Difference between revisions of "Team:NU Kazakhstan"

 
(15 intermediate revisions by the same user not shown)
Line 7: Line 7:
 
<div class="work-title">
 
<div class="work-title">
 
<h3>Project Abstract</h3>
 
<h3>Project Abstract</h3>
−
<h4>Prevention of Dental Caries by Targeting Streptococcus Mutans</h4>  
+
<h4>Prevention of Dental Caries by Targeting <i>Streptococcus mutans</i> Mechanisms of Quorum Sensing and Production of Lactic Acid</h4>  
−
<p>Streptococcus mutans is the most widely accepted cause of dental caries. ComX stimulates transcription of genes essential for competence. However, there is a chance to reduce ComX level by overexpressing its novel antagonist, XrpA. A highly expressed level of XrpA protein leads to decreased biofilm formation. Our project aims two goals. The first one is to overexpress the production of XrpA in order to cease the production of ComX protein. The second one will implement CRISPR-dCas9 system in order to block the transcription of VicK gene. VicK is the gene of VicK/VicR signal transduction system in S.mutans that is responsible for acidity tolerance in bacteria. Thus, second system will combine sgRNA for VicK and dCas9 to reduce lactic acid production. Smart systems will be eliminated by blue light safety regulation system that targets origin of replication of the plasmid constructs.
+
<p>Streptococcus mutans is the most widely accepted cause of dental caries. ComX stimulates transcription of genes essential for competence. However, there is a chance to reduce ComX level by overexpressing its novel antagonist, XrpA. A highly expressed level of XrpA protein leads to decreased biofilm formation. Our project aims two goals. The first one is to overexpress the production of XrpA in order to cease the production of ComX protein. The second one will implement CRISPR-dCas9 system in order to block the transcription of VicK gene. VicK is the gene of VicK/VicR signal transduction system in <i>S.mutans</i> that is responsible for acidity tolerance in bacteria. Thus, second system will combine sgRNA for VicK and dCas9 to reduce lactic acid production. Smart systems will be eliminated by blue light safety regulation system that targets origin of replication of the plasmid constructs.
 
 
 +
<hr>
 +
 
</div>
 
</div>
 +
 
 
 
<!-- <div class="col-md-4 work-grids">
 
<!-- <div class="col-md-4 work-grids">
Line 30: Line 33:
 
<li><img src="images/icon4.png" alt=""></li>
 
<li><img src="images/icon4.png" alt=""></li>
 
<li>
 
<li>
−
<h4>Excepturi sint occa</h4>
+
−
<p>praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident</p>
+
 
</li>
 
</li>
 
</ul>
 
</ul>
Line 38: Line 40:
 
</div>
 
</div>
 
</div>
 
</div>
−
<div class="like">
+
−
<div class="container">
+
−
<img src="https://static.igem.org/mediawiki/2015/0/0a/Nazarbayev_uni.jpg" alt="" width="50%">
+
−
</div>
+
 
</div>
 
</div>
 
<div class="footer">
 
<div class="footer">
Line 78: Line 77:
 
<div class="col-md-4 recent-posts">
 
<div class="col-md-4 recent-posts">
 
 
−
<h4>Our address</h4>
+
−
<p class="adrs">Investigationes demonstraverunt lectores legere me lius quod ii legunt saepius. Claritas est etiam processus dynamicus,
+
−
facilisi Nam liber tempor cum soluta </p>
+
−
<ul>
+
−
<li><span></span>Kabanbay batyr Av. 53 Astana, Kazakhstan</li>
+
−
<li><span class="mail"></span><a href="mailto:mail@nu.edu.kz">msadykov@nu.edu.kz</a></li>
+
−
</ul>
+
 
 
 
</div>
 
</div>
Line 107: Line 100:
 
 
 
});
 
});
−
</script>
+
 
−
<a href="#" id="toTop" style="display: block;"> <span id="toTopHover" style="opacity: 1;"> </span></a>
+
−
    <script src="https://2015.igem.org/Template:NU_Kazakhstan/js/bootstrap?action=raw&ctype=text/javascript"> </script>
+
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 00:57, 19 September 2015

Nazarbayev University Team

Project Abstract

Prevention of Dental Caries by Targeting Streptococcus mutans Mechanisms of Quorum Sensing and Production of Lactic Acid

Streptococcus mutans is the most widely accepted cause of dental caries. ComX stimulates transcription of genes essential for competence. However, there is a chance to reduce ComX level by overexpressing its novel antagonist, XrpA. A highly expressed level of XrpA protein leads to decreased biofilm formation. Our project aims two goals. The first one is to overexpress the production of XrpA in order to cease the production of ComX protein. The second one will implement CRISPR-dCas9 system in order to block the transcription of VicK gene. VicK is the gene of VicK/VicR signal transduction system in S.mutans that is responsible for acidity tolerance in bacteria. Thus, second system will combine sgRNA for VicK and dCas9 to reduce lactic acid production. Smart systems will be eliminated by blue light safety regulation system that targets origin of replication of the plasmid constructs.