Team:British Columbia/Part Collection
Part Collection
Our collection of parts contains genes required for modification of imidacloprid and degradation of 6-chloronicotinic acid (6-CNA). Final parts were assembled in pSB1C3 backbone harboring the chloramphenicol resistance gene and designed to have a LacI repressor, ribosome binding site, pTAC promoter, our degradation genes of interest, and double terminator.
The three composite parts each containing a cytochrome P450 (CYP) were designed to include an N-terminal pelB signal sequence to target expression to the periplasm and a cytochrome P450 reductase (CPR) for functionality of the CYP. The CPR was also made with it’s own designated rbs, promoter, pelB signal sequence, and terminator.
Composite parts with multiple degradation genes were designed so that each gene in the construct had a dedicated rbs, promoter, and double terminator.
Parts Collection!
This interface is setup to be easily configured by changing the values of only a few variables. This layout is also built to be responsive.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed posuere eget nulla a pharetra. Suspendisse ac venenatis odio. Sed blandit posuere erat a posuere. Suspendisse odio erat, elementum sodales ante vel, malesuada rutrum turpis.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed posuere eget nulla a pharetra. Suspendisse ac venenatis odio. Sed blandit posuere erat a posuere. Suspendisse odio erat, elementum sodales ante vel, malesuada rutrum turpis.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed posuere eget nulla a pharetra. Suspendisse ac venenatis odio. Sed blandit posuere erat a posuere. Suspendisse odio erat, elementum sodales ante vel, malesuada rutrum turpis.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed posuere eget nulla a pharetra. Suspendisse ac venenatis odio. Sed blandit posuere erat a posuere. Suspendisse odio erat, elementum sodales ante vel, malesuada rutrum turpis.
UBC iGEM 2015