Difference between revisions of "Team:Edinburgh/Composite Part"

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{{Edinburgh}}
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{{Edinburgh_practices}}
 
<html>
 
<html>
  
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<h2> Composite Parts</h2>
 
  
 +
    <head>
 +
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/css/bootstrap.min.css">
 +
<script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/js/bootstrap.min.js"></script>
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    </head>
  
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<div class="highlightBox">
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<body>
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<h4>Note</h4>
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−
<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best New Composite Part award</a>, you must fill out this page. Please give links to the Registry entries for the Composite parts you have made. Please see the Registry's <a href="http://parts.igem.org/Help:Parts#Basic_and_Composite_Parts"> Help:Parts page</a> for more information on part types.</p>
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                  <a href="https://2015.igem.org/Team:Edinburgh">Home</a></li>
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                      <li><a href="https://2015.igem.org/Team:Edinburgh/Description">Description</a></li>
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                      <li><a href="https://2015.igem.org/Team:Edinburgh/Experiments">Experiments</a> </li>
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                      <li><a href="https://2015.igem.org/Team:Edinburgh/Project/Protocols">Protocols</a> </li>
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                      <li><a href="https://2015.igem.org/Team:Edinburgh/Results">Results</a></li>
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                      <li><a href="https://2015.igem.org/Team:Edinburgh/Design">Design</a></li>
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                      <li><a href="https://2015.igem.org/Team:Edinburgh/Parts">Team Parts</a></li>
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                      <li><a href="https://2015.igem.org/Team:Edinburgh/Basic_Part">Basic Parts</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:Edinburgh/Composite_Part">Composite Parts</a></li>
 +
                      <li><a href="https://2015.igem.org/Team:Edinburgh/Part_Collection">Part Collection</a> </li>
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                    </ul>
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                  </li>
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                  <li><a href="https://2015.igem.org/Team:Edinburgh/Attributions">Attributions</a></li>
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                  <li><a href="https://2015.igem.org/Team:Edinburgh/Practices">Policy and Practices</a></li>
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                  <li><a href="https://2015.igem.org/Team:Edinburgh/Safety">Safety</a></li>
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                  <li><a href="https://2015.igem.org/Team:Edinburgh/Modeling">Modeling</a></li>
 +
                  <li><a href="https://2015.igem.org/Team:Edinburgh/InterLab">InterLab</a></li>
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                  <li><a href="software.html">Software</a></li>
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                  <li><a href="entrepreneurship.html">Entrepreneurship</a></li>
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                  <li><a href="collaborations.html">Collaborations</a></li>
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 +
 
 +
        <header class="intro">
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        <div class="intro-body">
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          <div class="container">
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            <div class="row">
 +
              <div class="col-md-12">
 +
                <h1 class="brand-heading">Basic Parts</h1>
 +
                <p class="intro-text">
 +
<br>
 +
<br>
 +
<br>
 +
                </p>
 +
              </div>
 +
            </div>
 +
          </div>
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        </div>
 +
      </header>
 +
 
 +
 
 +
  <div class="container"> 
 +
<div class="panel panel-default">
 +
  <div class="panel-heading">
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    <h3 class="panel-title">Panel title</h3>
 +
  </div>
 
</div>
 
</div>
 +
    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingOne">
 +
          <h4 class="panel-title">
 +
            <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne">
 +
              1% Agarose Gel
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p style="color: black;">
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1g Agarose
 +
                <li>100ml 1X TAE buffer
 +
                <li>5µl GelRed stain
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p class="text-muted">
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Mix the agarose with the 1X TAE buffer in a flask.
 +
                <li>2. Heat the mixture until all the agarose is dissolved.
 +
                <li>3. Swirl the flask under cold running water to cool the mixture.
 +
                <li> 4. Add the gel stain.
 +
                <li>5. Pour into an assembled gel tray and let it cool.
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingTwo">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo">
 +
            Agarose Gel Electrophoresis
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseTwo" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingTwo">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1% Agarose
 +
                <li>1X TAE buffer
 +
                <li>5X loading dye
 +
                <li>DNA ladder
 +
                <li>DNA samples
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Place gel tray into the electrophoresis apparatus.
 +
                <li>2. Pour 1X TAE so that the gel is covered by buffer.
 +
                <li>3. Prepare the samples by adding the appropriate amount of loading dye.
 +
                <li>4. Load samples and DNA ladder into wells on the gel.
 +
                <li>5. Run the gel at roughly 100V for around an hour
  
