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Revision as of 21:39, 31 August 2015
To amplify an insert for cloning, using high-fidelity phusion polymerase.
Colony PCR is commonly used to screen for the presence of a insert in a plasmid during cloning.
Before primers can be used for PCR they need to be resuspended and diluted.
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hirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae.Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae.
UBC iGEM 2015