Difference between revisions of "Team:BABS UNSW Australia/Parts"

 
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         <li>Parts
 
         <li>Parts
 
         </li>
 
         </li>
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      <li>
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                <a href="#lacto">Lactococcus</a>
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            </li>
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      <li>
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                <a href="#pknot">Pseudoknots</a>
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            </li>
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      <li>
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                <a href="#iptg">IPTG promoter + Pseudoknots</a>
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            </li>
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      <li>
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                <a href="#ph">pH promoters</a>
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            </li>
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      <li>
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                <a href="#table">Parts Table</a>
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            </li>
 
         </ul>
 
         </ul>
 
     </nav>   
 
     </nav>   
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<div id="container">
 
<div id="container">
  
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<h2> Part Documentation</h2>
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<div id = "lacto" class = "anchor"></div>
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<h4>Lactoccocus Parts </h4>
 
<h4>Lactoccocus Parts </h4>
 
<ul>
 
<ul>
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<img src="https://static.igem.org/mediawiki/2015/9/99/BABS_UNSW_Australia_LactoGblockDeusExMachina.png" style="max-width:30%;">
 
  
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<il>BBa_K1677103: Invasin with LysM signal peptide (Lactococcus)</il>
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<il><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1677103">BBa_K1677103: Invasin with LysM signal peptide (Lactococcus)</a></il>
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<ul><li>This protein-coding part encodes invasin tailored for use in <i>Lactococcus</i> species.The signal peptide region of <i>Listeria monocytogenes</i> was exchanged for a signal peptide <i>Lactobacillus plantarum</i>. The aim of this is localise the invasin protein to the outer membrane when used in <i>Lactococcus</i> species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in <i>L. lactis</i>. This part was assembled through Gibson assembly of three g-blocks designed with 20-30bp overlapping regions.</li></ul>
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<ul><li>This protein-coding part encodes invasin tailored for use in <i>Lactococcus</i> species.The signal peptide region of <i>Listeria monocytogenes</i> was exchanged for a signal peptide from <i>Lactobacillus plantarum</i>. The aim of this is localise the invasin protein to the outer membrane when used in <i>Lactococcus</i> species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in <i>L. lactis</i></ul>
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<il>BBa_K1677101: Listeriolysin (Lactococcus)</il>
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<ul><li>This part is listeriolysin codon-optimised for expression in <i>Lactococcus</i>. By co-expressing with the above LysM-INV, <i>Lactococcus</i> species can enter into mammalian cells. This part was assembled through overlap PCR of two g-blocks with a 20bp overlap. </li>
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</ul>
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</p>
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<il><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1677101">BBa_K1677101: Listeriolysin (Lactococcus)</a></il>
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<ul><li>This part is listeriolysin codon-optimised for expression in <i>Lactococcus</i>. By co-expressing with the above LysM-INV, <i>Lactococcus</i> species can enter into mammalian cells.
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</ul></ul>
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<div id ="pknot" class="anchor"></div>
 
<h4>Pseudoknots</h4>
 
<h4>Pseudoknots</h4>
 
<ul>
 
<ul>
  
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<il>BBa_K1677391: IBV Pseudoknot</il>
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<a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1677391">BBa_K1677391: IBV Pseudoknot</a>
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<ul><li>Sequence adapted from a pseudoknot sourced from the Infection Bronchitis Virus.</ul></li>
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<ul><li>Sequence adapted from a pseudoknot sourced from the Infection Bronchitis Virus.</ul>
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<il>BBa_K1677369: MMTV Pseudoknot</il>
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<il><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1677369">BBa_K1677369: MMTV Pseudoknot</a></il>
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<ul><li>Sequence adapted from a pseudoknot sourced from the Mouse Mammary Tumor Virus.</ul></li>
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<ul><li>Sequence adapted from a pseudoknot sourced from the Mouse Mammary Tumor Virus.</ul>
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<il>BBa_K1677743: TGV Pseudoknot</il>
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<a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1677743">BBa_K1677743: TGV Pseudoknot</a>
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<ul><li>Sequence adapted from a pseudoknot sourced from the Transmissible Gastroenteritis Virus.</ul></li>
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<ul><li>Sequence adapted from a pseudoknot sourced from the Transmissible Gastroenteritis Virus.</ul>
 
<ul> The above pseudoknots were submitted individually, for future use as translational regulatory mechanisms.</ul>
 
<ul> The above pseudoknots were submitted individually, for future use as translational regulatory mechanisms.</ul>
 
</ul>
 
</ul>
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<div id ="iptg" class="anchor"></div>
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<h4>IPTG-inducible promoter + Pseudoknots
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<h4>IPTG-inducible promoter + Pseudoknots</h4>
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<ul>
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<p><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1677800">BBa_K1677800: IPTG inducible promoter with IBV pseudoknot</a></p>
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<p><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1677801">BBa_K1677801: IPTG inducible promoter with TGV pseudoknot</a></p>
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<p><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1677802">BBa_K1677802: IPTG inducible promoter with MMTV pseudoknot</a></p>
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<ul><li>The above three parts are composite biobricks composed of a promoter inducible by IPTG (BBa_J04500) followed by one pseudoknot. This device allows expression of a gene with a translational delay post-induction.
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</ul></ul>
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<div id ="ph" class="anchor"></div>
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<h4>pH promoters</h4>
 
<ul>
 
<ul>
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<il>BBa_K1677800: IPTG inducible promoter with IBV pseudoknot</il>
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<a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1677300">BBa_K1677300: Lactococcus lactis p170 promoter</a>
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<il>BBa_K1677801: IPTG inducible promoter with TGV pseudoknot</il>
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<ul><li>This promoter has enhanced activity under low pH.</ul>  
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<il>BBa_K1677802: IPTG inducible promoter with MMTV pseudoknot</il>
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<ul><li>The above three parts
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</il></ul>
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<div id ="table" class="anchor"></div>
 
<h4>Parts Table </h4>
 
<h4>Parts Table </h4>
 
</html>
 
</html>

Latest revision as of 18:26, 18 September 2015

Lactoccocus Parts

    BBa_K1677103: Invasin with LysM signal peptide (Lactococcus)
    • This protein-coding part encodes invasin tailored for use in Lactococcus species.The signal peptide region of Listeria monocytogenes was exchanged for a signal peptide from Lactobacillus plantarum. The aim of this is localise the invasin protein to the outer membrane when used in Lactococcus species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in L. lactis
    BBa_K1677101: Listeriolysin (Lactococcus)
    • This part is listeriolysin codon-optimised for expression in Lactococcus. By co-expressing with the above LysM-INV, Lactococcus species can enter into mammalian cells.

Pseudoknots

    BBa_K1677391: IBV Pseudoknot
    • Sequence adapted from a pseudoknot sourced from the Infection Bronchitis Virus.
    BBa_K1677369: MMTV Pseudoknot
    • Sequence adapted from a pseudoknot sourced from the Mouse Mammary Tumor Virus.
    BBa_K1677743: TGV Pseudoknot
    • Sequence adapted from a pseudoknot sourced from the Transmissible Gastroenteritis Virus.
      The above pseudoknots were submitted individually, for future use as translational regulatory mechanisms.

IPTG-inducible promoter + Pseudoknots

pH promoters