Difference between revisions of "Team:Freiburg/Results"

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<div class="background_image_left"><img src="https://static.igem.org/mediawiki/2015/8/85/Freiburg_wiki_background_2_parted_left.png"></div>
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<h2> Results </h2>
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We measured the binding of anti-GFP to cell-free expressed GFP in blood serum
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<p>We expressed GFP with our own cell-free expression system and bound it to the surface of our DiaChip. We than ran some rabbit blood serum over the chip which we had spiked with anti-GFP antibodies. The results show that our cell-free proteins can be measured even in a complex solution such as blood serum. </p>
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<img src="https://static.igem.org/mediawiki/2015/7/74/Freiburg_labjournal-roi_selection_20150817_429.png"></img>
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We built our very own, low-cost DiaChip device - and can measure with it!
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<img src="https://static.igem.org/mediawiki/2015/7/74/Freiburg_labjournal-roi_selection_20150817_429.png"></img>
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<p>A green LED, two lenses and a regular SLR Camera is basically all you need to build your own iRIf device. </p>
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Revision as of 00:15, 3 September 2015

""

We successfully diagnosed anti-Tetanus antibodies in human blood serum
Check out this video showing how the antibodies in the serum bind our tetanus antigens on the DiaChip

We took a persons blood before and after a tetanus vaccination and screened for a tetanus infection.

An immunized person has antibodies against tetanus antigens - we bound spots of our purified tetanus antigens onto the specific surface of our chip. We then incubated the DiaChip with 33 µl of the blood serum for half an hour and measured the results.

Take a look at the video and see for yourself how good our DiaChip performed.

And remember: that this isn’t an ELISA, we don’t need no expensive secondary Antibodies!

We measured the binding of anti-GFP to cell-free expressed GFP in blood serum

We expressed GFP with our own cell-free expression system and bound it to the surface of our DiaChip. We than ran some rabbit blood serum over the chip which we had spiked with anti-GFP antibodies. The results show that our cell-free proteins can be measured even in a complex solution such as blood serum.

We built our very own, low-cost DiaChip device - and can measure with it!

A green LED, two lenses and a regular SLR Camera is basically all you need to build your own iRIf device.