Difference between revisions of "Team:EPF Lausanne/Part Collection"
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<h1><b>dCas9-ω</h1> | <h1><b>dCas9-ω</h1> | ||
| − | <p>dCa9s-ω is our main tool to build our project in <i>E. coli</i>. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.</p> | + | <p>dCa9s-ω is our main tool to build our project in <i>E. coli</i>. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.</p> |
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| + | <div class="row"> | ||
| + | <div class="col-md-3"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/1/1b/EPF_Lausanne_Parts_J23117.png" style="width:75%"> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-md-6"> | ||
| + | <h1><b>PAM rich URS J23117 promoter</h1> | ||
| + | <p>This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream reguéatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.</p> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-md-3 col-centered"> | ||
| + | <a href="?"><p><b>?</b> </p></a> | ||
| + | </div> | ||
| + | </div> | ||
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</body> | </body> | ||
Revision as of 06:52, 7 September 2015
dCas9-ω
dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.
PAM rich URS J23117 promoter
This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream reguéatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.
