Difference between revisions of "Team:EPF Lausanne/Part Collection"

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<h2>sgRNA Z0</h2>
 
<h2>sgRNA Z0</h2>
<p>the gRNA Z0 activates PAM rich URS J23117 promoter when in complex with dCas9.</p>
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<p>the gRNA Z0 inhibit PAM rich URS J23117 promoter when in complex with dCas9-ω.</p>
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<br><br><br><br><br>
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<a href="?"><b>BBa_TRUCMUCH</b></a>
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<img src="https://static.igem.org/mediawiki/2015/5/50/EPF_Lausanne_Parts_Z4.png" style="width:75%">
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<h2>sgRNA Z4</h2>
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<p>the gRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9.</p>
 
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Revision as of 16:23, 7 September 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

OUR PART COLLECTION

dCas9-ω

dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.

PAM rich URS J23117

This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.

sgRNA Z0

the gRNA Z0 inhibit PAM rich URS J23117 promoter when in complex with dCas9-ω.

sgRNA Z4

the gRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω.

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

NOT PROOFREAD