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Labjournal Surchem April

Experiment 4: GFP concentration measurement

2015.04.15

from http://web.expasy.org/cgi-bin/protparam/protparam:

Number of amino acids: 244
Molecular weight: 27709.1
Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.
Ext. coefficient    22015
Abs 0.1% (=1 g/l)   0.795, assuming all pairs of Cys residues form cystines

Ext. coefficient    21890
Abs 0.1% (=1 g/l)   0.790, assuming all Cys residues are reduced

from http://christoph-leidig.de/tprot.html

62.684 Protein concentration [μM] \\
1.6853363615716557 Protein concentration [mg/ml]