like bacteria and their methods of communication and infection, which our team has been f ...mwork iGEM has provided. Synthetic biology is a newly emerging yet rapidly growing branch of science, and does not exist in nature yet creates mechanisms of l
    22 KB (3,761 words) - 18:23, 18 September 2015
  • Team:UMaryland/HokSok
    <li>2. Does the Hok-Sok cassette restrict the growth of the bacteria it resides in?</li> ...ther or not the maintenance system is effective in preserving a plasmid in bacteria that is not beneficial to its survival, such as the aforementioned RFP.
    16 KB (2,547 words) - 03:57, 19 September 2015
  • Team:UNAM-CU/project
    ...aims to induce the production of insulin according to glucose levels. The bacteria are going to be contained in a modular device composed by contention, commu ...as allowing glucose levels’ information to pass through it and reach the bacteria, but this container also aims to prevent bacterial physical contact with th
    20 KB (3,210 words) - 03:53, 19 September 2015
  • Team:British Columbia/Growing
    ...to make these bacteria genetically tractable. This included culturing the bacteria on different growth media, testing methods of competence induction, and tra ...i>), the first step was to identify the optimal method of culturing either bacteria. </p>
    25 KB (3,623 words) - 02:07, 19 September 2015
  • Team:UNITN-Trento/Results/Proteorhodopsin
    ...containing the blue wavelength, activates proteorhodopsin, thus making the bacteria <strong>survive better anaerobically</strong> and produce <strong>more ATP< ...uid, washing them and autoclaving them, the bottles were ready to host our bacteria!</p>
    28 KB (3,923 words) - 17:00, 18 September 2015
  • Team:TU Delft/Notebook
    <p class="lead">Our harmless bacteria produces curli subunits in order to make an inducible biofilm. The curli is ...ith the example of KAN). Cultures were then putted at 37°C at 200 rpm for growing.</p>
    158 KB (22,659 words) - 22:52, 18 September 2015
  • Team:York/Business
    <p>The next part is collecting the phosphate from the bacteria in the bioreactor. Engineered Bioreactors have genetically modification the ...companies generating a profit for Engineered Bioreactors. In addition the bacteria can be placed back into the bioreactor ensuring low operation costs.
    30 KB (4,784 words) - 23:39, 18 September 2015
  • Team:York/Entrepreneurship
    ...for the whole marketplace. The wastewater industry is one of the fastest growing industries in the world and is estimated to be worth £61.4 billion by 2019 <p>The next part is collecting the phosphate from the bacteria in the bioreactor. Engineered Bioreactors have genetically modified the <i>
    49 KB (7,842 words) - 01:12, 19 September 2015
  • Team:Stanford-Brown/PHA
    <p>Since bacteria that naturally synthesize P(3HB) use the plastic as an energy storage mecha ...the cells can continue to grow. The rest of the population would continue growing and producing plastic. The plastic can be recovered from the media by causi
    23 KB (3,737 words) - 03:43, 19 September 2015
  • Team:NYMU-Taipei/Practices
    ...all over the world. Common diseases found in potatoes are bacterial wilt, bacteria soft rot, ring rot and late blight. Recently late blight becomes the most s <p>Growing period:<br></p>
    55 KB (8,567 words) - 23:41, 20 November 2015
  • Team:IIT Kharagpur/Project
    bacteria, yeast and moulds on your food. It's the diverse repertoire of enzymes ...es is critical for bacterial survival and interactions in natural habitat. Bacteria often take many actions, the decision of which is based upon assesment of i
    20 KB (2,690 words) - 23:32, 20 November 2015
  • Team:HKUST-Rice/Application
    ...reading of what nutrients are readily available from the perspective of a growing plant.</p> ...n-fixing, denitrifying, phosphate-solubilizing, and potassium-solubilizing bacteria have been identified (Vessey, 2003, Ngoc Diep & Ngoc Hieu, 2014). By attach
    9 KB (1,401 words) - 13:23, 18 September 2015
  • Team:Paris Bettencourt/Design
    ...ufacture a simple and practical way to distribute our engineered yeast and bacteria (See <a href="https://2015.igem.org/Team:Paris_Bettencourt/Project/Manufact <br><br>We believe that a culture of yeast and bacteria that can be grown at home and supplements
    20 KB (3,005 words) - 01:21, 21 November 2015
  • Team:elan vital korea/Members
    ...bigger competitors like humans. That’s why we have antibiotic-resistant bacteria. So, it’s very Promethean, like endless swing between victory and defeat ..., we took one step further: let a bacteria tell us if antibiotic-resistant bacteria is found in their neighborhood, using their own communication method, quoru
    24 KB (3,816 words) - 23:11, 18 September 2015
  • Team:Vilnius-Lithuania/Measurement
    ...three promoters and GFP gene, were transformed into E. coli DH5α strain. Bacteria, containing the plasmids, were grown overnight and then DNA extraction ensu ...r-left: 5px solid rgb(236,151,31); padding-left: 5px; border-bottom: none">Growing</h3>
    8 KB (1,142 words) - 10:51, 16 September 2015
  • Team:elan vital korea/members
    ...bigger competitors like humans. That’s why we have antibiotic-resistant bacteria. So, it’s very Promethean, like endless swing between victory and defeat ..., we took one step further: let a bacteria tell us if antibiotic-resistant bacteria is found in their neighborhood, using their own communication method, quoru
    24 KB (3,816 words) - 22:46, 18 September 2015
  • Team:BNU-CHINA/Safety
    ...ovement at the same time, thus preventing the difussion of our engineering bacteria to soil. ...rial colony density artificially and therefore the quantity of engineering bacteria can be maintained in a stable and controllable range.
