Difference between revisions of "Team:EPF Lausanne/Part Collection"
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<h1>dCas9-ω</h1> | <h1>dCas9-ω</h1> | ||
| − | < | + | <h4>This part is the coding sequence of the dCas9-ω protein</h4> |
<p>dCa9s-ω is our main tool to build our project in <i>E. coli</i>. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.</p> | <p>dCa9s-ω is our main tool to build our project in <i>E. coli</i>. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.</p> | ||
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<h1>PAM rich URS J23117</h1> | <h1>PAM rich URS J23117</h1> | ||
| − | < | + | <h4>This part is promoter that can be used with our dCas9-ω</h4> |
<p>This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.</p> | <p>This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.</p> | ||
</div> | </div> | ||
Revision as of 10:09, 7 September 2015
dCas9-ω
This part is the coding sequence of the dCas9-ω protein
dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.
PAM rich URS J23117
This part is promoter that can be used with our dCas9-ω
This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.
