Difference between revisions of "Team:Freiburg/InterLab Study/Details"
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<p> | <p> | ||
All data are provided in arbitrary units and were converted to absolute units using the fluorescein standard curve indicated below. | All data are provided in arbitrary units and were converted to absolute units using the fluorescein standard curve indicated below. | ||
| − | + | <div class="wholetable"> | |
<h2> Top10, 10°C </h2> | <h2> Top10, 10°C </h2> | ||
| + | <p> Raw-data from the measurements of Top10 <i> E. coli </i> cells after incubation for 8h at 37°C and 8h at 10°C. Data are provided in relative fluorescence units as returned by the plate reader device. </p> | ||
<table class="tableizer-table"> | <table class="tableizer-table"> | ||
<tr class="tableizer-firstrow"><th></th><th>biol 1</th><th> </th><th> </th><th>biol 2</th><th> </th><th> </th><th>biol 3</th><th> </th><th> </th></tr> | <tr class="tableizer-firstrow"><th></th><th>biol 1</th><th> </th><th> </th><th>biol 2</th><th> </th><th> </th><th>biol 3</th><th> </th><th> </th></tr> | ||
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<tr><td>BBa_I20270 (pos)</td><td>8662</td><td>8091</td><td>8376</td><td>9430</td><td>9292</td><td>8647</td><td>9335</td><td>9012</td><td>9001</td></tr> | <tr><td>BBa_I20270 (pos)</td><td>8662</td><td>8091</td><td>8376</td><td>9430</td><td>9292</td><td>8647</td><td>9335</td><td>9012</td><td>9001</td></tr> | ||
</table> | </table> | ||
| + | </div> | ||
| + | <div class="wholetable"> | ||
<h2>Top10, 37°C</h2> | <h2>Top10, 37°C</h2> | ||
| + | <p> Raw-data from the measurements of Top10 <i> E. coli </i> cells after incubation for 16h at 37°C. Data are provided in relative fluorescence units as returned by the plate reader device. </p> | ||
<table class="tableizer-table"> | <table class="tableizer-table"> | ||
<tr class="tableizer-firstrow"><th></th><th>biol 1</th><th> </th><th> </th><th>biol 2</th><th> </th><th> </th><th>biol 3</th><th> </th><th> </th></tr> | <tr class="tableizer-firstrow"><th></th><th>biol 1</th><th> </th><th> </th><th>biol 2</th><th> </th><th> </th><th>biol 3</th><th> </th><th> </th></tr> | ||
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<tr><td>BBa_I20270 (pos)</td><td>8518</td><td>8142</td><td>7977</td><td>7958</td><td>8317</td><td>7874</td><td>8506</td><td>8212</td><td>8800</td></tr> | <tr><td>BBa_I20270 (pos)</td><td>8518</td><td>8142</td><td>7977</td><td>7958</td><td>8317</td><td>7874</td><td>8506</td><td>8212</td><td>8800</td></tr> | ||
</table> | </table> | ||
| + | </div> | ||
| + | <div class="wholetable"> | ||
<h2>ArcticExpress, 10°C </h2> | <h2>ArcticExpress, 10°C </h2> | ||
| + | <p> Raw-data from the measurements of Arctic Express <i> E. coli </i> cells after incubation for 8h at 37°C and 8h at 10°C. Data are provided in relative fluorescence units as returned by the plate reader device. </p> | ||
<table class="tableizer-table"> | <table class="tableizer-table"> | ||
<tr class="tableizer-firstrow"><th></th><th>biol 1</th><th> </th><th> </th><th>biol 2</th><th> </th><th> </th><th>biol 3</th><th> </th><th> </th></tr> | <tr class="tableizer-firstrow"><th></th><th>biol 1</th><th> </th><th> </th><th>biol 2</th><th> </th><th> </th><th>biol 3</th><th> </th><th> </th></tr> | ||
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<tr><td>BBa_I20270 (pos)</td><td>14673</td><td>13975</td><td>13659</td><td>13511</td><td>12666</td><td>13579</td><td>13155</td><td>11919</td><td>13791</td></tr> | <tr><td>BBa_I20270 (pos)</td><td>14673</td><td>13975</td><td>13659</td><td>13511</td><td>12666</td><td>13579</td><td>13155</td><td>11919</td><td>13791</td></tr> | ||