−
<p>
+
              </uL>
−
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
+
            </p>
−
</p>
+
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingThree">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="false" aria-controls="collapseThree">
 +
              Culture
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree">
 +
          <div class="panel-body">
 +
          <div class="col-md-6">
 +
              <p>
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>10ml Luria Broth (LB)
 +
                <li>10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
 +
                <li>Loop (for picking colony)
 +
                <li>Ethanol
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Pour 10ml of LB into a 50ml Falcon tube.
 +
                <li>2. Pipette 10µl of antibiotic into the broth.
 +
                <li>3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
 +
                <li>4. Incubate at 37°C overnight in a shaking incubator.
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
  
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
+
<div class="panel panel-default">
 +
  <div class="panel-heading">
 +
    <h3 class="panel-title">Panel title</h3>
 +
  </div>
 +
</div>
 +
    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingOne">
 +
          <h4 class="panel-title">
 +
            <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne">
 +
              1% Agarose Gel
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p style="color: black;">
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1g Agarose
 +
                <li>100ml 1X TAE buffer
 +
                <li>5µl GelRed stain
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p class="text-muted">
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Mix the agarose with the 1X TAE buffer in a flask.
 +
                <li>2. Heat the mixture until all the agarose is dissolved.
 +
                <li>3. Swirl the flask under cold running water to cool the mixture.
 +
                <li> 4. Add the gel stain.
 +
                <li>5. Pour into an assembled gel tray and let it cool.
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingTwo">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo">
 +
            Agarose Gel Electrophoresis
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseTwo" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingTwo">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1% Agarose
 +
                <li>1X TAE buffer
 +
                <li>5X loading dye
 +
                <li>DNA ladder
 +
                <li>DNA samples
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Place gel tray into the electrophoresis apparatus.
 +
                <li>2. Pour 1X TAE so that the gel is covered by buffer.
 +
                <li>3. Prepare the samples by adding the appropriate amount of loading dye.
 +
                <li>4. Load samples and DNA ladder into wells on the gel.
 +
                <li>5. Run the gel at roughly 100V for around an hour
  
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingThree">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="false" aria-controls="collapseThree">
 +
              Culture
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree">
 +
          <div class="panel-body">
 +
          <div class="col-md-6">
 +
              <p>
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>10ml Luria Broth (LB)
 +
                <li>10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
 +
                <li>Loop (for picking colony)
 +
                <li>Ethanol
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p>
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Pour 10ml of LB into a 50ml Falcon tube.
 +
                <li>2. Pipette 10µl of antibiotic into the broth.
 +
                <li>3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
 +
                <li>4. Incubate at 37°C overnight in a shaking incubator.
 +
              </uL>
 +
            </p>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingFour">
 +
          <h4 class="panel-title">
 +
            <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseFour" aria-expanded="false" aria-controls="collapseFour">
 +
            Gel Purification using QIAquick Gel Extraction Kit
 +
            </a>
 +
          </h4>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
  
 +
<div class="panel panel-default">
 +
  <div class="panel-heading">
 +
    <h3 class="panel-title">Panel title</h3>
 +
  </div>
 
</div>
 
</div>
 +
    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
      <div class="panel panel-default">
 +
        <div class="panel-heading" role="tab" id="headingOne">
 +
          <h4 class="panel-title">
 +
            <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne">
 +
              1% Agarose Gel
 +
            </a>
 +
          </h4>
 +
        </div>
 +
        <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne">
 +
          <div class="panel-body">
 +
            <div class="col-md-6">
 +
              <p style="color: black;">
 +
              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1g Agarose
 +
                <li>100ml 1X TAE buffer
 +
                <li>5µl GelRed stain
 +
              </ul>
 +
            </p>
 +
            </div>
 +
            <div class="col-md-6">
 +
              <p class="text-muted">
 +
              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Mix the agarose with the 1X TAE buffer in a flask.
 +
                <li>2. Heat the mixture until all the agarose is dissolved.
 +
                <li>3. Swirl the flask under cold running water to cool the mixture.
 +
                <li> 4. Add the gel stain.
 +
                <li>5. Pour into an assembled gel tray and let it cool.
 +
              </uL>
 +
            </p>
 +
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            Agarose Gel Electrophoresis
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            <div class="col-md-6">
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              <h2>Materials</h2>
 +
              <ul>
 +
                <li>1% Agarose
 +
                <li>1X TAE buffer
 +
                <li>5X loading dye
 +
                <li>DNA ladder
 +
                <li>DNA samples
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              </ul>
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            <div class="col-md-6">
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              <p>
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              <h2>Procedure</h2>
 +
              <ul>
 +
                <li>1. Place gel tray into the electrophoresis apparatus.
 +
                <li>2. Pour 1X TAE so that the gel is covered by buffer.
 +
                <li>3. Prepare the samples by adding the appropriate amount of loading dye.
 +
                <li>4. Load samples and DNA ladder into wells on the gel.
 +
                <li>5. Run the gel at roughly 100V for around an hour
 +
 +
              </uL>
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              <h2>Materials</h2>
 +
              <ul>
 +
                <li>10ml Luria Broth (LB)
 +
                <li>10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
 +
                <li>Loop (for picking colony)
 +
                <li>Ethanol
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              <h2>Procedure</h2>
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              <ul>
 +
                <li>1. Pour 10ml of LB into a 50ml Falcon tube.
 +
                <li>2. Pipette 10µl of antibiotic into the broth.
 +
                <li>3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
 +
                <li>4. Incubate at 37°C overnight in a shaking incubator.
 +
              </uL>
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            Gel Purification using QIAquick Gel Extraction Kit
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Revision as of 10:42, 1 September 2015

