Team:Freiburg/Labjournals/Cellfree/September
Labjournal Cellfree September
Fri, 04.09.
Cell-free Expression of HA-GFP-His6-His6, His10-GFP-Spy and GFP Bielefeld with KK and Promega
(Luisa)
- 50 µl reactions were incubated at 37°C in the platereader (480/520 nm) for 4 hours in a 384 well plate. afterwards the foil covering the plate was removed a spectrum was obtained
- comparison to GFP 1mg/ml dilutions
- plate layout:
| 1 | 2 | 3 | 4 | 5 | |
|---|---|---|---|---|---|
| A | HA-GFP-His6-His6 (KK) | His10-GFP-Spy (KK) | GFP Bielefeld (KK) | no DNA (KK) | air |
| B | HA-GFP-His6-His6 (KK) | His10-GFP-Spy (KK) | GFP Bielefeld (KK) | no DNA (KK) | air |
| C | HA-GFP-His6-His6 (KK) | His10-GFP-Spy (KK) | GFP Bielefeld (KK) | no DNA (KK) | air |
| D | HA-GFP-His6-His6 (PP) | His10-GFP-Spy (PP) | GFP Bielefeld (PP) | no DNA (PP) | air |
| E | HA-GFP-His6-His6 (PP) | His10-GFP-Spy (PP) | GFP Bielefeld (PP) | no DNA (PP) | air |
| F | HA-GFP-His6-His6 (PP) | His10-GFP-Spy (PP) | GFP Bielefeld (PP) | no DNA (PP) | air |
| G | GFP 1:10 | GFP 1:20 | GFP 1:40 | GFP 1:80 | air |
