Difference between revisions of "Team:Freiburg/Project/pRIG15 8"
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| − | Infection with the Herpes Simplex Virus 1 leads to life-long persistence of the virus inside the body as a latent infection. <sup><a class="fn_top" href="#fn__1" id="fnt__1" name="fnt__1">1)</a></sup> The glycoprotein G is one of 11 glycoproteins expressed by HSV1. <sup><a class="fn_top" href="#fn__2" id="fnt__2" name="fnt__2">2)</a></sup> The sequence we used for our experiment was obtained from | + | |
| + | Infection with the <a class="wikilink1" href="https://2015.igem.org/Team:Freiburg/Project/Diseases#Herpes" title="Herpes simplex">Herpes Simplex Virus 1</a> | ||
| + | leads to life-long persistence of the virus inside the body as a latent infection. <sup><a class="fn_top" href="#fn__1" id="fnt__1" name="fnt__1">1)</a></sup> The glycoprotein G is one of 11 glycoproteins expressed by HSV1. <sup><a class="fn_top" href="#fn__2" id="fnt__2" name="fnt__2">2)</a></sup> The sequence we used for our experiment was obtained from | ||
Jaaskelainen et al., 2009. <sup><a class="fn_top" href="#fn__3" id="fnt__3" name="fnt__3">3)</a></sup> | Jaaskelainen et al., 2009. <sup><a class="fn_top" href="#fn__3" id="fnt__3" name="fnt__3">3)</a></sup> | ||
</p> | </p> | ||
Revision as of 14:23, 6 September 2015
pRIG15_8
Infection with the Herpes Simplex Virus 1 leads to life-long persistence of the virus inside the body as a latent infection. 1) The glycoprotein G is one of 11 glycoproteins expressed by HSV1. 2) The sequence we used for our experiment was obtained from Jaaskelainen et al., 2009. 3)
To insert the sequence for HSV1 glycoprotein G into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. This fragment was amplified via PCR (Link zum Labjournal-Eintrag) and then assembled with the digested pSB1C3 backbone using Gibson assembly. To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing.