    10 KB (1,596 words) - 20:09, 18 September 2015
  • Team:IIT Kharagpur/project
    ...chemical reactions and microbial actions most commonly by microbes such as bacteria, yeast and moulds. Microbial ...for bacterial survival and interactions in natural habitat. It can be said bacteria takes decisive action based upon assessing local cell densities.</br></br>C
    19 KB (2,501 words) - 06:29, 18 September 2015
  • Team:Elan Vital Korea/Members
    ...bigger competitors like humans. That’s why we have antibiotic-resistant bacteria. So, it’s very Promethean, like endless swing between victory and defeat ..., we took one step further: let a bacteria tell us if antibiotic-resistant bacteria is found in their neighborhood, using their own communication method, quoru
    24 KB (3,816 words) - 23:20, 18 September 2015
  • Team:CAU China/Notebook
    ...fire in the lab, how to deal with the poisonous chemical and the dangerous bacteria.</p> <p>There were many colonies growing up on the plate</p>
    7 KB (1,298 words) - 13:14, 6 September 2015
  • Team:CAU China/Experiments
    ...fire in the lab, how to deal with the poisonous chemical and the dangerous bacteria.</p> <p>There were many colonies growing up on the plate</p>
    7 KB (1,293 words) - 17:53, 17 September 2015
  • Team:Oxford/Test/Project
    ...f antimicrobial resistance. The aim of our project is to contribute to the growing body of research into providing a solution to the threat of antimicrobial r ...Although bacteria are generally thought of as causing infection, most bacteria that live inside the human body are non-pathogenic and some of them can be
    23 KB (2,734 words) - 14:15, 24 August 2015
  • Team:Paris Bettencourt/Project/Continuity
    ...allow the manufacturer to detect contamination, and check that what he is growing is exactly what he wants to grow.</li> ...ble to make a strain that fullfills its nutrient-producing functions while growing as fast as the wild type, so we found a workaround: the cells that people u
    31 KB (5,166 words) - 16:08, 15 October 2015
  • Team:IIT Kharagpur
    ...y human being.</br></br> The major challenges that mankind faces today are growing population, rapidly depleting resources and inequitable distribution of wea It is known, for quite some time now, that bacteria possess cell to cell communication capability. The communication is stimula
    17 KB (2,373 words) - 23:31, 20 November 2015
  • Team:Austin UTexas/Practices
    ...gy can lead to technologies such as bacterial photography, tumor-targeting bacteria, and biofuels (Sleight, 2010). However, in order for a genetically enhanced ...for digestive health (Ruder 2011). By ensuring that genetically modified bacteria are as stable as possible, the risks of synthetic biology therapies can be
    8 KB (1,193 words) - 03:51, 19 September 2015
  • Team:Hamilton McMaster/blog/2015-08-24
    ...ntaining growth medium for bacteria (a.k.a. this is the world in which our bacteria obtain their nutrients from). It’s a barely-clumpy powder in solid form, ...ification agent. These are made in common but disposable petri dishes. The bacteria can be spread and streaked and picked from these plates (more on this in la
    16 KB (2,764 words) - 07:33, 18 September 2015
  • Team:WLC-Milwaukee/Modeling
    ...e unable to form a complete antibiotic-efflux protein complex. As a result bacteria without a functioning <i>tolC</i> gene will show an increased sensitivity t ...project; downregulation (or stopping transcription) would likely make the bacteria hypersensistive to the antibiotics, and an upregulation could increase the
    14 KB (2,236 words) - 00:51, 19 September 2015
  • Team:Cornell/notebook
    ...was then ligated with H backbone (pSB1C3) and transformed. Had some issues growing cultures with ligations. We are excited to start cloning! ...e cultures of two strains of <i>Flavobacterium</i>, and measured growth of bacteria with spectrophotometer. In addition, daily OD readings were taken to determ
    45 KB (4,129 words) - 02:19, 19 September 2015
  • Team:Oxford/Project
    ...f antimicrobial resistance. The aim of our project is to contribute to the growing body of research into providing a solution to the threat of antimicrobial r ...Although bacteria are generally thought of as causing infection, most bacteria that live inside the human body are non-pathogenic and some of them can be
    9 KB (1,073 words) - 10:13, 21 August 2015
  • Team:Stanford-Brown/Cellulose
    ...nation - When producing cellulose in large vats, contamination by fungi or bacteria can become a major issue.</p> <li>You can also try adding an antifungal that will not act upon bacteria to the medium.
    16 KB (2,346 words) - 03:44, 19 September 2015
  • Team:Slovenia HS/Practices
    using bacteria yet in Slovenia and our project is unique in that respect, so we have decid ...pool, has to closely observe all of the factors that could influence their bacteria. The computer control system is constently measuring the speed of the mixin
    24 KB (3,713 words) - 02:19, 19 September 2015
  • Team:Slovenia HS/Testing
    ...n order to determine the most optimal medium. During the incubation, stock bacteria were grown on the standardized medium LB. </p> ...tes a constant pH, sodium sulfite serves as the inhibitor of Gram-positive bacteria and meat peptone as nutrition source. We have also tested one standardized
    15 KB (2,353 words) - 01:08, 19 September 2015
  • Team:Vilnius-Lithuania/Results
    ...RNA pol. (SP2) genes. Cas3 is then recruited to hydrolyse these genes and bacteria die. </p> ...ong>Figure 4.</strong> Cell killing proficiency test. CFUs were counted of bacteria transformed with genome targeting crRNAs (SP1 or SP2), compared to no crRNA
    17 KB (2,442 words) - 19:12, 1 October 2015
  • Team:TU Darmstadt/Project/Bio/Safety/sec2
    ...lony forming units per ml culture (CFU/ml) to compare the amount of living bacteria in the cultures grown with glucose and arabinose.</p> ...> Results of the spread plate assay used to determine the amount of CFU/ml growing on LB-agar-plates containing either glucose (red) or arabinose (black)</fig
    9 KB (1,482 words) - 21:19, 19 November 2015
  • Team:Uppsala/Naphthalene
    Some bacteria have developed enzymatic pathways capable of degrading the toxic PAHs in or <u><p>Growing <i>Pseudomonas putida</i></p></u>
    12 KB (2,026 words) - 15:06, 19 November 2015
  • Team:Cornell/overview
    ...stainable and efficient aquaculture is one of the best ways of providing a growing global population with the food it needs to thrive. The overuse of antibiot ...e a more secure, sustainable future in the fish industry and beyond to aid growing population necessary to sustain the continued increase of the human populat
    10 KB (1,522 words) - 03:32, 19 September 2015
  • Team:UCLA/Notebook/Protein Cages/2 July 2015
    ...ay be done in order to verify that the plasmid of interest is still in the bacteria that grew. Notes for growing E. coli from Qiagen website:
    7 KB (1,222 words) - 00:12, 3 July 2015
  • Team:Leicester/Notebook/Week1
    ...including ones with chloramphenicol, ready to used the next day to streak bacteria</p> ...study. The parts (Promoter and GFP) were then transformed into DH5(alpha) bacteria. This is then placed on agar plates made the day before. </p>
    38 KB (4,137 words) - 23:05, 17 September 2015
  • Team:BIT-China/project Basic circuits.html
    ...y acidic and alkaline environment. So before producing acid or alkali, the bacteria should be able to tolerate acidic or alkaline environment. Two devices are Naturally, the bacteria have developed several kinds of acid resistance system to ensure the surviv
    26 KB (3,954 words) - 10:19, 6 October 2015
  • Team:UCL/Fermentation
    ...d to test two essential assumptions of our project. The first was that our bacteria can survive a shock of pH and oxygen concentration which they would need to ...arge amounts. The latter point would be necessary in order to mass-produce bacteria commercially which is essential for our <a href="https://2015.igem.org/Team
    8 KB (1,037 words) - 19:52, 16 November 2015
  • Team:Duke/Safety
    ...ere the introduction neutral stimulus could produce a negative pressure on bacteria with antibiotic resistance via activation of a programmable cell death gene <li>The use of bacteria which possess antibiotic resistance (even if only to ampicillin or chloramp
    6 KB (895 words) - 03:12, 19 September 2015
  • TU Dresden/Safety
    ...ere contained properly, we took proper care during the disposal of exposed bacteria. </p> ...was our way of contributing to the growing issue of antibiotic resistance bacteria in the population and hopefully provides a solution for other labs with sim
    5 KB (743 words) - 07:54, 17 September 2015
  • Team:Harvard BioDesign/Collaborations
    15 mL conical tubes or glass test tubes for growing liquid cultures ...6. Shake the plate out over a tray to remove all planktonic bacteria.
    21 KB (2,830 words) - 03:33, 19 September 2015
  • Team:Paris Saclay/Team
    ...change the bacteria color depending of its mood: it would be green if the bacteria feels happy, red if it's angry, and black if it feels depressed or wistful. ...tion of a protein implicated in radioresistance in Deinococcus radiodurans bacteria</p></article>
    8 KB (1,345 words) - 09:53, 23 November 2015
  • Team:BABS UNSW Australia/overview
    ...ained on two plasmids and integrated with the pHlow system, will ensure no bacteria which leave the lab remain invasive and prevents horizontal gene transfer. ...i> K12, a small, food-grade bacteria, <i>Lactococcus lactis</i> and a slow-growing cyanobacteria, <i>Synechocystis</i> PCC6803. Each of these are exciting or
    5 KB (731 words) - 01:50, 27 October 2015
  • Team:SF Bay Area DIYBio/Notebook
    .../18988020</a></span></p><p class="c2"><span></span></p><p class="c3"><span>growing mutator strain without accumulating mutations in chromosome (see section 3.
