Difference between revisions of "Team:METU Turkey/Experiments"
Ilkem.kumru (Talk | contribs) |
Ilkem.kumru (Talk | contribs) |
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Competent cells were prepared | Competent cells were prepared | ||
7 strains | 7 strains | ||
− | Transformation was done at night for control purposes, 5 strains were negative (amp plate 4 min 4000 rpm) | + | Transformation was done at night for control purposes, |
+ | 5 strains were negative (amp plate 4 min 4000 rpm) | ||
July 17th, Friday | July 17th, Friday | ||
Kitten gene was extracted | Kitten gene was extracted | ||
5 strains | 5 strains | ||
− | Transformation was done for control purposes. This was so because 5 strains were negative in the previous one. | + | Transformation was done for control purposes. |
+ | This was so because 5 strains were negative in the previous one. | ||
Kumomax enzyme was planted from agar plate to agar plate. | Kumomax enzyme was planted from agar plate to agar plate. | ||
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Plasmid isolation was done for 101, 106, 117 and GFP | Plasmid isolation was done for 101, 106, 117 and GFP | ||
Restriction was done | Restriction was done | ||
− | Gel electrophoresis was done for the cut 101, 106, 117 and GFP, the results were negative | + | Gel electrophoresis was done for the cut 101, 106, 117 and GFP, |
+ | the results were negative | ||
101, 106, 117 and GFP were again planted in LB from agar | 101, 106, 117 and GFP were again planted in LB from agar | ||
Line 271: | Line 274: | ||
Plasmid isolation was done for 101, 106, 117 and GFP | Plasmid isolation was done for 101, 106, 117 and GFP | ||
Restriction was done for 101, 106, 117 and GFP | Restriction was done for 101, 106, 117 and GFP | ||
− | Gel electrophoresis was done for the cut 101, 106, 117 and GFP, the results were positive only for the promoters, 101, 106, and 117 | + | Gel electrophoresis was done for the cut 101, 106, 117 and GFP, |
+ | the results were positive only for the promoters, 101, 106, and 117 | ||
Gel extraction was done for promoters | Gel extraction was done for promoters | ||
Nanodrop results were low | Nanodrop results were low | ||
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− | The electrophoresis results were acquired for set 2 and set 2, set 1 did not produce results | + | The electrophoresis results were acquired for set 2 and set 2, set 1 |
+ | did not produce results | ||
Gel extraction was done for the 4 bands that yielded positive results | Gel extraction was done for the 4 bands that yielded positive results | ||
Nanodrop results were low | Nanodrop results were low |
Revision as of 20:00, 17 September 2015
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