</table> | </table> | ||
| + | </div> | ||
| + | <div class="wholetable"> | ||
<h2> ArcticExpress, 37°C </h2> | <h2> ArcticExpress, 37°C </h2> | ||
| + | <p> Raw-data from the measurements of Top10 <i> E. coli </i> cells after incubation for 16h at 37°C. Data are provided in relative fluorescence units as returned by the plate reader device. </p> | ||
<table class="tableizer-table"> | <table class="tableizer-table"> | ||
<tr class="tableizer-firstrow"><th></th><th>biol 1</th><th> </th><th> </th><th>biol 2</th><th> </th><th> </th><th>biol 3</th><th> </th><th> </th></tr> | <tr class="tableizer-firstrow"><th></th><th>biol 1</th><th> </th><th> </th><th>biol 2</th><th> </th><th> </th><th>biol 3</th><th> </th><th> </th></tr> | ||
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<tr><td>BBa_I20270 (pos)</td><td>14000</td><td>12792</td><td>12803</td><td>14657</td><td>14546</td><td>14124</td><td>13238</td><td>12286</td><td>12489</td></tr> | <tr><td>BBa_I20270 (pos)</td><td>14000</td><td>12792</td><td>12803</td><td>14657</td><td>14546</td><td>14124</td><td>13238</td><td>12286</td><td>12489</td></tr> | ||
</table> | </table> | ||
| + | </div> | ||
| + | <div class="wholetable"> | ||
<h2> Fluorescein standard curve </h2> | <h2> Fluorescein standard curve </h2> | ||
| − | The data points for 50 ng fluorescein were excluded from standard curve calculation. | + | <p>The data points for 50 ng fluorescein were excluded from standard curve calculation.</p> |
<table class="tableizer-table"> | <table class="tableizer-table"> | ||
<tr class="tableizer-firstrow"><th>ng abs</th><th>50</th><th>37,5</th><th>25</th><th>12,5</th><th>5</th><th>2,5</th><th>1</th><th>0,5</th><th>0</th></tr> | <tr class="tableizer-firstrow"><th>ng abs</th><th>50</th><th>37,5</th><th>25</th><th>12,5</th><th>5</th><th>2,5</th><th>1</th><th>0,5</th><th>0</th></tr> | ||
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<tr><td></td><td>OVRFLW</td><td>76097</td><td>55356</td><td>28416</td><td>12539</td><td>6672</td><td>2737</td><td>2955</td><td>110</td></tr> | <tr><td></td><td>OVRFLW</td><td>76097</td><td>55356</td><td>28416</td><td>12539</td><td>6672</td><td>2737</td><td>2955</td><td>110</td></tr> | ||
</table> | </table> | ||
| + | </div> | ||
| − | + | <div class="flexbox"> | |
| + | <div class="thumb2 tleft" style="width:610px"><div class="thumbinner"><a class="media" href="https://static.igem.org/mediawiki/2015/d/d4/Freiburg_20150907_standard_curve.png" title="20150907_standard_curve.png"><img alt="" class="mediabox2" src="https://static.igem.org/mediawiki/2015/d/d4/Freiburg_20150907_standard_curve.png" width="600"/></a><div class="thumbcaption"><div class="magnify"><a class="internal" href="https://static.igem.org/mediawiki/2015/d/d4/Freiburg_20150907_standard_curve.png" title="vergrößern"><img alt="" height="11" src="/igem2015/lib/plugins/imagebox/magnify-clip.png" width="15"/></a></div>Standard curve of sodium fluorescein diluted in 1x phosphate buffered saline. Data points are means of technical triplicates. Error bars indicated the standard deviation.</div></div></div> | ||
| + | <div> | ||
</p> | </p> | ||
<div class="tags"><span> | <div class="tags"><span> | ||
Latest revision as of 09:44, 7 September 2015
Raw-data of InterLab measurements
All data are provided in arbitrary units and were converted to absolute units using the fluorescein standard curve indicated below.
