Part Documentation
Lactoccocus Parts
BBa_K1677103: Invasin with LysM signal peptide (Lactococcus)
- This protein-coding part encodes invasin tailored for use in Lactococcus species.The signal peptide region of Listeria monocytogenes was exchanged for a signal peptide Lactobacillus plantarum. The aim of this is localise the invasin protein to the outer membrane when used in Lactococcus species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in L. lactis. This part was assembled through Gibson assembly of three g-blocks designed with 20-30bp overlapping regions.
BBa_K1677101: Listeriolysin (Lactococcus)
- This part is listeriolysin codon-optimised for expression in Lactococcus. By co-expressing with the above LysM-INV, Lactococcus species can enter into mammalian cells. This part was assembled through overlap PCR of two g-blocks with a 20bp overlap.
Pseudoknots
BBa_K1677391: IBV Pseudoknot
BBa_K1677369: MMTV Pseudoknot
BBa_K1677743: TGV Pseudoknot
The above pseudoknots were submitted individually, for future use as translational regulatory mechanisms.
BBa_K1677800: IPTG inducible promoter with IBV pseudoknot
BBa_K1677801: IPTG inducible promoter with TGV pseudoknot
BBa_K1677802: IPTG inducible promoter with MMTV pseudoknot
Parts Table
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