Difference between revisions of "Team:UiOslo Norway/Experiments"
Line 24: | Line 24: | ||
<h3>Handling bacteria </h3> | <h3>Handling bacteria </h3> | ||
<ul> | <ul> | ||
− | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/ | + | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/E_coli_strains"><i>E. coli</i> strains </a></li> |
− | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/Transformation | + | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/Transformation">Transformation of chemically <i>E. coli</i> competent cells</a></li> |
− | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/ | + | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/Antibiotics">Concentration of antibiotics</a></li> |
<li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/LB-media">LB-media </a></li> | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/LB-media">LB-media </a></li> | ||
− | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/NMS- | + | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/NMS-medium_M_c(bath)">NMS-medium for growth of <i>Methylococcus capsulatus</i> (Bath)</a></li> |
− | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/ | + | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/NMS medium">Solutions for NMS medium</a></li> |
Revision as of 10:26, 14 August 2015
Protocols
Molecular biology
- PCR with Phusion-HF DNA Polymerase (NEB)
- PCR with Taq DNA Polymerase (NEB)
- Restriction Digest
- Shrimp Alkaline Phosphatase Treatment (Fermentas)
- Ligation with T4 DNA Polymerase (NEB)
- TOPO Zero blunt cloning (Invitrogen)
- Wizard® SV Gel and PCR Clean-Up System (Promega)
- QIAprep Spin Miniprep Kit (Qiagen)
- NucleoSpin® Tissue (Machery-Nagle)
- In vitro Mutagenesis
- Agarose Gel
- List of primers