    137 KB (23,561 words) - 02:27, 22 November 2015
  • Team:SF Bay Area DIYBio/Description
    ...r'' bacteria - sunscreen made ''by'' bacteria. We are going to make e.coli bacteria create shinorine, a UV absorbing compound that is currently being extracted ...e wheel combined. This project makes use of Genetic Engineering to force a bacteria to create a compound that it would never make and then use the tools of evo
    7 KB (1,177 words) - 03:00, 22 November 2015
  • Team:BostonU/Notebook/Protocols/Bacterial
    ...style="color:#FF9966;">LB broth and LB agar plates</a> to grow E. coli K12 bacteria, the chassis for our cloning constructs.</li> ...9966;">grow up overnight bacterial cultures from frozen stocks</a> - these bacteria contained relevant backbones and inserts that were previously created by ou
    17 KB (2,925 words) - 00:19, 19 September 2015
  • Team:UNAM-CU/Modeling
    <h3>Bacteria</h3> that is present at the bacteria membrane is thought to detect the levels on
    18 KB (2,831 words) - 03:32, 19 September 2015
  • Team:Birkbeck/pp
    ...University of London and UCL, discuss what the average concentration of TB bacteria is in a sputum sample, and whether there is a relationship between concentr ...ine drugs, making the disease incredibly difficult to treat, is becoming a growing problem around the world. As a result of developments such as these, antibi
    14 KB (1,948 words) - 22:27, 18 September 2015
  • Team:Toronto/Description
    ...ser to visualize the effects of the addition of a synthetically engineered bacteria into a predefined microbiome. This visualization is generated through a pro <p>In concert with our web-based tool, we genetically engineered E. coli bacteria for the purpose of bioremediation. Our engineered E. coli supplements the e
    17 KB (2,581 words) - 04:31, 20 November 2015
  • Team:Cornell/Practices
    ...o help move our discoveries beyond the lab bench and meet the demands of a growing consumer market. Through collaborations with these hatcheries, which battle ...he CEA approach’s relevance to synthetic biology, in anticipation of the growing demand for synthetic biology-based solutions to global issues. They arrived
    24 KB (3,769 words) - 03:47, 19 September 2015
  • Team:Lethbridge HS/Practices
    ...closed in a matrix of sugars and extracellular DNA, this helps to hold the bacteria together like'); ...nd hubbard tanks. Because of biofilms and impossibility in eradicating the bacteria in these tubs and tanks, these are now largely obsolete in treating burn p
    40 KB (5,736 words) - 03:54, 19 September 2015
  • Team:Paris Bettencourt/Notebook/Riboswitch
    The day after I had colonies growing, nothing on my control <li>Inoculated at 4:00 pm in LB Cm 25 bacteria from a culture ( red —> RFP ) with different AdoCbl concentrations in ord
    58 KB (9,549 words) - 16:04, 16 September 2015
  • Team:Yale/methods
    ...ator with a bubbling CO2 source. We also determined which media to use for growing our strains, both for liquid and solid cultures. Once we were able to succe ...sing LB in an effort to reduce contamination problems. Our other rhizobium growing conditions were taken from literature sources; we did not have any issues w
    39 KB (5,685 words) - 02:51, 19 September 2015
  • Team:Washington/Practices
    ...wberries, identifying fluorescent proteins and chromoproteins in yeast and bacteria, and performing a pH test. You can view the worksheet from our activity he <p>Recently, iGEM has taken steps to engage with its rapidly-growing group of alumni. At the University of Washington, we are following this le
    19 KB (2,508 words) - 02:26, 19 September 2015
  • Team:Oxford/Backdrop
    ...h bacteria confer themselves protection against antimicrobial drugs is the growing of biofilms. Biofilms, or “bacterial slime”, are responsible for a whol ...heir own right but are especially problematic when they’re combined. The bacteria, already constantly evolving to afford themselves more innate resistance ag
    4 KB (528 words) - 10:04, 17 July 2015
  • Team:IIT Kharagpur/test
    ...first century - Food Insecurity.</br></br> Apart from obvious challenge of growing population, depreciating resources and inequitable distribution of wealth. It has been 4 decades since it is known that bacteria possess cell to cell communication capability using a phenomenon known as Q
    14 KB (1,915 words) - 05:23, 18 September 2015
  • Team:IONIS Paris/Practices
    ...ion popularizing science through educative mini-games around the life of a bacteria. Will we cure patient from a growing infectious disease that is spreading and devastating the world?
    48 KB (6,850 words) - 15:25, 1 October 2015
  • Team:Bordeaux/Practices
    <p align="justify" style="text-indent: 3vw;"> When it comes to bacteria however, what point of view do we have? Humans generally have a vision of t ...organism is understood, synthetic biology is able to do everything with a bacteria, a yeast or animals bigger, thinking that are like <b>"machines"</b>. But i
    48 KB (7,540 words) - 03:18, 19 September 2015
  • Team:BABS UNSW Australia/Test
    ...ained on two plasmids and integrated with the pHlow system, will ensure no bacteria which leave the lab remain invasive and prevents horizontal gene transfer. ...ichia coli K12, a small, food-grade organism Lactococcus lactis and a slow-growing cyanobacteria, Synechocystis sp. PCC6803. Each of these are exciting or use
    7 KB (993 words) - 01:28, 27 August 2015
  • Team:ETH Zurich/Modeling/Reaction-diffusion
    ....</b> Activation of the AHL module at 10 nL and 100 nL with a logistically growing population.]]</li> <li>High lactate production rate (cancer cell), doughnut of bound bacteria (<b>ON-ON</b>)</li>
    25 KB (4,290 words) - 03:42, 19 September 2015
  • Team:BABS UNSW Australia/bgcover
    ...ained on two plasmids and integrated with the pHlow system, will ensure no bacteria which leave the lab remain invasive and prevents horizontal gene transfer. ...ichia coli K12, a small, food-grade organism Lactococcus lactis and a slow-growing cyanobacteria, Synechocystis sp. PCC6803. Each of these are exciting or use
    9 KB (1,105 words) - 04:45, 5 September 2015
  • Team:UNA Honduras/notebook.html
    generate our synthetic bacteria and to update what has been done.</span></p> Laboratory practices on insertion of plasmids to transform bacteria taken by
    478 KB (57,308 words) - 00:57, 19 September 2015
  • Team:UMaryland/Results
    ...ried antibiotic resistance genes, there would be ample concern about other bacteria acquiring this resistance through horizontal gene transfer. However, if the ...despread use of antibiotics and growing population of antibiotic resistant bacteria.</p>
    17 KB (2,539 words) - 04:00, 19 September 2015
  • Team:Technion HS Israel/Experiments
    An overnight culture of the bacteria harboring the genetic circuit (based on LuxR (BBa_C0062), AiiA (BBa_C0060), <li> - Bacteria samples:
    5 KB (761 words) - 20:20, 18 September 2015
  • Team:Bordeaux/Practices2
    <p align="justify" style="text-indent: 3vw;"> When it comes to bacteria however, what point of view do we have? Humans generally have a vision of t ...organism is understood, synthetic biology is able to do everything with a bacteria, a yeast or animals bigger, thinking that are like <b>"machines"</b>. But i
    45 KB (6,978 words) - 15:08, 18 September 2015
  • Team:SYSU-Software/Vailidation
    <h3 class ="Boli">Doesn't tetracycline kill your bacteria?</h3> ...cteria in the earlier period, the resources and space made it possible for bacteria to grow more rapidly.</p>
    18 KB (2,729 words) - 03:28, 19 September 2015
  • Team:Genspace/Safety
    ...deally, be deployed in a device placed along the canal with the engineered bacteria contained by a membrane that allows small molecules to pass through but not ...to the canal would be to place the biosensor bacteria into a kit where the bacteria are in a container that canal water is added to. The kit would contain blea
    150 KB (16,094 words) - 02:04, 19 September 2015
  • Team:UNA Honduras/project/experiments-and-protocols.html
