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1.Petri dish

E.coli which carries RFP plasmid grows into red colony.

2.Extracting solution of RFP

Extract RFP from E.coli with TRITON.

3.Ultraviolet absorption curve

Using ultraviolet spectrophotometer to culture medium and to extract the red fluorescent protein to determine the absorbance curve.

4.Fluorescence microscope photographs

Different bacteria liquid medium, and sampling in different time, with dyed SYPR DNA escherichia coli individuals were observed under a fluorescence microscope in different time and red fluorescent protein expression under different culture conditions.

5.Optical filter

Of all sorts of color filter to determine the absorbance curve, finally choose the yellow filter with maximum light absorption

6.Sample container

Use our homemade quantitative measurement of red fluorescent protein LED by extraction of the filter device and yellow red fluorescent protein quantitatively determined.

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University of Science and Technology Beijing No.30, Xueyuan road, Haidian district, Beijing Province, China

Beijing City University No.269, Middle North 4th Ring Road, Haidian district, Beijing Province, China

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