Basic Parts




Panel title

Materials

  • 1g Agarose
  • 100ml 1X TAE buffer
  • 5µl GelRed stain

Procedure

  • 1. Mix the agarose with the 1X TAE buffer in a flask.
  • 2. Heat the mixture until all the agarose is dissolved.
  • 3. Swirl the flask under cold running water to cool the mixture.
  • 4. Add the gel stain.
  • 5. Pour into an assembled gel tray and let it cool.

Materials

  • 1% Agarose
  • 1X TAE buffer
  • 5X loading dye
  • DNA ladder
  • DNA samples

Procedure

  • 1. Place gel tray into the electrophoresis apparatus.
  • 2. Pour 1X TAE so that the gel is covered by buffer.
  • 3. Prepare the samples by adding the appropriate amount of loading dye.
  • 4. Load samples and DNA ladder into wells on the gel.
  • 5. Run the gel at roughly 100V for around an hour

Materials

  • 10ml Luria Broth (LB)
  • 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
  • Loop (for picking colony)
  • Ethanol

Procedure

  • 1. Pour 10ml of LB into a 50ml Falcon tube.
  • 2. Pipette 10µl of antibiotic into the broth.
  • 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
  • 4. Incubate at 37°C overnight in a shaking incubator.

Panel title

Materials

  • 1g Agarose
  • 100ml 1X TAE buffer
  • 5µl GelRed stain

Procedure

  • 1. Mix the agarose with the 1X TAE buffer in a flask.
  • 2. Heat the mixture until all the agarose is dissolved.
  • 3. Swirl the flask under cold running water to cool the mixture.
  • 4. Add the gel stain.
  • 5. Pour into an assembled gel tray and let it cool.

Materials

  • 1% Agarose
  • 1X TAE buffer
  • 5X loading dye
  • DNA ladder
  • DNA samples

Procedure

  • 1. Place gel tray into the electrophoresis apparatus.
  • 2. Pour 1X TAE so that the gel is covered by buffer.
  • 3. Prepare the samples by adding the appropriate amount of loading dye.
  • 4. Load samples and DNA ladder into wells on the gel.
  • 5. Run the gel at roughly 100V for around an hour

Materials

  • 10ml Luria Broth (LB)
  • 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
  • Loop (for picking colony)
  • Ethanol

Procedure

  • 1. Pour 10ml of LB into a 50ml Falcon tube.
  • 2. Pipette 10µl of antibiotic into the broth.
  • 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
  • 4. Incubate at 37°C overnight in a shaking incubator.

Panel title

Materials

  • 1g Agarose
  • 100ml 1X TAE buffer
  • 5µl GelRed stain

Procedure

  • 1. Mix the agarose with the 1X TAE buffer in a flask.
  • 2. Heat the mixture until all the agarose is dissolved.
  • 3. Swirl the flask under cold running water to cool the mixture.
  • 4. Add the gel stain.
  • 5. Pour into an assembled gel tray and let it cool.

Materials

  • 1% Agarose
  • 1X TAE buffer
  • 5X loading dye
  • DNA ladder
  • DNA samples

Procedure

  • 1. Place gel tray into the electrophoresis apparatus.
  • 2. Pour 1X TAE so that the gel is covered by buffer.
  • 3. Prepare the samples by adding the appropriate amount of loading dye.
  • 4. Load samples and DNA ladder into wells on the gel.
  • 5. Run the gel at roughly 100V for around an hour

Materials

  • 10ml Luria Broth (LB)
  • 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
  • Loop (for picking colony)
  • Ethanol

Procedure

  • 1. Pour 10ml of LB into a 50ml Falcon tube.
  • 2. Pipette 10µl of antibiotic into the broth.
  • 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
  • 4. Incubate at 37°C overnight in a shaking incubator.