    ..."font-size: 12pt; line-height: 107%;">So we learned first how to propagate bacteria in sterile save bacteria in frozen vials.</span></p>
    217 KB (29,440 words) - 02:20, 19 September 2015
  • Team:Central Calgary/notebook
    Made fresh bacterial culture of E. coli, growing at 37°C for 3 hours. -Products secreted out of the cell BACTERIA - have DNA
    53 KB (8,632 words) - 03:57, 19 September 2015
  • Team:SYSU-Software/Validation
    ...cancer. Therefore we picked a UV sensor and a toggle switch that gives the bacteria two states of different color. If the circuit works, the expected result wo <h3 class ="Boli">Doesn't tetracycline kill your bacteria?</h3>
    19 KB (2,877 words) - 16:47, 1 October 2015
  • Team:TU Delft SamanthasPage/Lab
    ...phase. Until 2 hours, the bacteria are in the lag phase of growth. This growing behavior, in the order lag phase - exponential phase - stationary phase, is ...age"><figcaption>Fig. 2: curli cell from model where attachment of csgA to growing curli chain is completely random. </figcaption></figure>
    58 KB (7,754 words) - 11:30, 18 September 2015
  • Team:TU Delft SamanthasPage/Modeling
    ...phase. Until 2 hours, the bacteria are in the lag phase of growth. This growing behavior, in the order lag phase - exponential phase - stationary phase, is ...age"><figcaption>Fig. 2: curli cell from model where attachment of csgA to growing curli chain is completely random. </figcaption></figure>
    50 KB (7,373 words) - 23:16, 18 September 2015
  • Team:TU Delft/Modeling
    ...phase. Until 2 hours, the bacteria are in the lag phase of growth. This growing behavior, in the order lag phase - exponential phase - stationary phase, is ...gcaption><b>Fig. 11</b>: curli cell from model where attachment of csgA to growing curli chain is completely random. </figcaption></figure>
    49 KB (7,378 words) - 00:13, 21 November 2015
  • Team:MIT/Protocols
    ...res with <i>E. coli</i>. The media contains all the salts needed for both bacteria but not the carbon source (filter paper) due to the carbon source not being <li> if growing from another liquid culture, 100 uL should be plenty (replacing the 1 colon
    103 KB (17,193 words) - 00:41, 19 September 2015
  • Team:Tec-Chihuahua/Project
    ...lent functionalization of carbon nanotube materials is becoming an area of growing fundamental and industrial importance (Sobhi, <i>et. al</i>, 2007).</p> ...sformation is the introduction of foreign genetic material in prokaryotes (bacteria) and non animal eukaryotic cells such as fungi, algae or plants and transfe
    38 KB (5,139 words) - 03:30, 19 September 2015
  • Team:Vilnius-Lithuania/HumanPractices/Ethics
    ...me kind of miscommunication. When scientists talk about GMO having in mind bacteria, people think that all the information they hear is about plants or animals ...n with Ministry of Environment. We are creating a control mechanism for GM bacteria and they are taking a part in preparation of the main GMOs law, by asking o
    19 KB (3,252 words) - 16:47, 2 October 2015
  • Team:IISER Pune/Projects
    ...4 months. The reason for this large gap is that M. tuberculosis is a slow growing pathogenic bacterium with a doubling time of approximately 24 hours in tiss ...m tuberculosis is a slow-growing bacteria and hence, the detection of this bacteria in patient samples using present techniques requires a longer duration.
    11 KB (1,779 words) - 16:53, 18 November 2015
  • Team:London Biohackspace/Test wikiwiki
    ...is welcome, so get involved! It's fascinating, the field and community is growing all the time, together with the ability of amateurs to do cool stuff. Over * [[Project:Growing bacteria|Growing V. fischeri]]
    8 KB (1,287 words) - 22:15, 12 September 2015
  • Team:UCSC/Practices
    ...istribution, and geopolitical economics; Environment - climate, effects of growing a carbon source for fuel production, and effects of the current energy indu ...n up to 2% butanol, and, as an archaea, it is fundamentally different from bacteria and other natural microbes, so dangerous gene transfer is not a concern.
    11 KB (1,517 words) - 07:56, 18 September 2015
  • Team:UCSC/Bioethics
    ...istribution, and geopolitical economics; Environment - climate, effects of growing a carbon source for fuel production, and effects of the current energy indu ...n up to 2% butanol, and, as an archaea, it is fundamentally different from bacteria and other natural microbes, so dangerous gene transfer is not a concern.
    11 KB (1,499 words) - 23:13, 16 September 2015
  • Team:SVA-NYC/Notebook
    ...lastic bags of soil when they are sealed is not caused by humidity, but by BACTERIA!<br> - Darya began experimenting with growing seeds in a “Soiled” plexi cutout. She will resume documenting a few dif
    35 KB (5,397 words) - 16:18, 10 November 2015
  • Team:NJAU China/Results
    ...es of bacteria cannot grow on the medium. Therefore, if there are colonies growing on the culture medium, the conjugation process is bound to occur. The funct ...r the conjugation, co- cultivate the mixed E.coli, there are some colonies growing on the Double resistance plate.</p>
    17 KB (2,676 words) - 03:53, 19 September 2015
  • Team:NEFU China/Notebook
    ...mily:arial,helvetica,sans-serif"><span style="font-size:14px">Our team was growing. We communicated with previous iGEMers in our school to get more insights i ...still proceeded. We encountered difficulty in the electrotransformation of bacteria, which delayed our project but was eventually resolved after multiple attem
    87 KB (13,477 words) - 18:31, 18 September 2015
  • Team:Pasteur Paris/Practices
    - With bacteria, we can use a very complicated pathways to produce product that couldn't be - sensibility of biological system: Will the bacteria survive the conditions of plastic waste treatment? <br>
    16 KB (2,581 words) - 22:24, 18 September 2015
  • Team:UCL/Notebook
    ...ng electroporation and the other chemical transformation. We incubated the bacteria overnight.</p> ...pathway in mammalian cells which is central to our project as it involves bacteria interfering with humans</li>
    60 KB (7,302 words) - 00:37, 19 September 2015
  • Team:UCL/Notebooktrial
    ...ng electroporation and the other chemical transformation. We incubated the bacteria overnight.</p> cells which is central to our project as it involves bacteria interfering with humans</li>
    39 KB (5,915 words) - 03:18, 28 August 2015
  • Team:KU Leuven/Research/Methods
    ...lasmid PCP20. Afterwards, the temperature sensitive plasmid was removed by growing the cells overnight at 42°C. In a third step, the <i>tar</i> knock-out str ...he environment. When a quorum has been reached, AHL diffuses back into the bacteria and binds the transcriptional regulator CviR. This activates the expression
    45 KB (6,730 words) - 09:34, 20 October 2015
  • Team:Marburg/Safety
    ...CDI cells will not be able to survive after they were used as therapeutic bacteria, we plan to implement a killswitch.</p> ...switch flips to a “ready to kill” state. When the genetically modified bacteria then leave the gut and get in contact with oxygen again, the toxin is expre
    22 KB (3,191 words) - 15:02, 18 September 2015
  • Team:UFSCar-Brasil/notebook.html
    <p>Our proposal is building a bacteria carrying out the production of D-limonene via limonene synthase. To enable our insect repellent cream, in order to sustain the bacteria to the required metabolites building and posterior enzyme activities. In th
    4 KB (653 words) - 22:16, 16 September 2015
  • Team:Technion HS Israel/Protocols
    <li> - Bacteria samples: Prepare 5 ml starter by growing the cells in 5ml LB medium + appropriate antibiotics (5 µl of chlorampheni
    13 KB (1,995 words) - 20:35, 18 September 2015
  • Team:Oxford/Test/Chemicals
    ...<li>It is most famous for its use in the Gram method of classifying bacteria - “Gram positive” = walls are stained purple, “Gram negative” = wal ...on ampicillin plates. Therefore we could ensure that the bacteria that was growing on our plate containted the plasmid that we wanted.