Top10, 10°C
Raw-data from the measurements of Top10 E. coli cells after incubation for 8h at 37°C and 8h at 10°C. Data are provided in relative fluorescence units as returned by the plate reader device.
| biol 1 | biol 2 | biol 3 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| tech 1 | tech 2 | tech 3 | tech 1 | tech 2 | tech 3 | tech 1 | tech 2 | tech 3 | |
| BBa_R0040 (neg) | 1716 | 1666 | 1674 | 1769 | 1730 | 1640 | 2348 | 2082 | 2141 |
| BBa_J23117 | 2402 | 2313 | 2331 | 2395 | 2196 | 2137 | 2503 | 2217 | 2362 |
| BBa_J23106 | 28320 | 26471 | 24464 | 27135 | 24487 | 26439 | 25224 | 23277 | 22407 |
| BBa_J23101 | 35948 | 33831 | 35038 | 44601 | 44093 | 42799 | 45802 | 42726 | 43200 |
| BBa_I20270 (pos) | 8662 | 8091 | 8376 | 9430 | 9292 | 8647 | 9335 | 9012 | 9001 |
Top10, 37°C
Raw-data from the measurements of Top10 E. coli cells after incubation for 16h at 37°C. Data are provided in relative fluorescence units as returned by the plate reader device.
| biol 1 | biol 2 | biol 3 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| tech 1 | tech 2 | tech 3 | tech 1 | tech 2 | tech 3 | tech 1 | tech 2 | tech 3 | |
| BBa_R0040 (neg) | 2528 | 2263 | 2313 | 2436 | 2404 | 2339 | 2448 | 2228 | 2303 |
| BBa_J23117 | 3243 | 3052 | 3045 | 2756 | 2596 | 2585 | 2710 | 2528 | 2392 |
| BBa_J23106 | 20476 | 20619 | 21054 | 21861 | 21553 | 21827 | 21310 | 19229 | 20067 |
| BBa_J23101 | 64236 | 62161 | 59427 | 59452 | 58348 | 57278 | 60200 | 56555 | 59515 |
| BBa_I20270 (pos) | 8518 | 8142 | 7977 | 7958 | 8317 | 7874 | 8506 | 8212 | 8800 |
ArcticExpress, 10°C
Raw-data from the measurements of Arctic Express E. coli cells after incubation for 8h at 37°C and 8h at 10°C. Data are provided in relative fluorescence units as returned by the plate reader device.
| biol 1 | biol 2 | biol 3 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| tech 1 | tech 2 | tech 3 | tech 1 | tech 2 | tech 3 | tech 1 | tech 2 | tech 3 | |
| BBa_R0040 (neg) | 2331 | 2632 | 2235 | 2089 | 1878 | 1878 | 2143 | 1988 | 2099 |
| BBa_J23117 | 2228 | 2211 | 2374 | 2577 | 2232 | 2475 | 2708 | 3043 | 2765 |
| BBa_J23106 | 20624 | 20162 | 21522 | 17971 | 19178 | 21544 | 18776 | 17977 | 18296 |
| BBa_J23101 | 40700 | 41753 | 43817 | 27887 | 27170 | 27771 | 32407 | 30150 | 32359 |
| BBa_I20270 (pos) | 14673 | 13975 | 13659 | 13511 | 12666 | 13579 | 13155 | 11919 | 13791 |
ArcticExpress, 37°C
Raw-data from the measurements of Top10 E. coli cells after incubation for 16h at 37°C. Data are provided in relative fluorescence units as returned by the plate reader device.
| biol 1 | biol 2 | biol 3 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| tech 1 | tech 2 | tech 3 | tech 1 | tech 2 | tech 3 | tech 1 | tech 2 | tech 3 | |
| BBa_R0040 (neg) | 2316 | 2086 | 2145 | 2285 | 2074 | 2145 | 2313 | 2362 | 2147 |
| BBa_J23117 | 2785 | 2728 | 2791 | 3058 | 3030 | 3107 | 3038 | 2744 | 2739 |
| BBa_J23106 | 27582 | 25867 | 26231 | 24454 | 23938 | 23621 | 20395 | 20235 | 20479 |
| BBa_J23101 | 42190 | 40473 | 41872 | 35679 | 32512 | 32793 | 34529 | 31809 | 35964 |
| BBa_I20270 (pos) | 14000 | 12792 | 12803 | 14657 | 14546 | 14124 | 13238 | 12286 | 12489 |
Fluorescein standard curve
The data points for 50 ng fluorescein were excluded from standard curve calculation.
| ng abs | 50 | 37,5 | 25 | 12,5 | 5 | 2,5 | 1 | 0,5 | 0 |
|---|---|---|---|---|---|---|---|---|---|
| 96905 | 76158 | 55023 | 26453 | 10852 | 5251 | 2498 | 1199 | 78 | |
| 99452 | 76967 | 54725 | 27348 | 11022 | 5764 | 2221 | 1290 | 54 | |
| OVRFLW | 76097 | 55356 | 28416 | 12539 | 6672 | 2737 | 2955 | 110 |
