Team:Freiburg/Protocols/Buffers
Buffers and media
Protein-ladder, buffers, LB-Medium
remarks concerning the protocol (author, adapted from etc.)
material: chemicals, used kits, …
duration: … min
| SDS-PAGE | |
|---|---|
| 10x SDS running buffer | 250mM tris base; 2M glycin; 1% SDS (w/v) |
| 4x SDS loading dye | 40% glycerin (w/v); 20% ß-Mercaptoethanol (v/v); 9% SDS (v/v); 250mM Tris (pH 6,8); 0.5% Bromphenolblue (w/v) |
| Coomassie Brilliant blue staining solution | 45% methanol (v/v); 9% acetic acid; 0.2% CBB R-250 (w/v) |
| Destain solution | 40% Methanol (v/v); 7% acetic acid (v/v); 3% glyerin (w/v) |
