Difference between revisions of "Team:Edinburgh/Basic Part"

Line 81: Line 81:
  
 
   <div class="container">   
 
   <div class="container">   
 +
<div class="panel panel-default">
 +
  <div class="panel-heading">
 +
    <h3 class="panel-title">Panel title</h3>
 +
  </div>
 +
</div>
 +
    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingOne">
 +
          <h4 class="panel-title">
 +
            <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne">
 +
              1% Agarose Gel
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p style="color: black;">
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1g Agarose
 +
                <li>100ml 1X TAE buffer
 +
                <li>5µl GelRed stain
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p class="text-muted">
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Mix the agarose with the 1X TAE buffer in a flask.
 +
                <li>2. Heat the mixture until all the agarose is dissolved.
 +
                <li>3. Swirl the flask under cold running water to cool the mixture.
 +
                <li> 4. Add the gel stain.
 +
                <li>5. Pour into an assembled gel tray and let it cool.
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingTwo">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo">
 +
            Agarose Gel Electrophoresis
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseTwo" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingTwo">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1% Agarose
 +
                <li>1X TAE buffer
 +
                <li>5X loading dye
 +
                <li>DNA ladder
 +
                <li>DNA samples
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Place gel tray into the electrophoresis apparatus.
 +
                <li>2. Pour 1X TAE so that the gel is covered by buffer.
 +
                <li>3. Prepare the samples by adding the appropriate amount of loading dye.
 +
                <li>4. Load samples and DNA ladder into wells on the gel.
 +
                <li>5. Run the gel at roughly 100V for around an hour
 +
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingThree">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="false" aria-controls="collapseThree">
 +
              Culture
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree">
 +
          <div class="panel-body">
 +
          <div class="col-md-6">
 +
              <p>
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>10ml Luria Broth (LB)
 +
                <li>10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
 +
                <li>Loop (for picking colony)
 +
                <li>Ethanol
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Pour 10ml of LB into a 50ml Falcon tube.
 +
                <li>2. Pipette 10µl of antibiotic into the broth.
 +
                <li>3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
 +
                <li>4. Incubate at 37°C overnight in a shaking incubator.
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingFour">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseFour" aria-expanded="false" aria-controls="collapseFour">
 +
            Gel Purification using QIAquick Gel Extraction Kit
 +
            </a>
 +
          </h4>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
 +
<div class="panel panel-default">
 +
  <div class="panel-heading">
 +
    <h3 class="panel-title">Panel title</h3>
 +
  </div>
 +
</div>
 +
    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingOne">
 +
          <h4 class="panel-title">
 +
            <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne">
 +
              1% Agarose Gel
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p style="color: black;">
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1g Agarose
 +
                <li>100ml 1X TAE buffer
 +
                <li>5µl GelRed stain
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p class="text-muted">
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Mix the agarose with the 1X TAE buffer in a flask.
 +
                <li>2. Heat the mixture until all the agarose is dissolved.
 +
                <li>3. Swirl the flask under cold running water to cool the mixture.
 +
                <li> 4. Add the gel stain.
 +
                <li>5. Pour into an assembled gel tray and let it cool.
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingTwo">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo">
 +
            Agarose Gel Electrophoresis
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseTwo" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingTwo">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1% Agarose
 +
                <li>1X TAE buffer
 +
                <li>5X loading dye
 +
                <li>DNA ladder
 +
                <li>DNA samples
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Place gel tray into the electrophoresis apparatus.
 +
                <li>2. Pour 1X TAE so that the gel is covered by buffer.
 +
                <li>3. Prepare the samples by adding the appropriate amount of loading dye.
 +
                <li>4. Load samples and DNA ladder into wells on the gel.
 +
                <li>5. Run the gel at roughly 100V for around an hour
 +
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingThree">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="false" aria-controls="collapseThree">
 +
              Culture
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree">
 +
          <div class="panel-body">
 +
          <div class="col-md-6">
 +
              <p>
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>10ml Luria Broth (LB)
 +
                <li>10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
 +
                <li>Loop (for picking colony)
 +
                <li>Ethanol
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Pour 10ml of LB into a 50ml Falcon tube.
 +
                <li>2. Pipette 10µl of antibiotic into the broth.
 +
                <li>3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
 +
                <li>4. Incubate at 37°C overnight in a shaking incubator.
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingFour">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseFour" aria-expanded="false" aria-controls="collapseFour">
 +
            Gel Purification using QIAquick Gel Extraction Kit
 +
            </a>
 +
          </h4>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
 +
<div class="panel panel-default">
 +
  <div class="panel-heading">
 +
    <h3 class="panel-title">Panel title</h3>
 +
  </div>
 +
</div>
 +
    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingOne">
 +
          <h4 class="panel-title">
 +
            <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne">
 +
              1% Agarose Gel
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p style="color: black;">
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1g Agarose
 +
                <li>100ml 1X TAE buffer
 +
                <li>5µl GelRed stain
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p class="text-muted">
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Mix the agarose with the 1X TAE buffer in a flask.
 +
                <li>2. Heat the mixture until all the agarose is dissolved.
 +
                <li>3. Swirl the flask under cold running water to cool the mixture.
 +
                <li> 4. Add the gel stain.
 +
                <li>5. Pour into an assembled gel tray and let it cool.
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingTwo">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo">
 +
            Agarose Gel Electrophoresis
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseTwo" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingTwo">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1% Agarose
 +
                <li>1X TAE buffer
 +
                <li>5X loading dye
 +
                <li>DNA ladder
 +
                <li>DNA samples
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Place gel tray into the electrophoresis apparatus.
 +
                <li>2. Pour 1X TAE so that the gel is covered by buffer.
 +
                <li>3. Prepare the samples by adding the appropriate amount of loading dye.
 +
                <li>4. Load samples and DNA ladder into wells on the gel.
 +
                <li>5. Run the gel at roughly 100V for around an hour
 +
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingThree">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="false" aria-controls="collapseThree">
 +
              Culture
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree">
 +
          <div class="panel-body">
 +
          <div class="col-md-6">
 +
              <p>
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>10ml Luria Broth (LB)
 +
                <li>10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
 +
                <li>Loop (for picking colony)
 +
                <li>Ethanol
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Pour 10ml of LB into a 50ml Falcon tube.
 +
                <li>2. Pipette 10µl of antibiotic into the broth.
 +
                <li>3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
 +
                <li>4. Incubate at 37°C overnight in a shaking incubator.
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingFour">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseFour" aria-expanded="false" aria-controls="collapseFour">
 +
            Gel Purification using QIAquick Gel Extraction Kit
 +
            </a>
 +
          </h4>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
 