    39 KB (3,921 words) - 21:39, 18 September 2015
  • Team:ANU-Canberra/background
    In the International Year of Light, it is apt to consider the growing field of optogenetics - the control of cellular dynamics using light. In it ...ial cells, thus limiting the concentrations of NAD that can be achieved in bacteria for biomanufacturing purposes. We propose that suspending <i>de novo</i> pr
    16 KB (2,156 words) - 13:46, 18 September 2015
  • Team:NRP-UEA-Norwich/Results
    <p class="space20">Both glycogen extracts from GlgB induced and non-induced bacteria weighed exactly the same. It was expected that there would not be a discern ...class="space30">We aimed to determine whether the glycogen extracted from bacteria expressing GlgB and GlgX had different structures. We wanted to see if ther
    94 KB (13,354 words) - 10:22, 21 October 2015
  • Team:Slovenia HS/Conclusions
    them at lower temperatures. We tried growing them at 20 °C and 16 °C, at which point, quite and BdhB) would be ligated together so that the bacteria are able to perform the conversion
    9 KB (1,419 words) - 22:58, 18 September 2015
  • Team:Slovenia HS/TeamSlovenia HS Lab Safety Recipes.html
    Bacteria were inoculated on the new LB plate in laminar:<br>
    23 KB (3,686 words) - 11:24, 18 September 2015
  • Team:Oxford/Chemicals
    ...<li>It is most famous for its use in the Gram method of classifying bacteria - “Gram positive” = walls are stained purple, “Gram negative” = wal ...on ampicillin plates. Therefore we could ensure that the bacteria that was growing on our plate containted the plasmid that we wanted.
    39 KB (3,972 words) - 00:58, 19 September 2015
  • Team:TU Dresden/Human Practices/Long/Translation Poster
    ...small portion of bacteria is required, one portion of the media rich with bacteria is discarded and the other portion goes to the lagoon, where they meet the
    3 KB (530 words) - 21:29, 11 September 2015
  • Team:Goettingen/Notebook
    - Will the bacteria survive and work continuously or die after their application growing bacteria is tricky; two of 4 cultures may be growing but are very slow. They were plated on solid medium in order to see if they
    47 KB (7,545 words) - 11:19, 18 September 2015
  • Team:Washington/Paper Device
    <p>Paper diagnostics is a fast-growing area of bioengineering. It is especially appealing for detecting pathogens ...oup at the University of Alberta, which has performed some work in growing bacteria and mammalian cells on paper [1,2]. We especially drew from a 20XX article
    18 KB (2,221 words) - 03:31, 19 September 2015
  • Team:Cork Ireland/Practices
    ...e regarding synthetic biology was diverse. Some saying that it is “using bacteria to make vaccines and drugs” hoping to use bacteria to detect viruses and the things that make people sick. They all seemed ver
    44 KB (6,534 words) - 03:59, 19 September 2015
  • Team:Paris Bettencourt/Sustainability/Continuity
    ...allow the manufacturer to detect contamination, and check that what he is growing is exactly what he wants to grow.</li> ...ble to make a strain that fullfills its nutrient-producing functions while growing as fast as the wild type, so we found a workaround: the cells that people u
    25 KB (4,059 words) - 20:43, 18 September 2015
  • Team:TU Dresden/Human Practices/Social Outreach/Translation Poster
    ...small portion of bacteria is required, one portion of the media rich with bacteria is discarded and the other portion goes to the lagoon, where they meet the
    3 KB (531 words) - 22:27, 11 September 2015
  • Team:Westminster/Biocontainment
    ...ct is to ensure that the organism cannot escape the media from which it is growing as the media itself also contains molecules vital to the operation of the r ...ds to survive. The likelihood of both plasmids being transferred to native bacteria is unlikely (Hayes, 2003). This may still encounter issues regarding horizo
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  • Team:Cork Ireland/Project
    (viruses and bacteria) and the diagnosis of genetic conditions (inherited mutations, copy number From our policy and practice work during the summer we learned of the growing need for a detector to quickly analyse whether or not a patient was infecte
    55 KB (7,857 words) - 15:10, 9 November 2015
  • Team:UB Indonesia/Notebook
    ...is excreted by the bacteria, and inactivates the antibiotic outside of the bacteria</p> <div class="desc"><p> 1. Growing competent Escherichia coli strain BL21 and DH5α</p>
    24 KB (2,665 words) - 03:10, 19 September 2015
  • Team:Oxford/Team
    ...As chief chemist I have been in charge of making beads to encapsulate the bacteria (and vodka) as well as having far too much fun making up litres and litres When not growing on trees, I am usually somewhere in the lab running microscopy and plate re
    18 KB (2,148 words) - 11:22, 20 November 2015
  • Team:UNIK Copenhagen/Green Lab
    ...a photosynthetic organism and thus produce oxygen, where yeast and (most) bacteria are heterotrophic organism that cannot produce oxygen. Being able to do pho ...oss grow or watching paint dry, opt for the latter. Moss grows slower than bacteria and yeast, but faster than most higher plants. Moss is relatively simply to
    5 KB (863 words) - 20:01, 17 September 2015
  • Team:Kent/Results
    ...teria to be grown in heme-containing medium. We postulate that growing our bacteria in a medium containing heme before the imaging would allow cytochrome foldi ...to use our nanowires to conduct electricity which would be produced by the bacteria themselves, by transferring electrons from the bacterial electron transport
    17 KB (2,786 words) - 17:43, 1 October 2015
  • Team:Uppsala/Safety
    ...don’t take these safety precautions. Therefore all waste that contained bacteria was treated with jodopax, an iodine based disinfectant, before being discar ...s when working with it; it was handled and kept in a fume hood. Since this bacteria consumed naphthalene as a carbon source it was always handled with gloves a
    10 KB (1,786 words) - 13:24, 14 November 2015
  • Team:Penn/Notebook
    ...sources (1) or (3), use a clean, sterile pipet tip to scrape a tiny bit of bacteria (even the very smallest amount is okay) and then pipet up and down into the ...ain a sterile SNAP top culture tube and label it with the strain ID of the bacteria you&rsquo;d like to grow</li>
    23 KB (3,584 words) - 03:10, 17 September 2015
  • Team:Marburg/Labbook/CDI
    ...proven. LB-Plates (CAM, 0.8mM IPTG) with 0,27% Agar were used to give the bacteria the possibility to swarm across the plate. <br> The result is that the bacteria stopped to grow a short distance before getting in contact with each other
    107 KB (15,981 words) - 14:33, 16 September 2015
  • Team:UNIK Copenhagen/Moss
    ...ing moss for our experiments rather than the usual chassis organisms, like bacteria or yeast? There are three main reasons for this: ...a photosynthetic organism and thus produce oxygen, where yeast and (most) bacteria are heterotrophic organism do not. Being able to do photosynthesis and prod
    2 KB (369 words) - 14:50, 6 September 2015
  • Team:Freiburg/Glossary
    ...e the lacI system in cells; often used to induce protein overexpression in bacteria </td></tr> ...ia-Bertani-medium, a nutritionally rich medium, primarily used for growing bacteria </td></tr>
    14 KB (1,886 words) - 02:13, 19 September 2015
  • Team:Purdue/TheProblem
    ...ent (Sims). Third generation production of biofuels involves using quickly growing cellulosic biomass such as algae. Fourth generation involves plants specifi <p>Biodigestion involves the decomposition of organic matter by bacteria in an anaerobic environment in order to produce a fuel source (biogas, bioe
    16 KB (2,287 words) - 01:28, 20 November 2015
  • Team:Aalto-Helsinki/Design
    ...s for propane detection. Basically, terrific broth (TB) containing induced bacteria would be added into 22 ml GC-vials. The cells are incubated different lengh ...ed four different antibiotics which were needed to keep plasmids inside of bacteria. Details about standards, peaks etc. for GC/MS can be seen in <a href="http
