Difference between revisions of "Team:Freiburg/Project/pRIG15 7"
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| − | The Varicella Zoster Virus also called Human Herpes Virus Type 3 causes chickenpox after first infection and remains as a latent infection. The sequence we used during our project codes for the extracellular part of the glycoprotein E <sup><a class="fn_top" href="#fn__1" id="fnt__1" name="fnt__1">1)</a></sup>, which is the most abundant glycoprotein on the surface of infected cells. <sup><a class="fn_top" href="#fn__2" id="fnt__2" name="fnt__2">2)</a></sup> | + | The Varicella Zoster Virus also called Human Herpes Virus Type 3 causes <a class="wikilink1" href="https://2015.igem.org/Team:Freiburg/Project/Diseases#Zoster" title="Varicella">chickenpox</a> after first infection and remains as a latent infection. The sequence we used during our project codes for the extracellular part of the glycoprotein E <sup><a class="fn_top" href="#fn__1" id="fnt__1" name="fnt__1">1)</a></sup>, which is the most abundant glycoprotein on the surface of infected cells. <sup><a class="fn_top" href="#fn__2" id="fnt__2" name="fnt__2">2)</a></sup> |
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Revision as of 15:02, 6 September 2015
pRIG15_7
The Varicella Zoster Virus also called Human Herpes Virus Type 3 causes chickenpox after first infection and remains as a latent infection. The sequence we used during our project codes for the extracellular part of the glycoprotein E 1), which is the most abundant glycoprotein on the surface of infected cells. 2)
To insert the sequence for Varicella Zoster glycoprotein E into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. This fragment was amplified via PCR (Link zum Labjournal-Eintrag) and then assembled with the digested pSB1C3 backbone using Gibson assembly. To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing.