<div class="panel panel-default">
 
<div class="panel panel-default">
 
   <div class="panel-heading">
 
   <div class="panel-heading">

Revision as of 10:31, 1 September 2015

Basic Parts




Panel title

Materials

  • 1g Agarose
  • 100ml 1X TAE buffer
  • 5µl GelRed stain

Procedure

  • 1. Mix the agarose with the 1X TAE buffer in a flask.
  • 2. Heat the mixture until all the agarose is dissolved.
  • 3. Swirl the flask under cold running water to cool the mixture.
  • 4. Add the gel stain.
  • 5. Pour into an assembled gel tray and let it cool.

Materials

  • 1% Agarose
  • 1X TAE buffer
  • 5X loading dye
  • DNA ladder
  • DNA samples

Procedure

  • 1. Place gel tray into the electrophoresis apparatus.
  • 2. Pour 1X TAE so that the gel is covered by buffer.
  • 3. Prepare the samples by adding the appropriate amount of loading dye.
  • 4. Load samples and DNA ladder into wells on the gel.
  • 5. Run the gel at roughly 100V for around an hour

Materials

  • 10ml Luria Broth (LB)
  • 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
  • Loop (for picking colony)
  • Ethanol

Procedure

  • 1. Pour 10ml of LB into a 50ml Falcon tube.
  • 2. Pipette 10µl of antibiotic into the broth.
  • 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
  • 4. Incubate at 37°C overnight in a shaking incubator.