    10 KB (1,529 words) - 15:45, 18 September 2015
  • Team:ZJU-China/Project/Protocol
    2. Growing overnight @37℃ ...into 2 ml centrifuge tube and centrifuge at 3000 rpm for 15 min to collect bacteria.
    37 KB (4,772 words) - 14:38, 17 September 2015
  • Team:Slovenia HS/Background
    ...acroelements ( C,H,N,O,P and S) and microelements (Fe,Se,Ca, Na…) in its growing environment.</p> ...ent in the bacterium Clostridium acetobutylicum, into E. coli. Thus E.coli bacteria must be modified by synthetic biology and genetic engineering methods. We
    11 KB (1,667 words) - 22:57, 18 September 2015
  • Team:BIT/Interlab Study
    The cultivation of our bacteria was performed in tubes filled with 7 mL LB liquid medium. The cultures were ...g and increased after 8 hour. We speculated maybe the bacteria had stopped growing but the GFP was still increasing slowly. According to the data of BBa_K8230
    16 KB (2,006 words) - 02:11, 19 September 2015
  • Team:Stanford-Brown/Plastic
    ...o send a sample of bacteria into a space station or colony and having that bacteria manufacture plastic is an exciting prospect for our team.</p> <p><b>Figure 14</b> Growing biopigment producing <i>E. coli</i> to use to print on plastic</p>
    11 KB (1,639 words) - 03:59, 19 September 2015
  • Team:Austin UTexas/Collaborations
    ...lly, they helped test our newly created plasmids for the caffeine study by growing the different strains we had engineered. We also helped them in creating bi
    2 KB (352 words) - 03:10, 19 September 2015
  • Team:MIT/Measurement
    ...grown separately?). This would inform us on how we should engineer the two bacteria to communicate to achieve the desired population dynamics. Distinguishing t ...oncerns about metabolic load. Hemacytometry could not be used because both bacteria move around too much to reliably count. Flow cytometry can be used to deter
    5 KB (805 words) - 03:50, 19 September 2015
  • Troubleshooting/Transformation
    ...blem. Below are some common problems that can result in a lawn of bacteria growing instead of single, isolated colonies after transforming your DNA. ...fficiency and you transform plasmid, you can sometimes get a lawn of cells growing. This most often occurs if you have a high plasmid concentration going into
    12 KB (1,892 words) - 18:16, 16 July 2015
  • Team:IISER Pune/Description
    ...es obtained from patients. The standard stain test does not work for these bacteria as they have a complex mycolic acid layer which prevents uptake of the dyes ...losis</i> will be performed in an attempt to accelerate the growth of slow growing <i>Mycobacteria</i>. Mathematical modeling and computer simulations will be
    3 KB (564 words) - 18:53, 13 August 2015
  • Team:Freiburg/Project/Methods/ProtPur
    <h2 class="sectionedit2">Using the natural expression machinery: Growing cells</h2> The genetic information for our antigens was transformed into the bacteria we used (see: <a class="urlextern" href="https://2015.igem.org/Team:Freibur
    7 KB (1,059 words) - 10:29, 29 August 2015
  • Team:Freiburg/Project/Protein Purification
    <h2 class="sectionedit2">Using the Natural Expression Machinery: Growing Cells</h2> The genetic information for our antigens was transformed into the bacteria we used (see: <a class="urlextern" href="https://2015.igem.org/Team:Freibur
    11 KB (1,548 words) - 21:53, 18 September 2015
  • Team:Dundee/Forensic Toolkit/FluID
    ...of polyamine synthesis, degradation and transport is tightly controlled in bacteria. In <i>E. coli</i>, two of three identified transport systems are ABC trans ...s that on comparison with the uninduced control, the induced cultures stop growing when LbpA expression is induced. After ~7 hours, it is possible that the em
    41 KB (6,123 words) - 12:47, 4 October 2015
  • Team:Macquarie Australia/Practices/Implementation/SrategicPlan
    ...capability to the market. Successful engineering of hydrogen gas-producing bacteria within a contained system would have the dual benefits of modular energy pr ...ven conservative assumptions projecting renewable energy to be the fastest growing sector at 6.7% annually (Sadorsky, 2012). While the world is still heavily
    21 KB (3,027 words) - 08:39, 5 November 2015
  • Team:DTU-Denmark/Project/Background
    ...diverse class of short, bioactive peptides isolated from plant, fungi, and bacteria.&nbsp;Clinical uses of NRPs include antibiotics, immunosuppressants, antifu ...like an assembly line (Figure 2) [9]. At the end of the assembly line, the growing peptide is released as a linear or cyclic peptide.&nbsp;</p>
    68 KB (8,952 words) - 15:15, 9 November 2015
  • Team:UCLA/Project
    ...ng after, however, the idea behind silk processing still remains the same: growing silkworms, from which silk is extracted, cleaned and further processed. ...is a few steps further: instead of growing silkworms in factories, we want bacteria to be our microbial factories, producing silk proteins as it would any othe
    11 KB (1,692 words) - 00:03, 2 October 2015
  • Team:Amsterdam/Team
    ...the results into actionable steps in the lab. When not growing pathogenic bacteria in her garage, Tamar can be found climbing in one of Holland’s many indoo ...first time he, the IT guy, was teaching us, the biologists, how to culture bacteria and work aseptically. In exchange we helped him out in building software to
    11 KB (1,418 words) - 17:11, 1 October 2015
  • Team:MIT/Team
    ...gy. Nick hopes to develop new methods of metabolic wiring between multiple bacteria to create a highly integrated consortium with unique intercellular properti ...earnt about the possibilities for "hacking" real living organisms, such as growing human chins on the backs of mice or maybe even mouse chins on the backs of
    21 KB (3,094 words) - 20:04, 13 September 2015
  • Team:Bielefeld-CeBiTec/Protocols
    ...sion Solution. Transfer the cell suspension to a microcentrifuge tube. The bacteria should be resuspended completely by vortexing or pipetting up and down unti <li>Add 1 mL bacteria suspension in every well of the 48-wellplate.</li>
    85 KB (11,967 words) - 09:07, 13 October 2015
  • Team:Harvard BioDesign/Platform
    ...prepared according to the slide preparation protocol and to visualize the bacteria and yeast, we stained the slides according to the Gram Stain protocol. We first tested our assay on our control strains, growing both the overproducer and null strain overnight in LB liquid culture, then
    22 KB (3,016 words) - 21:27, 21 November 2015
  • Team:Warwick/Project5
    - <br>Inoculated culture with RFP bacteria. - <br>Only 2 colonies on the 4 plates of bacteria grew (background growth is 0 via
