Difference between revisions of "Team:UST Beijing/Project"

 
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           <h3>Description</h3>
 
           <h3>Description</h3>
           <p>We know that E.coli divides in LB medium at 37C around 20-30min per cycle.  In this 20-30 minutes, the bacterium has to duplicate its DNA, 4.5 million base pairs, produce every protein of its body another copy, assemble them into structures, and divide into two almost identical copies of individual bacterium.</p>
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           <p>We know that E.coli divides in LB medium at 37℃ around 20-30min per cycle.  In this 20-30 minutes, the bacterium has to duplicate its DNA, 4.5 million base pairs, produce every protein of its body another copy, assemble them into structures, and divide into two almost identical copies of individual bacterium.</p>
 
           <p>On top of this, if we introduce a plasmid of 3kb long, 500 copies of it, into every E.coli individual, we would add some pressure to this replicating process. In this plasmid (pB1A3) , a RFP protein expression cassette (part number BBa_J004450) is inserted.  The promoter has a lacI operon but in DH5alpha strain, its basal expression seems not under tight LacI control, probably due to weak expression levels of LacI in DH5alpha, and plasmid high copy numbers.</p>
 
           <p>On top of this, if we introduce a plasmid of 3kb long, 500 copies of it, into every E.coli individual, we would add some pressure to this replicating process. In this plasmid (pB1A3) , a RFP protein expression cassette (part number BBa_J004450) is inserted.  The promoter has a lacI operon but in DH5alpha strain, its basal expression seems not under tight LacI control, probably due to weak expression levels of LacI in DH5alpha, and plasmid high copy numbers.</p>
 
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         <a href="https://static.igem.org/mediawiki/2015/7/76/UST_Beijing_Pro1.jpg"><img src="https://static.igem.org/mediawiki/2015/7/76/UST_Beijing_Pro1.jpg" width="100%"/></a>
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        <table class=MsoNormalTable  align=center  style="border-collapse:collapse; width:414.8000pt; mso-table-layout-alt:fixed; mso-padding-alt:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; " ><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:0.5000pt solid windowtext; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >1</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:0.5000pt solid windowtext; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >0%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >100%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >2</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >10%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >90%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >3</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >20%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >80%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >4</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >30%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >70%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >5</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >40%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >60%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >6</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >50%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >50%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >7</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >60%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >40%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >8</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >70%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >30%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >9</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >80%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >20%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >10</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >90%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >10%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr><tr><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:0.5000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext; border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >11</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td><td width=276  valign=top  style="width:207.4000pt; padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ; border-left:31.8750pt none rgb(255,255,255); mso-border-left-alt:31.8750pt none rgb(255,255,255); border-right:0.5000pt solid windowtext; mso-border-right-alt:0.5000pt solid windowtext; border-top:none; ; mso-border-top-alt:0.5000pt solid windowtext; border-bottom:0.5000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext; " ><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >100%&nbsp;LB(or&nbsp;trypton,&nbsp;yeast&nbsp;extract),</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p><p class=MsoNormal  style="text-align:center; " ><span style="mso-spacerun:'yes'; font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >0%&nbsp;glutamate</span><span style="font-family:Calibri; mso-fareast-font-family:宋体; font-size:10.5000pt; mso-font-kerning:1.0000pt; " ><o:p></o:p></span></p></td></tr></table>
+
        <img src="https://static.igem.org/mediawiki/2015/c/ca/UST_Bejing_Pro_Table.jpg" height="100%"/>
 
         </div>
 
         </div>
 
       </div>
 
       </div>
Line 11,947: Line 11,947:
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
 
<div class="banner-bottom">
 
<div class="banner-bottom">
 
   <div class="container">
 
   <div class="container">
 
     <div class="banner-bottom-grids">
 
     <div class="banner-bottom-grids">
       <div class="col-md-12 banner-bottom-grid-text">
+
       <div class="col-md-6 banner-bottom-grid-text">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>1</p>
 
           <p>1</p>
Line 11,956: Line 11,957:
 
         </div>
 
         </div>
 
       </div>
 
       </div>
      <div class="clearfix"> </div>
+
       <div class="col-md-6 banner-bottom-grid-text">
    </div>
+
  </div>
+
</div>
+
<div class="banner-bottom">
+
  <div class="container">
+
    <div class="banner-bottom-grids">
+
       <div class="col-md-12 banner-bottom-grid-text">
+
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>2</p>
 
           <p>2</p>
Line 11,976: Line 11,970:
 
   <div class="container">
 
   <div class="container">
 
     <div class="banner-bottom-grids">
 
     <div class="banner-bottom-grids">
       <div class="col-md-12 banner-bottom-grid-text">
+
       <div class="col-md-6 banner-bottom-grid-text">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>3</p>
 
           <p>3</p>
Line 11,982: Line 11,976:
 
