Through to the different color filter light absorption wavelength determination of the curve, select the yellow filter as we do in the design of the instrument used by the filter, for the quantitative determination of red fluorescent protein.
F=KΦI0εbc
Fluorescence of relative strength, related to many factors.
2.
Measurement, by using fluorescence spectrophotometer for sample curve
F=KΦI0(1-e-εbc)
F-fluorescence intensity, K- instrumental constant, Φ-fluorescence quantum yield, ε-The molar extinction coefficient of the sample, b-Optical path length of sample pool, c-sample concentration.
3.
The relationship between red fluorescent protein concentration and the fluorescence intensity, using the instrument designed to mathematical fitting of experimental data.
fitting curveF=228*(1-exp(-0.155*c))+22
fitting curveF=208*(1-exp(-0.0947*c）
4.
The relationship of the sample concentration and slope
