Team:Freiburg/Protocols/Halo Wiesmueller

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Halosurface from Wiesmüller

protocol for functionalizing Pditc coated glass slides with halotag ligand

material: HTL with aminogroup, aqua dest.
duration: ca. … min

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1. make PDITC-slides according to PDITC-protocol
2. incubate PDITC slides in Wiesmüllers Halo tag ligand (HTL) solution (200 nM ??) for 10-24 h
3. wash with aqua dest.
4. dry with nitrogengun
5. Storable ??

protocols, surchem