Team:Freiburg/Protocols/Isoprop Precipitation

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Isopropanol precipitation

Precipitation and concentration of plasmid DNA from midi-preps

material: 5M NaCl, Isopropanol, 70% EtOH
duration: 60 min

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1. add 1/10 volume 5M NaCl
2. add 1 volume ice cold isopropanol
3. incubate for 30 min at -20°C
4. centrifuge 30 min, 4°C (pre-cool the centrifuge)
5. wash DNA pellet with 70% ethanol
6. resuspend in H₂O

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