Team:Freiburg/Protocols/TerrificBroth

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Preparation of Teriffic Broth

material: chemicals, used kits, …
duration: 90 min

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Terrific Broth

Component	for 1 litre medium	remarks
Monopotassium phosphate	5.6 g
Potassium phosphate dibasic	28.9 g
yeast extract	10
Thiamine	10 mg	added after sterilisation (filter sterilized)
Glucose (20%)	40ml	added after sterilisation (filter sterilized)

autoclave medium than add filter sterilized Thiamine and Glucose

Store at 4°C

protocols, surchem