Project Results
We genetially engienered several strains of E. coli in an effort to increase copper resistance and collection. We named each strain after our school's mascot, the Chesapeake Bay Retriever. Retriever 1 (Ret1) has been given the yeast metallothionein gene CUP1. Retriever 2 (Ret2) has been given the same CUP1 gene codon optimized for E. coli. Each strain was tested for cell density and extracellular copper concentration at different initial copper sulfate concentrations for 7 hours.