Team:Freiburg/Protocols/Bead Regeneration

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Ni-NTA bead regeneration

LP to regenerate Ni-NTA beads after use and elution of bound proteins

material:
duration:

---

• centrifuge beads in Lysis buffer 1 min @ 500 g, 4°C
• discard supernatant
• add 2 mL 0.5 M NaOH per 15 mL Falcon tube
• resuspend beads by flicking against the tube
• incubate 30 min on ice
• centrifuge 1 min @ 500 g, 4°C
• discard supernatant
• resuspend beads im 20 % ethanol by flicking
• store @ 4°C

protocols, protpur