    20 KB (3,025 words) - 20:11, 18 September 2015
  • Team:Westminster/Practices
    ...ing to investigate the potential of our engineered <i>Escherichia coli</i> growing in wastewater. <br><br> ...hrough to the lower level which is populated with nitrifying bacteria. The bacteria aid the removal of ammonia through oxidation of ammonia to nitrite to nitra
    20 KB (3,318 words) - 00:57, 19 September 2015
  • Team:BGU Israel/Practices
    ...We ended the workshop with an experiment on growing bacteria, using the bacteria they naturally have on their hands.<br /><br />
    33 KB (4,347 words) - 16:30, 18 September 2015
  • Team:Hamburg/Notebook
    <li>please restart growing step in the 37°C room.</li> <li>other samples are not usable either (too much bacteria)
    51 KB (7,848 words) - 19:09, 18 September 2015
  • Team:Aalto-Helsinki/Outreach
    ...roscopes to check out gram-stained bacteria with, amazing glow-in-the-dark bacteria and even a gene gun! Children could also colour their own superhero bacteri ...in both the University of Helsinki and Aalto University to ensure an ever growing number of applicants for the Aalto-Helsinki iGEM team.</p>
    17 KB (2,634 words) - 13:32, 18 September 2015
  • Team:UCLA/Team/Meet the Team
    <font color="#a88d2e">[Why iGEM?]</font> I am truly fascinated by our ever-growing ability to harness the power of biology and shape it for our own uses and d ...the very beginning to the very end. We get to tinker with nature and coax bacteria to work in ways never thought of before. Now add in a veritable LEGO box of
    15 KB (2,307 words) - 07:40, 18 September 2015
  • Team:IIT Madras/Practices
    ...0 children in India died in one year due to infection with super resistant bacteria passed on through mother. <p> ...c use could be called a tragedy of the commons, and it was inevitable that bacteria would develope resistance sooner or later with such widespread use of antib
    10 KB (1,537 words) - 00:50, 19 September 2015
  • Team:NTU-LIHPAO-Taiwan/Modeling
    Noticing the problem of obesity growing in Taiwan, our team NTU-LIHPAO-Taiwan make efforts to improve a designed pr ...thalamus for controlling satiety feeling. Moreover, the suicide device and bacteria survival were also considered in the simulation for safety concern. Combini
    22 KB (2,463 words) - 13:15, 18 September 2015
  • Team:BGU Israel/test/Practices
    ...<b>We end the workshop with experiment.</b> Growing bacteria, using the bacteria they naturally have on their hands.<br /><br />
    29 KB (3,810 words) - 14:52, 6 September 2015
  • Team:Yale/team
    ...ase and ligation-free cloning (ELIC) and playing &quot;Toxic&quot; for her bacteria. After college, she hopes to go to medical school and find a career that co ...ysician scientist, studying human gut microbiota and engineering patients' bacteria to treat their bodies. He is a semi-professional juggler and is often seen
    34 KB (3,856 words) - 03:43, 19 September 2015
  • Team:HokkaidoU Japan/Modeling
    First, we want to describe the number of host cells growing without toxicity of the peptide as the differential equation. The logistic <p>We can expect that the amount of antimicrobial peptides and population of bacteria will be constant at last regardless of parameter &alpha; and &beta; value.
    5 KB (751 words) - 02:44, 19 September 2015
  • Team:UFSCar-Brasil/part3.html
    <p>Similarly to what occurs with the smtA, the zur curve is growing rapidly in the early hours and reaches a plateau after approximately four h ...tA protein. This information is important to determine the lifetime of our bacteria (after starting, how long it will take to killswitch activate?) and if that
    23 KB (3,338 words) - 21:32, 18 September 2015
  • Team:Paris Saclay/Practices
    .../Paris_Saclay-HP-Collaboration.jpg" class="photoprofil" /><p>The incessant growing number of teams joining iGEM competition increases possibilities of collabo ...fer with their Bware project. We chose to control the life or death of our bacteria using temperature, allowing them to survive in a defined range of temperatu
    20 KB (3,114 words) - 00:04, 19 September 2015
  • Team:Northeastern Boston/Attributions
    ...ew Hampshire with a BS, and my undergraduate research involved methods for growing microalgae for biofuel production and genetic engineering of the microalgae ...herichia coli. Due to its relative simplicity, studying basic processes in bacteria will render mechanistic insights that would be difficult to attain directly
    13 KB (1,938 words) - 03:50, 19 September 2015
  • Team:BABS UNSW Australia/Notebook2
    ...pseudoknot G-blocks - all with Daniel's help. We also updated our rapidly growing DNA parts/biobricks database. ...in as a 4th antibiotic. It seems very successful in its job of killing all bacteria (not so good for us though).
    26 KB (4,201 words) - 01:40, 8 October 2015
  • Team:Columbia NYC/Notebook
    <p>· Bacteria involved, methods, what's natural, etc. Just WOW factors </p> ...tubes were obtained each tube had 5 mL of LB and then when you insert the bacteria it is MegaX and Nissle (it was just poked not an exact amount was taken out
    47 KB (8,456 words) - 00:54, 19 September 2015
  • TeamSlovenia HS Practices.html
    using bacteria yet in Slovenia and our project is unique in that respect, so we have decid and food, but on the other hand, many other countries such as the USA are growing more
    21 KB (3,273 words) - 14:17, 18 September 2015
  • Team:UB Indonesia/Protocols
    ...is excreted by the bacteria, and inactivates the antibiotic outside of the bacteria </p><p>After growing up a part from a distribution or request, you'll want to miniprep it. At iG
    22 KB (3,287 words) - 03:12, 19 September 2015
  • Team:Aachen/Lab/Bioreactor/Hardware
    How can we find out if there is really bacteria growing in our reactor? The common way is doing OD measurements. But if there is on To keep the whole measurement sterile, the medium with the bacteria flows through silicon tubes and a transparent glass structure through the d
    7 KB (1,163 words) - 15:58, 5 October 2015
  • Tracks/Art Design
    ...ioengineering from Harvard, where she worked on producing hydrogen fuel in bacteria and making photosynthetic animals. </p> ...h Fellow at Central Saint Martins College of Art and Design, she pioneered growing clothing using living organisms and authored Fashioning the Future: Tomorro
    20 KB (3,092 words) - 19:55, 24 April 2015
  • Team:UChile-OpenBio/Team
    ...y.</p><img alt="Severine" src="https://static.igem.org/mediawiki/2015/d/d2/Bacteria-presentadora.png" /></div> ...that's why it is a pleasure to work with him. Sometimes he gets angry with bacteria when he design the reactor but is ok.
