Team:Freiburg/Protocols/Antibiotics

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Antibiotika Stocks

• weight in amount of Antibiotic in respective slvent with great care. Wear gloves, coat and protective mask!
• stir until completely solved
• filter sterile using a .22 sterile filter
• aliquot and store at -20°C
• spectinomycin precipitates after thawing. Vortex thouroughly prior to use

Antibiotic stocks

Ampicillin

Antiobitic	Ampicillin	Chloramphenicol	Kanamycin	Rifampicin	Spectinomycin
Stock concentration	100 mg/ml	30 mg/ml	50 mg/ml	50 mg/ml	100 mg/ml
Solvent	H2O	EtOH	H2O	DMSO	H2O
Vol. aliquots	1 ml	1 ml	1 ml	1 ml	1 ml
Working concentration	100 µg/ml	???	50 µg/ml	50 µg/ml	100 µg/ml

protocols old