Difference between revisions of "Team:Freiburg/Collaborations"

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<h2> Collaborations</h2>
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<h1 class="sectionedit1">Collaboration with iGEM team Bielefeld</h1>
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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<strong>Bielefeld</strong> sends a plasmid based on BBa_I746909 that has a translation enhancing sequence (5’-UTR), and <strong>Freiburg</strong> sends a plasmid containing turboYFP, a His- and a Halo-Tag. We would like to compare if these parts work in different cell-free proteins synthesis environments.
 
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<a class="media" href="https://static.igem.org/mediawiki/2015/f/f8/Freiburg_bielefeld_collab.jpg" title="bielefeld_collab.jpg"><img align="right" alt="" class="mediaright" src="https://static.igem.org/mediawiki/2015/f/f8/Freiburg_bielefeld_collab.jpg" width="200"/></a>
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<h4> Which other teams can we work with? </h4>
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You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the <a hreef="https://2015.igem.org/Judging/Awards#Medals">iGEM team collaboration gold medal criterion</a>.
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In order to meet the gold medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
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To test the plasmid BBa_I746909 containing a translation enhancing sequence (5'-UTR) we compared it to our GFPs used for cell-free expression (HA-GFP-His6-His6 and His-GFP-Spy).
 
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Here are some suggestions for projects you could work on with other teams:
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Both plasmids were treated alike and compared to a sample containing no DNA (negative control) and a dilution series of expressed and purified GFP (positive control). All reactions were performed in triplicates.
 
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The samples were expressed for 2 hours at 37°C in a 384-well plate using our own lysate and premix. After expression, a western blot and dot blot were performed.
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<li> Improve the function of another team's BioBrick Part or Device</li>
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<li> Characterize another team's part </li>
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<li> Model or simulating another team's system </li>
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<li> Test another team's software</li>
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<li> Help build and test another team's hardware project</li>
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<li> Mentor a high-school team</li>
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Revision as of 19:40, 3 September 2015

""

Collaboration with iGEM team Bielefeld

Bielefeld sends a plasmid based on BBa_I746909 that has a translation enhancing sequence (5’-UTR), and Freiburg sends a plasmid containing turboYFP, a His- and a Halo-Tag. We would like to compare if these parts work in different cell-free proteins synthesis environments.

To test the plasmid BBa_I746909 containing a translation enhancing sequence (5'-UTR) we compared it to our GFPs used for cell-free expression (HA-GFP-His6-His6 and His-GFP-Spy).

Both plasmids were treated alike and compared to a sample containing no DNA (negative control) and a dilution series of expressed and purified GFP (positive control). All reactions were performed in triplicates.

The samples were expressed for 2 hours at 37°C in a 384-well plate using our own lysate and premix. After expression, a western blot and dot blot were performed.