    19 KB (2,584 words) - 21:01, 18 September 2015
  • Team:IONIS Paris/Attributions
    ...ommercializes innovative fluid handling solutions for a variety of rapidly growing applications where fluid control is critical.</p> <p>Our reality game using bacteria<br></p>
    22 KB (3,041 words) - 12:57, 14 October 2015
  • Team:NAIT Edmonton/Practices
    ...e are approximately sixty million peer-reviewed articles and the number is growing exponentially every year. That’s an avalanche of information that can be ...ortisol is known to cause immune system failure. (That’s not the kind of bacteria we want!)</li>
    34 KB (5,355 words) - 02:54, 19 September 2015
  • Team:Bordeaux/AchievementsNotebook
    ...15/6/60/Growing_Medium_of_E.coli_VC.pdf"target="_blank"><font size="3" ><B>Growing Media of <I>E.coli</I></B></font></a> </li> ...igem.org/Team:Bordeaux/Bacteria_results" style=" color: #FF5E00;"> &#9755; Bacteria Results </h6>
    4 KB (578 words) - 02:36, 19 September 2015
  • Team:TAS Taipei/team
    ...rns out to be E. coli- not disappointed) I also joined iGEM because of the growing role synthetic biology plays in health, food, and environmental issues. Rat ...un in the sense that I could learn to manipulate genes to allow us to make bacteria do things that they were not originally able to do. So, I decided to give i
    24 KB (3,563 words) - 11:08, 30 October 2015
  • Team:Aachen/Lab/Libraries
    ...ations. Furthermore, IPTG-induced gene expression makes sense when growing bacteria on a difficult substrate such as methanol.
    6 KB (890 words) - 22:00, 18 September 2015
  • Team:Birkbeck/Description
    <p>Personal medicine and non-invasive biological measurements are the fastest growing areas in biotechnology. Viruses and bacteriophages are central to a number ...yielded great results regarding the proof of concept for quickly detecting bacteria using phages, and we would like to take the technology one step further by
    4 KB (630 words) - 21:56, 18 September 2015
  • Team:Cornell/policy
    ...to help take our discoveries from the lab bench and meet the demands of a growing consumer market. Through conversations with these hatcheries, which deal wi ...py to share our experiences working with Flavobacterium psychrophilum, our bacteria of interest that we were trying to tackle with fishPHARM. Because F. psychr
    8 KB (1,153 words) - 00:26, 14 September 2015
  • Team:Cornell/futurework
    ...n B peptides utilized in our project aren’t effective against a specific bacteria, the same system utilizing a different agent could be utilized. Antimicrob ...the agriculture and human health. With increased antibiotic resistance a growing problem, solutions such as ours will have a more prominent role in human he
    7 KB (940 words) - 03:40, 19 September 2015
  • Team:NCTU Formosa/Description
    <p>Moreover, scFv can be easily produced and displayed by bacteria as it is only 20 percent of the size of most normal antibodies<sup>[1]</sup ...t particular person. This indicates that detection for targeted drugs is a growing field and will become a prevalent pre-diagnosis for cancer treatment in the
    14 KB (2,032 words) - 15:39, 31 October 2015
  • Team:SJTU-BioX-Shanghai/Description
    ...er with salt-tolerance as well as other noble characteristics such as fast-growing, photoautotrophic, amenable to genetic engineering,capable of utilizing env .... (2014). Biodesalination: a case study for applications of photosynthetic bacteria in water treatment. Plant physiology, 164(4), 1661-1676.</i>
    6 KB (790 words) - 03:31, 19 September 2015
  • Team:Nankai
    ...ustrial production. Most importantly, will our operation be harmful to the bacteria so that they can’t even survive? These are all we should take care of. </ ...ianjin, Zhejiang Provicnce and so on. Through communications, our team are growing and our ideas are spreading! <a href="https://2015.igem.org/Team:Nankai/Pra
    12 KB (1,667 words) - 15:58, 18 September 2015
  • Team:Sydney Australia/Description
    ...matis. Mycobacteirum is a difficult bacteria to work with as they are slow growing and difficult to work with. Consequently, the team is attempting to achieve
    9 KB (1,357 words) - 04:10, 18 September 2015
  • Team:EPF Lausanne/Notebook/Protocols
    ...r sequencing). In these cases it's possible to proceed as follows: culture bacteria in 5 x 7mL tubes of LB medium and perform the miniprep for each tube separa ...<li>Start a pre-culture of 50mL YPD or SD + Glucose with one colony. Growing at 30°C with shaking (250 rpm) for 16h to 18h untill stationary phase, OD6
    54 KB (5,322 words) - 23:45, 16 September 2015
  • Team:ETH Zurich/Interlab study
    <p>The overnight bacteria culture was diluted in Lysogenic Broth (LB) to OD600 of 0.2&plusmn;0.04 in <p>The incubator used for growing the cells was the KuhnerShakerX, model ClimoShaker ISF1-X. The throw was or
    9 KB (1,441 words) - 03:20, 19 September 2015
  • Team:IIT Delhi/theme project.html
    ...o H2S (Growth Yields and Growth Rates of. Desulfovibrio vulgaris (Marburg) growing on Hydrogen plus Sulfate and Hydrogen plus Thiosulfate as the Sole Energy S <li> &#8226; To test the working of the engineered bacteria for the reduction of the NOx and SOx gases we decided to design a prototype
    13 KB (1,562 words) - 11:58, 25 July 2015
  • Team:NU Kazakhstan/Notebook
    Figure 1: There were two types of colonies growing on bacitracin selective plate. They are expected to be S. mutans and S.sobr 1. Vector pMSP3535 for Nisin controlled expression for Gram-Positive bacteria kindly provided by Dr.Gary Dunny's Lab. <br>
    18 KB (2,876 words) - 00:23, 19 September 2015
  • Team:British Columbia/TeamAttributions
    <h4>Growing Bacteria</h4>
    371 B (57 words) - 21:14, 23 July 2015
  • Team:Tuebingen/Results
    <p>A liquid culture was inoculated with BL21(DE) bacteria containing the pETue-Dronpa construct. The culture was grown to an OD<sub>6 ...2% are on the same level as without galactose. Possibly, the cells are not growing efficiently on galactose, or there is a contamination in the galactose that
    36 KB (5,425 words) - 16:36, 9 November 2015
  • Team:Missouri Rolla/Attributions
    ...n and antimicrobial activity of the essential oil of Ocimum gratissimum L. growing in Eastern Kenya. <i>African Journal of Biotechnology</i>, 6(6).</li><li>Pr
    16 KB (1,932 words) - 20:31, 18 September 2015
  • Team:Sydney Australia/Modeling/visualisation
    ...ch we believe more accurately describes the tRNA concentration dynamics in bacteria. The resultant tool is able to visualise how the interplay of factors inclu ...itative estimation of the global translational activity in logarithmically growing yeast cells”, BMC Sys Biol, Vol 2.
    21 KB (3,221 words) - 10:56, 16 September 2015
  • Team:Paris Saclay/Notebook/August/4
    ...le bit of colony. We can suppose that the initial culture wasn't enough in growing phase. ...er time, with the same protocol than the 03/08/2015 but a fresh culture of bacteria.
    4 KB (570 words) - 21:30, 18 September 2015
  • Team:Technion Israel/Team/About Us
    ...used with anyone else's- she showed expression blatantly by growing purple bacteria using the BBa_K1357008 BioBrick from the 2015 DNA Distribution Kit. Additio
    18 KB (2,549 words) - 00:33, 19 September 2015
  • Team:Sydney Australia/TransOpt
    ...ective expression of the desired genes in convenient and fast-growing host bacteria. <sup>32 </sup> Keiler K et al. 2015, “Mechanisms of ribosome rescue in bacteria”, Nature Reviews Microbiology, Vol 13.
    55 KB (8,206 words) - 13:07, 18 September 2015
  • Team:Brasil-USP/Parts/KI
    ...All colonies are in their respective log phase, except for LB which has no bacteria indeed.
    10 KB (1,499 words) - 21:55, 18 September 2015
  • Team:WHU-China/Team
    ...ught me totally different things, and I enjoy every detail of that. I like growing up with friends, good luck to every iGEMers! ...ege of Life Science and professor of microbiology. His research focuses on bacteria natural genetic transformation mechanism.
    32 KB (4,027 words) - 03:09, 7 October 2015

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