Team:William and Mary

NOISE - W&M iGEM

W&M iGEM 2015

Project Description
Parts
Basic Part
Composite Part
Part Collections
Measurement
Interlab Study
Collaboration
Software
Human Practices
NOISE

Characterization of promoter-derived transcriptional noise in E. coli

What is noise?

Phenotypic variation that can be observed even in genetically identical populations can be, in part, attributed to the stochastic process of gene expression. Our project aims to tease apart the extrinsic and intrinsic components of gene expression noise, and characterize the noise inherent to commonly used promoters in synthetic biology.
Read more on our Project Description page.

Our Parts

In deciding which parts to submit to the iGEM Registry we focused on three main aspects.

First: ensuring our project is as reproducible and extensible as possible. To that end we have submitted all of new composite fluorescent protein parts that we constructed during the project. Second: Making genome integration as straightforward as possible for iGEM teams. In order to accomplish this goal we designed, tested, and validated a new integrator cassette that allows for simple genome integration using either 3A or Gibson Assembly. Third: Increasing the number of tools available for promoter-mediated regulation in synthetic biology. We created and validated an E. coli codon optimized dCas9 variant and a suite of gRNAs to target the most commonly used promoters in iGEM.

Integrator Suites

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Antibiotic Operons

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dCas9s

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gRNAs

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XFPs under various promoters

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G^2

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Measurement & Modeling

talk a lil about stuff we measured/math model here. 4 components of math model (each will have an icon: Data, analysis, another model, case study)

Data

integrated/plasmid

analysis

parameters and stuff.

Another Model

explain from where

Case Study

applying our model to someone else's stuff

Human Practices

Our Human Practices effort was a multi-faceted outreach approach to science literacy, focusing specifically on spreading a basic understanding of synthetic biology to the general public. We collaborated with numerous organizations (logos below) to host nine educational Synthetic Biology workshops for the public (from first graders to adults!) and to implement our original, 24-activity Synthetic Biology curriculum into schools worldwide, to further sustain our efforts for years to come.

Collaboration

W&M iGEM met and exceeded iGEM's collaboration requirements by collaborating with other researchers in four main ways: creating a pen pal program to connect teams with similar projects, participating in the interlab measurement study, interviewing the general public to provide data to future teams about how to communicate synthetic biology, and collaborating on individual research projects with iGEM teams from University of Georgia, University of Maryland, and Cambridge.

Interlab Study

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PenPal Program

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Imaging

Click for details. (stuff w/ other teams goes here)

Team

Conquering noise, one promoter at a time.

References

Notebook

Safety

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