Team:Missouri Rolla/Attributions

Missouri cave photographed by Lynn Dieter

ATTRIBUTIONS

Missouri S&T iGEM is entirely student-run as a design team under the Student Design and Experiential Learning Center. All lab work is performed by members trained through our Lab Training Program. Our members chose our project with inspiration from Wagenigen UR 2014 and Toulouse 2014, and our wonderful advisors Dr. Katie Shannon and Dr. David Westenberg are always available for advice.

Our lab received generous financial support from the Biological Sciences, Chemistry, and Chemical Engineering departments at Missouri S&T, and Fred Kielhorn. New England BioLabs and Bio Basic provided us with essential lab supplies, and the free DNA from Integrated DNA Technologies formed an integral part of our project this year.

Our speakers at our White Nose Syndrome Symposium, Shelly Colatskie, Anthony Elliot, Dr. Sarah Hooper, and Dr. Lynn Robbins, made the event possible.

The Crow Lab at Georgia State University kindly offered to test our project on live P. Destructans.

References considered in the design of our project:

  • Beck, Z. Q., Calabria, A. R., Miller, M. C., Vaviline, D. V., Nielsen, A. T. (2013). Increased isoprene production using the archaeal lower mevalonate pathway. US Patent 8361762 B2.
  • Cornelison, C. T., Keel, M. K., Gabriel, K. T., Barlament, C. K., Tucker, T. A., Pierce, G. E., & Crow, S. A. (2014). A preliminary report on the contact-independent antagonism of Pseudogymnoascus destructans by Rhodococcus rhodochrous strain DAP96253. BMC microbiology, 14(1), 246.
  • Cornelison, C. T., Gabriel, K. T., Barlament, C., & Crow Jr, S. A. (2014). Inhibition of Pseudogymnoascus destructans growth from conidia and mycelial extension by bacterially produced volatile organic compounds. Mycopathologia, 177(1-2), 1-10.
  • Hahn, F. M., Hurlburt, A. P., & Poulter, C. D. (1999). Escherichia coli Open Reading Frame 696 Is idi, a Nonessential Gene Encoding Isopentenyl Diphosphate Isomerase. Journal of Bacteriology, 181(15), 4499–4504.
  • Hoyt, J. R., Cheng, T. L., Langwig, K. E., Hee, M. M., Frick, W. F., & Kilpatrick, A. M. (2015). Bacteria isolated from bats inhibit the growth of Pseudogymnoascus destructans, the causative agent of white-nose syndrome.
  • Martin, V. J., Pitera, D. J., Withers, S. T., Newman, J. D., & Keasling, J. D. (2003). Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nature biotechnology, 21(7), 796-802.
  • Matasyoh, L. G., Matasyoh, J. C., Wachira, F. N., Kinyua, M. G., Muigai, A. W. T., & Mukiama, T. K. (2007). Chemical composition and antimicrobial activity of the essential oil of Ocimum gratissimum L. growing in Eastern Kenya. African Journal of Biotechnology, 6(6).
  • Primak, Y. A., Du, M., Miller, M. C., Wells, D. H., Nielsen, A. T., Weyler, W., & Beck, Z. Q. (2011). Characterization of a feedback-resistant mevalonate kinase from the archaeon Methanosarcina mazei. Applied and environmental microbiology, 77(21), 7772-7778.
  • Vickers, C. E., Bongers, M., Liu, Q., Delatte, T., & Bouwmeester, H. (2014). Metabolic engineering of volatile isoprenoids in plants and microbes. Plant, cell & environment, 37(8), 1753-1775.
  • Yoon, S. H., Lee, S. H., Das, A., Ryu, H. K., Jang, H. J., Kim, J. Y., ... & Kim, S. W. (2009). Combinatorial expression of bacterial whole mevalonate pathway for the production of β-carotene in E. coli. Journal of biotechnology, 140(3), 218-226.

Missouri cave photo by Lynn Dieter