Difference between revisions of "Team:Freiburg/Project/pRIG15 7"
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To insert the sequence for Varicella Zoster glycoprotein E into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. This fragment was amplified via PCR (Link zum Labjournal-Eintrag) and then assembled with the digested pSB1C3 backbone using Gibson assembly. | To insert the sequence for Varicella Zoster glycoprotein E into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. This fragment was amplified via PCR (Link zum Labjournal-Eintrag) and then assembled with the digested pSB1C3 backbone using Gibson assembly. | ||
To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing. | To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing. | ||
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| + | Link to genebank file: <a class="media" href="https://static.igem.org/mediawiki/2015/3/34/Freiburg_2015_BBa_K1621001.gb" title="2015_Freiburg_BBa_K1621000" src="https://static.igem.org/mediawiki/2015/3/34/Freiburg_2015_BBa_K1621001.gb">BBa_K1621001.gb</a>. | ||
</p> | </p> | ||
</div> | </div> | ||
Revision as of 12:27, 7 September 2015
pRIG15_7
The Varicella Zoster Virus also called Human Herpes Virus Type 3 causes chickenpox after first infection and remains as a latent infection. The sequence we used during our project codes for the extracellular part of the glycoprotein E 1), which is the most abundant glycoprotein on the surface of infected cells. 2)
To insert the sequence for Varicella Zoster glycoprotein E into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. This fragment was amplified via PCR (Link zum Labjournal-Eintrag) and then assembled with the digested pSB1C3 backbone using Gibson assembly. To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing. Link to genebank file: BBa_K1621001.gb.