Panel title

Materials

  • 1g Agarose
  • 100ml 1X TAE buffer
  • 5µl GelRed stain

Procedure

  • 1. Mix the agarose with the 1X TAE buffer in a flask.
  • 2. Heat the mixture until all the agarose is dissolved.
  • 3. Swirl the flask under cold running water to cool the mixture.
  • 4. Add the gel stain.
  • 5. Pour into an assembled gel tray and let it cool.

Materials

  • 1% Agarose
  • 1X TAE buffer
  • 5X loading dye
  • DNA ladder
  • DNA samples

Procedure

  • 1. Place gel tray into the electrophoresis apparatus.
  • 2. Pour 1X TAE so that the gel is covered by buffer.
  • 3. Prepare the samples by adding the appropriate amount of loading dye.
  • 4. Load samples and DNA ladder into wells on the gel.
  • 5. Run the gel at roughly 100V for around an hour

Materials

  • 10ml Luria Broth (LB)
  • 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
  • Loop (for picking colony)
  • Ethanol

Procedure

  • 1. Pour 10ml of LB into a 50ml Falcon tube.
  • 2. Pipette 10µl of antibiotic into the broth.
  • 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
  • 4. Incubate at 37°C overnight in a shaking incubator.

Panel title

Materials

  • 1g Agarose
  • 100ml 1X TAE buffer
  • 5µl GelRed stain

Procedure

  • 1. Mix the agarose with the 1X TAE buffer in a flask.
  • 2. Heat the mixture until all the agarose is dissolved.
  • 3. Swirl the flask under cold running water to cool the mixture.
  • 4. Add the gel stain.
  • 5. Pour into an assembled gel tray and let it cool.

Materials

  • 1% Agarose
  • 1X TAE buffer
  • 5X loading dye
  • DNA ladder
  • DNA samples

Procedure

  • 1. Place gel tray into the electrophoresis apparatus.
  • 2. Pour 1X TAE so that the gel is covered by buffer.
  • 3. Prepare the samples by adding the appropriate amount of loading dye.
  • 4. Load samples and DNA ladder into wells on the gel.
  • 5. Run the gel at roughly 100V for around an hour

Materials

  • 10ml Luria Broth (LB)
  • 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
  • Loop (for picking colony)
  • Ethanol

Procedure

  • 1. Pour 10ml of LB into a 50ml Falcon tube.
  • 2. Pipette 10µl of antibiotic into the broth.
  • 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
  • 4. Incubate at 37°C overnight in a shaking incubator.

Panel title

Materials

  • 1g Agarose
  • 100ml 1X TAE buffer
  • 5µl GelRed stain

Procedure

  • 1. Mix the agarose with the 1X TAE buffer in a flask.
  • 2. Heat the mixture until all the agarose is dissolved.
  • 3. Swirl the flask under cold running water to cool the mixture.
  • 4. Add the gel stain.
  • 5. Pour into an assembled gel tray and let it cool.

Materials

  • 1% Agarose
  • 1X TAE buffer
  • 5X loading dye
  • DNA ladder
  • DNA samples

Procedure

  • 1. Place gel tray into the electrophoresis apparatus.
  • 2. Pour 1X TAE so that the gel is covered by buffer.
  • 3. Prepare the samples by adding the appropriate amount of loading dye.
  • 4. Load samples and DNA ladder into wells on the gel.
  • 5. Run the gel at roughly 100V for around an hour

Materials

  • 10ml Luria Broth (LB)
  • 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
  • Loop (for picking colony)
  • Ethanol

Procedure

  • 1. Pour 10ml of LB into a 50ml Falcon tube.
  • 2. Pipette 10µl of antibiotic into the broth.
  • 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
  • 4. Incubate at 37°C overnight in a shaking incubator.