         </div>
 
         </div>
 
       </div>
 
       </div>
      <div class="clearfix"> </div>
+
       <div class="col-md-6 banner-bottom-grid-text">
    </div>
+
  </div>
+
</div>
+
<div class="banner-bottom">
+
  <div class="container">
+
    <div class="banner-bottom-grids">
+
       <div class="col-md-12 banner-bottom-grid-text">
+
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>4</p>
 
           <p>4</p>
Line 12,002: Line 11,989:
 
   <div class="container">
 
   <div class="container">
 
     <div class="banner-bottom-grids">
 
     <div class="banner-bottom-grids">
       <div class="col-md-12 banner-bottom-grid-text">
+
       <div class="col-md-6 banner-bottom-grid-text">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>5</p>
 
           <p>5</p>
Line 12,008: Line 11,995:
 
         </div>
 
         </div>
 
       </div>
 
       </div>
      <div class="clearfix"> </div>
+
       <div class="col-md-6 banner-bottom-grid-text">
    </div>
+
  </div>
+
</div>
+
<div class="banner-bottom">
+
  <div class="container">
+
    <div class="banner-bottom-grids">
+
       <div class="col-md-12 banner-bottom-grid-text">
+
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>6</p>
 
           <p>6</p>
Line 12,028: Line 12,008:
 
   <div class="container">
 
   <div class="container">
 
     <div class="banner-bottom-grids">
 
     <div class="banner-bottom-grids">
       <div class="col-md-12 banner-bottom-grid-text">
+
       <div class="col-md-6 banner-bottom-grid-text">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>7</p>
 
           <p>7</p>
Line 12,034: Line 12,014:
 
         </div>
 
         </div>
 
       </div>
 
       </div>
      <div class="clearfix"> </div>
+
       <div class="col-md-6 banner-bottom-grid-text">
    </div>
+
  </div>
+
</div>
+
<div class="banner-bottom">
+
  <div class="container">
+
    <div class="banner-bottom-grids">
+
       <div class="col-md-12 banner-bottom-grid-text">
+
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>8</p>
 
           <p>8</p>
Line 12,054: Line 12,027:
 
   <div class="container">
 
   <div class="container">
 
     <div class="banner-bottom-grids">
 
     <div class="banner-bottom-grids">
       <div class="col-md-12 banner-bottom-grid-text">
+
       <div class="col-md-6 banner-bottom-grid-text">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>9</p>
 
           <p>9</p>
Line 12,060: Line 12,033:
 
         </div>
 
         </div>
 
       </div>
 
       </div>
      <div class="clearfix"> </div>
+
       <div class="col-md-6 banner-bottom-grid-text">
    </div>
+
  </div>
+
</div>
+
<div class="banner-bottom">
+
  <div class="container">
+
    <div class="banner-bottom-grids">
+
       <div class="col-md-12 banner-bottom-grid-text">
+
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>10</p>
 
           <p>10</p>
Line 12,080: Line 12,046:
 
   <div class="container">
 
   <div class="container">
 
     <div class="banner-bottom-grids">
 
     <div class="banner-bottom-grids">
       <div class="col-md-12 banner-bottom-grid-text">
+
       <div class="col-md-6 banner-bottom-grid-text">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <p>11</p>
 
           <p>11</p>
Line 12,090: Line 12,056:
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
 
<div class="banner-bottom">
 
<div class="banner-bottom">
 
   <div class="container">
 
   <div class="container">
Line 12,121: Line 12,088:
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
         <div class="jumbotron banner-bottom-left wow fadeInLeft animated animated" data-wow-delay="0.5s" style="visibility: visible; -webkit-animation: fadeInLeft 0.5s;">
 
           <a href="https://static.igem.org/mediawiki/2015/9/98/UST_Beijing_Pro13.jpg"><img src="https://static.igem.org/mediawiki/2015/9/98/UST_Beijing_Pro13.jpg" width="100%"/></a>
 
           <a href="https://static.igem.org/mediawiki/2015/9/98/UST_Beijing_Pro13.jpg"><img src="https://static.igem.org/mediawiki/2015/9/98/UST_Beijing_Pro13.jpg" width="100%"/></a>
           <a href="https://static.igem.org/mediawiki/2015/9/95/UST_Beijing_Pro14.jpg"><img src="https://static.igem.org/mediawiki/2015/9/95/UST_Beijing_Pro14.jpg" width="100%"/></a>
+
           <a href="https://static.igem.org/mediawiki/2015/9/95/UST_Beijing_Pro14.jpg"><img src="https://static.igem.org/mediawiki/2015/9/95/UST_Beijing_Pro14.jpg" height="100%"/></a>
 
           <img src="https://static.igem.org/mediawiki/2015/a/aa/UST_Beijing_Pro15.png"/ width="50%">
 
           <img src="https://static.igem.org/mediawiki/2015/a/aa/UST_Beijing_Pro15.png"/ width="50%">
 
         </div>
 
         </div>

Latest revision as of 16:07, 18 September 2015